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Open AccessArticle

BEI Inactivated Vaccine Induces Innate and Adaptive Responses and Elicits Partial Protection upon Reassortant Betanodavirus Infection in Senegalese Sole

Departamento de Microbiología y Parasitología, Campus Vida, Instituto de Acuicultura, Universidade de Santiago de Compostela, 15782 Santiago de Compostela, Spain
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Academic Editors: Beatriz Novoa and Patricia Pereiro
Vaccines 2021, 9(5), 458; https://doi.org/10.3390/vaccines9050458 (registering DOI)
Received: 29 March 2021 / Revised: 28 April 2021 / Accepted: 29 April 2021 / Published: 4 May 2021
(This article belongs to the Special Issue Vaccines for Aquaculture)
Nervous necrosis virus (NNV), the causative agent of viral encephalopathy and retinopathy (VER), is one of the most threatening viruses affecting marine and freshwater fish species worldwide. Senegalese sole is a promising fish species in Mediterranean aquaculture but also highly susceptible to NNV and VER outbreaks, that puts its farming at risk. The development of vaccines for aquaculture is one of best tools to prevent viral spread and sudden outbreaks, and virus inactivation is the simplest and most cost-effective method available. In this work, we have designed two inactivated vaccines based on the use of formalin or binary ethylenimine (BEI) to inactivate a reassortant NNV strain. After vaccination, the BEI-inactivated vaccine triggered the production of specific IgM-NNV antibodies and stimulated innate and adaptive immune responses at transcriptional level (rtp3, mx, mhcii and tcrb coding genes). Moreover, it partially improved survival after an NNV in vivo challenge, reducing the mid-term viral load and avoiding the down-regulation of immune response post-challenge. On the other hand, the formalin-inactivated vaccine improved the survival of fish upon infection without inducing the production of IgM-NNV antibodies and only stimulating the expression of herc4 and mhcii genes (in head-kidney and brain, respectively) during the vaccination period; this suggests that other immune-related pathways may be involved in the partial protection provoked. Although these vaccines against NNV showed encouraging results, further studies are needed to improve sole protection and to fully understand the underlying immune mechanism. View Full-Text
Keywords: Senegalese sole; inactivated vaccine; BEI; formalin; nervous necrosis virus; immune response; antibodies; gene expression Senegalese sole; inactivated vaccine; BEI; formalin; nervous necrosis virus; immune response; antibodies; gene expression
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MDPI and ACS Style

Valero, Y.; Olveira, J.G.; López-Vázquez, C.; Dopazo, C.P.; Bandín, I. BEI Inactivated Vaccine Induces Innate and Adaptive Responses and Elicits Partial Protection upon Reassortant Betanodavirus Infection in Senegalese Sole. Vaccines 2021, 9, 458. https://doi.org/10.3390/vaccines9050458

AMA Style

Valero Y, Olveira JG, López-Vázquez C, Dopazo CP, Bandín I. BEI Inactivated Vaccine Induces Innate and Adaptive Responses and Elicits Partial Protection upon Reassortant Betanodavirus Infection in Senegalese Sole. Vaccines. 2021; 9(5):458. https://doi.org/10.3390/vaccines9050458

Chicago/Turabian Style

Valero, Yulema; Olveira, José G.; López-Vázquez, Carmen; Dopazo, Carlos P.; Bandín, Isabel. 2021. "BEI Inactivated Vaccine Induces Innate and Adaptive Responses and Elicits Partial Protection upon Reassortant Betanodavirus Infection in Senegalese Sole" Vaccines 9, no. 5: 458. https://doi.org/10.3390/vaccines9050458

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