Genetic Determinants and Prediction of Antibiotic Resistance Phenotypes in Helicobacter pylori
AbstractHelicobacter pylori is a major human pathogen. Diagnosis of H. pylori infection and determination of its antibiotic susceptibility still mainly rely on culture and phenotypic drug susceptibility testing (DST) that is time-consuming and laborious. Whole genome sequencing (WGS) has recently emerged in medical microbiology as a diagnostic tool for reliable drug resistance prediction in bacterial pathogens. The aim of this study was to compare phenotypic DST results with the predictions based on the presence of genetic determinants identified in the H. pylori genome using WGS. Phenotypic resistance to clarithromycin, metronidazole, tetracycline, levofloxacin, and rifampicin was determined in 140 clinical H. pylori isolates by E-Test®, and the occurrence of certain single nucleotide polymorphisms (SNPs) in target genes was determined by WGS. Overall, there was a high congruence of >99% between phenotypic DST results for clarithromycin, levofloxacin, and rifampicin and SNPs identified in the 23S rRNA, gyrA, and rpoB gene. However, it was not possible to infer a resistance phenotype for metronidazole based on the occurrence of distinct SNPs in frxA and rdxA. All 140 H. pylori isolates analysed in this study were susceptible to tetracycline, which was in accordance with the absence of double or triple nucleotide substitutions in the 16S rRNA gene. View Full-Text
- Supplementary File 1:
DOCX-Document (DOCX, 4033 KB)
Share & Cite This Article
Lauener, F.N.; Imkamp, F.; Lehours, P.; Buissonnière, A.; Benejat, L.; Zbinden, R.; Keller, P.M.; Wagner, K. Genetic Determinants and Prediction of Antibiotic Resistance Phenotypes in Helicobacter pylori. J. Clin. Med. 2019, 8, 53.
Lauener FN, Imkamp F, Lehours P, Buissonnière A, Benejat L, Zbinden R, Keller PM, Wagner K. Genetic Determinants and Prediction of Antibiotic Resistance Phenotypes in Helicobacter pylori. Journal of Clinical Medicine. 2019; 8(1):53.Chicago/Turabian Style
Lauener, Francis N.; Imkamp, Frank; Lehours, Philippe; Buissonnière, Alice; Benejat, Lucie; Zbinden, Reinhard; Keller, Peter M.; Wagner, Karoline. 2019. "Genetic Determinants and Prediction of Antibiotic Resistance Phenotypes in Helicobacter pylori." J. Clin. Med. 8, no. 1: 53.
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.