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Article

Prognostic Role of Circulating miRNAs in Early-Stage Non-Small Cell Lung Cancer

1
Biosciences Laboratory, Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS, 47014 Meldola, Italy
2
Biostatistics and Clinical Trials Unit, Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS, 47014 Meldola, Italy
3
Department of Medical Oncology, Santa Maria della Misericordia Hospital, Azienda Ospedaliera di Perugia, 06129 Perugia, Italy
4
General Pathology Unit, Department of Medicine, University of Perugia, 06132 Perugia, Italy
5
Department of Medical Oncology, Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS, 47014 Meldola, Italy
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
J. Clin. Med. 2019, 8(2), 131; https://doi.org/10.3390/jcm8020131
Received: 17 December 2018 / Revised: 17 January 2019 / Accepted: 20 January 2019 / Published: 23 January 2019
Non-small cell lung cancer (NSCLC) is the primary cause of cancer-related death worldwide, with a low 5-year survival rate even in fully resected early-stage disease. Novel biomarkers to identify patients at higher risk of relapse are needed. We studied the prognostic value of 84 circulating microRNAs (miRNAs) in 182 patients with resected early-stage NSCLC (99 adenocarcinoma (ADC), 83 squamous cell carcinoma (SCC)) from whom peripheral blood samples were collected pre-surgery. miRNA expression was analyzed in relation to disease-free survival (DFS) and overall survival (OS). In univariable analyses, five miRNAs (miR-26a-5p, miR-126-3p, miR-130b-3p, miR-205-5p, and miR-21-5p) were significantly associated with DFS in SCC, and four (miR-130b-3p, miR-26a-5p, miR-126-3p, and miR-205-5p) remained significantly associated with OS. In ADC, miR-222-3p, miR-22-3p, and mir-93-5p were significantly associated with DFS, miR-22-3p remaining significant for OS. Given the high-dimensionality of the dataset, multivariable models were obtained using a regularized Cox regression including all miRNAs and clinical covariates. After adjustment for disease stage, only miR-126-3p showed an independent prognostic role, with higher values associated with longer DFS in SCC patients. With regard to ADC and OS, no miRNA remained significant in multivariable analysis. Further investigation into the role of miR-126 as a prognostic marker in early-stage NSCLC is warranted. View Full-Text
Keywords: miRNAs; early-stage NSCLC; prognosis; plasma miRNAs; early-stage NSCLC; prognosis; plasma
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MDPI and ACS Style

Ulivi, P.; Petracci, E.; Marisi, G.; Baglivo, S.; Chiari, R.; Billi, M.; Canale, M.; Pasini, L.; Racanicchi, S.; Vagheggini, A.; Delmonte, A.; Mariotti, M.; Ludovini, V.; Bonafè, M.; Crinò, L.; Grignani, F. Prognostic Role of Circulating miRNAs in Early-Stage Non-Small Cell Lung Cancer. J. Clin. Med. 2019, 8, 131. https://doi.org/10.3390/jcm8020131

AMA Style

Ulivi P, Petracci E, Marisi G, Baglivo S, Chiari R, Billi M, Canale M, Pasini L, Racanicchi S, Vagheggini A, Delmonte A, Mariotti M, Ludovini V, Bonafè M, Crinò L, Grignani F. Prognostic Role of Circulating miRNAs in Early-Stage Non-Small Cell Lung Cancer. Journal of Clinical Medicine. 2019; 8(2):131. https://doi.org/10.3390/jcm8020131

Chicago/Turabian Style

Ulivi, Paola, Elisabetta Petracci, Giorgia Marisi, Sara Baglivo, Rita Chiari, Monia Billi, Matteo Canale, Luigi Pasini, Serena Racanicchi, Alessandro Vagheggini, Angelo Delmonte, Marita Mariotti, Vienna Ludovini, Massimiliano Bonafè, Lucio Crinò, and Francesco Grignani. 2019. "Prognostic Role of Circulating miRNAs in Early-Stage Non-Small Cell Lung Cancer" Journal of Clinical Medicine 8, no. 2: 131. https://doi.org/10.3390/jcm8020131

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