DMPC Phospholipid Bilayer as a Potential Interface for Human Cystatin C Oligomerization: Analysis of Protein-Liposome Interactions Using NMR Spectroscopy

Jurczak, Przemyslaw; Szutkowski, Kosma; Lach, Slawomir; Jurga, Stefan; Czaplewska, Paulina; Szymanska, Aneta; Zhukov, Igor

doi:10.3390/membranes11010013

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DMPC Phospholipid Bilayer as a Potential Interface for Human Cystatin C Oligomerization: Analysis of Protein-Liposome Interactions Using NMR Spectroscopy

and

^4,*

Faculty of Chemistry, University of Gdańsk, Wita Stwosza 63, 80-308 Gdańsk, Poland

NanoBioMedical Centre, Adam Mickiewicz University, Wszechnicy Piastowskiej 3, 61-614 Poznań, Poland

Intercollegiate Faculty of Biotechnology UG & MUG, University of Gdańsk, Gdańsk, Abrahama 58, 80-307 Gdańsk, Poland

⁴

Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Adolfa Pawińskiego 5A, 02-106 Warszawa, Poland

Author to whom correspondence should be addressed.

Membranes 2021, 11(1), 13; https://doi.org/10.3390/membranes11010013

Received: 29 November 2020 / Revised: 14 December 2020 / Accepted: 18 December 2020 / Published: 24 December 2020

(This article belongs to the Special Issue Biomolecules in Cell Membranes: Structure and Dynamics)

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Abstract

Studies revolving around mechanisms responsible for the development of amyloid-based diseases lay the foundations for the recognition of molecular targets of future to-be-developed treatments. However, the vast number of peptides and proteins known to be responsible for fibril formation, combined with their complexity and complexity of their interactions with various cellular components, renders this task extremely difficult and time-consuming. One of these proteins, human cystatin C (hCC), is a well-known and studied cysteine-protease inhibitor. While being a monomer in physiological conditions, under the necessary stimulus—usually a mutation, it tends to form fibrils, which later participate in the disease development. This process can potentially be regulated (in several ways) by many cellular components and it is being hypothesized that the cell membrane might play a key role in the oligomerization pathway. Studies involving cell membranes pose several difficulties; therefore, an alternative in the form of membrane mimetics is a very attractive solution. Here, we would like to present the first study on hCC oligomerization under the influence of phospholipid liposomes, acting as a membrane mimetic. The protein–mimetic interactions are studied utilizing circular dichroism, nuclear magnetic resonance, and size exclusion chromatography. View Full-Text

Keywords: human cystatin C; NMR spectroscopy; ¹⁵N relaxation; phospholipid; DMPC; liposome; protein–liposome interaction

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Jurczak, P.; Szutkowski, K.; Lach, S.; Jurga, S.; Czaplewska, P.; Szymanska, A.; Zhukov, I. DMPC Phospholipid Bilayer as a Potential Interface for Human Cystatin C Oligomerization: Analysis of Protein-Liposome Interactions Using NMR Spectroscopy. Membranes 2021, 11, 13. https://doi.org/10.3390/membranes11010013

AMA Style

Jurczak P, Szutkowski K, Lach S, Jurga S, Czaplewska P, Szymanska A, Zhukov I. DMPC Phospholipid Bilayer as a Potential Interface for Human Cystatin C Oligomerization: Analysis of Protein-Liposome Interactions Using NMR Spectroscopy. Membranes. 2021; 11(1):13. https://doi.org/10.3390/membranes11010013

Chicago/Turabian Style

Jurczak, Przemyslaw, Kosma Szutkowski, Slawomir Lach, Stefan Jurga, Paulina Czaplewska, Aneta Szymanska, and Igor Zhukov. 2021. "DMPC Phospholipid Bilayer as a Potential Interface for Human Cystatin C Oligomerization: Analysis of Protein-Liposome Interactions Using NMR Spectroscopy" Membranes 11, no. 1: 13. https://doi.org/10.3390/membranes11010013

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DMPC Phospholipid Bilayer as a Potential Interface for Human Cystatin C Oligomerization: Analysis of Protein-Liposome Interactions Using NMR Spectroscopy

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