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Vaccines
Volume 8
Issue 4
10.3390/vaccines8040716
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Article

Recombinant Live Attenuated Influenza Virus Expressing Conserved G-Protein Domain in a Chimeric Hemagglutinin Molecule Induces G-Specific Antibodies and Confers Protection against Respiratory Syncytial Virus

by
Yu-Jin Jung
1,
Yu-Na Lee
1,2,
Ki-Hye Kim
1,
Youri Lee
1,
Subbiah Jeeva
1,
Bo Ryoung Park
1 and
Sang-Moo Kang
1,*
1
Center for Inflammation, Immunity & Infection, Institute for Biomedical Sciences, Georgia State University, Atlanta, GA 30303, USA
2
Animal and Plant Quarantine Agency, Gimcheon, Gyeongsangbukdo 39660, Korea
*
Author to whom correspondence should be addressed.
Vaccines 2020, 8(4), 716; https://doi.org/10.3390/vaccines8040716
Submission received: 10 October 2020 / Revised: 4 November 2020 / Accepted: 13 November 2020 / Published: 1 December 2020
(This article belongs to the Special Issue Evaluation of Vaccine Immunogenicity)
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Abstract

Respiratory syncytial virus (RSV) is one of the most important pathogens causing significant morbidity and mortality in infants and the elderly. Live attenuated influenza vaccine (LAIV) is a licensed vaccine platform in humans and it is known to induce broader immune responses. RSV G attachment proteins mediate virus binding to the target cells and they contain a conserved central domain with neutralizing epitopes. Here, we generated recombinant LAIV based on the attenuated A/Puerto Rico/8/1934 virus backbone, expressing an RSV conserved G-domain in a chimeric hemagglutinin (HA) fusion molecule (HA-G). The attenuated phenotypes of chimeric HA-G LAIV were evident by restricted replication in the upper respiratory tract and low temperature growth characteristics. The immunization of mice with chimeric HA-G LAIV induced significant increases in G-protein specific IgG2a (T helper type 1) and IgG antibody-secreting cell responses in lung, bronchioalveolar fluid, bone marrow, and spleens after RSV challenge. Vaccine-enhanced disease that is typically caused by inactivated-RSV vaccination was not observed in chimeric HA-G LAIV as analyzed by lung histopathology. These results in this study suggest a new approach of developing an RSV vaccine candidate while using recombinant LAIV, potentially conferring protection against influenza virus and RSV.
Keywords: recombinant LAIV. RSV G; chimeric hemagglutinin recombinant LAIV. RSV G; chimeric hemagglutinin

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MDPI and ACS Style

Jung, Y.-J.; Lee, Y.-N.; Kim, K.-H.; Lee, Y.; Jeeva, S.; Park, B.R.; Kang, S.-M. Recombinant Live Attenuated Influenza Virus Expressing Conserved G-Protein Domain in a Chimeric Hemagglutinin Molecule Induces G-Specific Antibodies and Confers Protection against Respiratory Syncytial Virus. Vaccines 2020, 8, 716. https://doi.org/10.3390/vaccines8040716

AMA Style

Jung Y-J, Lee Y-N, Kim K-H, Lee Y, Jeeva S, Park BR, Kang S-M. Recombinant Live Attenuated Influenza Virus Expressing Conserved G-Protein Domain in a Chimeric Hemagglutinin Molecule Induces G-Specific Antibodies and Confers Protection against Respiratory Syncytial Virus. Vaccines. 2020; 8(4):716. https://doi.org/10.3390/vaccines8040716

Chicago/Turabian Style

Jung, Yu-Jin, Yu-Na Lee, Ki-Hye Kim, Youri Lee, Subbiah Jeeva, Bo Ryoung Park, and Sang-Moo Kang. 2020. "Recombinant Live Attenuated Influenza Virus Expressing Conserved G-Protein Domain in a Chimeric Hemagglutinin Molecule Induces G-Specific Antibodies and Confers Protection against Respiratory Syncytial Virus" Vaccines 8, no. 4: 716. https://doi.org/10.3390/vaccines8040716

APA Style

Jung, Y.-J., Lee, Y.-N., Kim, K.-H., Lee, Y., Jeeva, S., Park, B. R., & Kang, S.-M. (2020). Recombinant Live Attenuated Influenza Virus Expressing Conserved G-Protein Domain in a Chimeric Hemagglutinin Molecule Induces G-Specific Antibodies and Confers Protection against Respiratory Syncytial Virus. Vaccines, 8(4), 716. https://doi.org/10.3390/vaccines8040716

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