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Article

Extracellular Vesicles Tropism: A Comparative Study between Passive Innate Tropism and the Active Engineered Targeting Capability of Lymphocyte-Derived EVs

1
Department of Applied Science and Technology, Politecnico di Torino, Corso Duca degli Abruzzi 24, 10129 Turin, Italy
2
Department of Neuroscience, Rehabilitation, Ophthalmology, Genetics, Maternal and Child Health, University of Genova, 16132 Genova, Italy
3
Department of Pediatrics, IRCCS Istituto Giannina Gaslini, University of Genova, 16128 Genova, Italy
4
Electron Microscopy Laboratory, Center for Convergent Technologies, Istituto Italiano di Tecnologia (IIT), Via Morego 30, 16163 Genoa, Italy
*
Authors to whom correspondence should be addressed.
Equal contribution.
Academic Editor: Shiro Suetsugu
Membranes 2021, 11(11), 886; https://doi.org/10.3390/membranes11110886
Received: 30 October 2021 / Revised: 12 November 2021 / Accepted: 16 November 2021 / Published: 18 November 2021
Cellular communications take place thanks to a well-connected network of chemical–physical signals, biomolecules, growth factors, and vesicular messengers that travel inside or between cells. A deep knowledge of the extracellular vesicle (EV) system allows for a better understanding of the whole series of phenomena responsible for cell proliferation and death. To this purpose, here, a thorough immuno-phenotypic characterization of B-cell EV membranes is presented. Furthermore, the cellular membrane of B lymphocytes, Burkitt lymphoma, and human myeloid leukemic cells were characterized through cytofluorimetry assays and fluorescent microscopy analysis. Through cytotoxicity and internalization tests, the tropism of B lymphocyte-derived EVs was investigated toward the parental cell line and two different cancer cell lines. In this study, an innate capability of passive targeting of the native EVs was distinguished from the active targeting capability of monoclonal antibody-engineered EVs, able to selectively drive the vesicles, enhancing their internalization into the target cancer cells. In particular, the specific targeting ability of anti-CD20 engineered EVs towards Daudi cells, highly expressing CD20 marker on their cell membrane, was proved, while almost no internalization events were observed in HL60 cells, since they did not express an appreciable amount of the CD20 marker on their plasma membranes. View Full-Text
Keywords: extracellular vesicles; B lymphocytes; human myeloid leukemia; Burkitt lymphoma; surface labeling; cellular uptake; cell tropism; cell targeting extracellular vesicles; B lymphocytes; human myeloid leukemia; Burkitt lymphoma; surface labeling; cellular uptake; cell tropism; cell targeting
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MDPI and ACS Style

Limongi, T.; Susa, F.; Dumontel, B.; Racca, L.; Perrone Donnorso, M.; Debellis, D.; Cauda, V. Extracellular Vesicles Tropism: A Comparative Study between Passive Innate Tropism and the Active Engineered Targeting Capability of Lymphocyte-Derived EVs. Membranes 2021, 11, 886. https://doi.org/10.3390/membranes11110886

AMA Style

Limongi T, Susa F, Dumontel B, Racca L, Perrone Donnorso M, Debellis D, Cauda V. Extracellular Vesicles Tropism: A Comparative Study between Passive Innate Tropism and the Active Engineered Targeting Capability of Lymphocyte-Derived EVs. Membranes. 2021; 11(11):886. https://doi.org/10.3390/membranes11110886

Chicago/Turabian Style

Limongi, Tania, Francesca Susa, Bianca Dumontel, Luisa Racca, Michela Perrone Donnorso, Doriana Debellis, and Valentina Cauda. 2021. "Extracellular Vesicles Tropism: A Comparative Study between Passive Innate Tropism and the Active Engineered Targeting Capability of Lymphocyte-Derived EVs" Membranes 11, no. 11: 886. https://doi.org/10.3390/membranes11110886

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