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Article

Cellular Processes in Human Ovarian Follicles Are Regulated by Expression Profile of New Gene Markers—Clinical Approach

1
Department of Gynecology, Division of Infertility and Reproductive Endocrinology, Obstetrics and Gynecological Oncology, Poznan University of Medical Sciences, 33 Polna St., 60-535 Poznan, Poland
2
Department of Anatomy, Poznan University of Medical Sciences, 6 Swiecickiego St., 60-781 Poznan, Poland
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Department of Histology and Embryology, Poznan University of Medical Sciences, 6 Swiecickiego St., 60-781 Poznan, Poland
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Department of Toxicology, Poznan University of Medical Sciences, 30 Dojazd St., 60-631 Poznan, Poland
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Department of Basic and Preclinical Sciences, Institute of Veterinary Medicine, Nicolaus Copernicus University in Toruń, 7 Gagarina St., 87-100 Torun, Poland
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Physiology Graduate Program, North Carolina State University, Raleigh, NC 27695, USA
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Prestage Department of Poultry Science, North Carolina State University, Raleigh, NC 27695, USA
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Department of Veterinary Surgery, Institute of Veterinary Medicine, Nicolaus Copernicus University in Torun, 1 Lwowska St., 87-100 Torun, Poland
*
Author to whom correspondence should be addressed.
Academic Editor: Jacek Szamatowicz
J. Clin. Med. 2022, 11(1), 73; https://doi.org/10.3390/jcm11010073
Received: 15 November 2021 / Revised: 18 December 2021 / Accepted: 20 December 2021 / Published: 24 December 2021
(This article belongs to the Special Issue Updates in Diagnosis and Treatment of Infertility)
In the growing ovarian follicle, the maturing oocyte is accompanied by cumulus (CCs) and granulosa (GCs) cells. Currently, there remain many unanswered questions about the epithelial origin of these cells. Global and targeted gene transcript levels were assessed on 1, 7, 15, 30 days of culture for CCs and GCs. Detailed analysis of the genes belonging to epithelial cell-associated ontological groups allowed us to assess a total of 168 genes expressed in CCs (97 genes) and GCs (71 genes) during long-term in vitro culture. Expression changes of the analyzed genes allowed the identification of the group of genes: TGFBR3, PTGS2, PRKX, AHI1, and IL11, whose expression decreased the most and the group of ANXA3, DKK1, CCND1, STC1, CAV1, and SFRP4 genes, whose expression significantly increased. These genes’ expression indicates CCs and GCs epithelialization processes and their epithelial origin. Expression change analysis of genes involved in epithelization processes in GCs and CCs during their in vitro culture made it possible to describe the most significantly altered of the 11 genes. Detailed analysis of gene expression in these two cell populations at different time intervals confirms their ovarian surface epithelial origin. Furthermore, some gene expression profiles appear to have tumorigenic properties, suggesting that granulosa cells may play a role in cancerogenesis. View Full-Text
Keywords: cumulus cells; granulosa cells; human; gene expression; microarray; epithelialization cumulus cells; granulosa cells; human; gene expression; microarray; epithelialization
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MDPI and ACS Style

Chermuła, B.; Kranc, W.; Celichowski, P.; Stelmach, B.; Piotrowska-Kempisty, H.; Mozdziak, P.; Pawelczyk, L.; Spaczyński, R.Z.; Kempisty, B. Cellular Processes in Human Ovarian Follicles Are Regulated by Expression Profile of New Gene Markers—Clinical Approach. J. Clin. Med. 2022, 11, 73. https://doi.org/10.3390/jcm11010073

AMA Style

Chermuła B, Kranc W, Celichowski P, Stelmach B, Piotrowska-Kempisty H, Mozdziak P, Pawelczyk L, Spaczyński RZ, Kempisty B. Cellular Processes in Human Ovarian Follicles Are Regulated by Expression Profile of New Gene Markers—Clinical Approach. Journal of Clinical Medicine. 2022; 11(1):73. https://doi.org/10.3390/jcm11010073

Chicago/Turabian Style

Chermuła, Błażej, Wiesława Kranc, Piotr Celichowski, Bogusława Stelmach, Hanna Piotrowska-Kempisty, Paul Mozdziak, Leszek Pawelczyk, Robert Z. Spaczyński, and Bartosz Kempisty. 2022. "Cellular Processes in Human Ovarian Follicles Are Regulated by Expression Profile of New Gene Markers—Clinical Approach" Journal of Clinical Medicine 11, no. 1: 73. https://doi.org/10.3390/jcm11010073

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