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Article

Fusion Cytokines IL-7-Linker-IL-15 Promote Mycobacterium Tuberculosis Subunit Vaccine to Induce Central Memory like T Cell-Mediated Immunity

1
Gansu Provincial Key Laboratory of Evidence Based Medicine and Clinical Translation & Lanzhou Center for Tuberculosis Research, School of Basic Medical Sciences, Lanzhou University, Lanzhou 730000, China
2
Institute of Pathogen Biology, School of Basic Medical Sciences, Lanzhou University, Lanzhou 730000, China
*
Author to whom correspondence should be addressed.
Vaccines 2020, 8(4), 715; https://doi.org/10.3390/vaccines8040715
Received: 31 October 2020 / Revised: 25 November 2020 / Accepted: 28 November 2020 / Published: 1 December 2020
(This article belongs to the Special Issue Vaccines and Other Antibody Therapies Against Bacterial Infection)
Tuberculosis (TB), caused by Mycobacterium tuberculosis (M. tuberculosis), is among the most serious infectious diseases worldwide. Adjuvanted protein subunit vaccines have been demonstrated as a kind of promising novel vaccine. This study proposed to investigate whether cytokines interliukine-7 (IL-7) and interliukine-15 (IL-15) help TB subunit vaccines induce long-term cell-mediated immune responses, which are required for vaccination against TB. In this study, mice were immunized with the M. tuberculosis protein subunit vaccines combined with adnovirus-mediated cytokines IL-7, IL-15, IL-7-IL-15, and IL-7-Linker-IL-15 at 0, 2, and 4 weeks, respectively. Twenty weeks after the last immunization, the long-term immune responses, especially the central memory-like T cells (TCM like cell)-mediated immune responses, were determined with the methods of cultured IFN-γ-ELISPOT, expanded secondary immune responses, cell proliferation, and protective efficacy against Mycobacterium bovis Bacilli Calmette-Guerin (BCG) challenge, etc. The results showed that the group of vaccine + rAd-IL-7-Linker-IL-15 induced a stronger long-term antigen-specific TCM like cells-mediated immune responses and had higher protective efficacy against BCG challenge than the vaccine + rAd-vector control group, the vaccine + rAd-IL-7 and the vaccine + rAd-IL-15 groups. This study indicated that rAd-IL-7-Linker-IL-15 improved the TB subunit vaccine’s efficacy by augmenting TCM like cells and provided long-term protective efficacy against Mycobacteria. View Full-Text
Keywords: M. tuberculosis; subunit vaccine; fusion cytokines; IL-7-Linker-IL-15; central memory like T cells M. tuberculosis; subunit vaccine; fusion cytokines; IL-7-Linker-IL-15; central memory like T cells
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MDPI and ACS Style

Bai, C.; Zhou, L.; Tang, J.; He, J.; Han, J.; Niu, H.; Zhu, B. Fusion Cytokines IL-7-Linker-IL-15 Promote Mycobacterium Tuberculosis Subunit Vaccine to Induce Central Memory like T Cell-Mediated Immunity. Vaccines 2020, 8, 715. https://doi.org/10.3390/vaccines8040715

AMA Style

Bai C, Zhou L, Tang J, He J, Han J, Niu H, Zhu B. Fusion Cytokines IL-7-Linker-IL-15 Promote Mycobacterium Tuberculosis Subunit Vaccine to Induce Central Memory like T Cell-Mediated Immunity. Vaccines. 2020; 8(4):715. https://doi.org/10.3390/vaccines8040715

Chicago/Turabian Style

Bai, Chunxiang, Lijun Zhou, Junxia Tang, Juanjuan He, Jiangyuan Han, Hongxia Niu, and Bingdong Zhu. 2020. "Fusion Cytokines IL-7-Linker-IL-15 Promote Mycobacterium Tuberculosis Subunit Vaccine to Induce Central Memory like T Cell-Mediated Immunity" Vaccines 8, no. 4: 715. https://doi.org/10.3390/vaccines8040715

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