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Article

A GMMA-CPS-Based Vaccine for Non-Typhoidal Salmonella

1
Vaccine and Infectious Disease Organization-International Vaccine Centre, Saskatoon, SK S7N5E3, Canada
2
Department of Biochemistry, Microbiology and Immunology, University of Saskatchewan, Saskatoon, SK S7N5E5, Canada
3
Agriculture and Agri-Food Canada, Lethbridge Research and Development Centre, Lethbridge, AB T1J4B1, Canada
4
Department of Veterinary Biochemistry, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati 781022, Assam, India
*
Author to whom correspondence should be addressed.
Academic Editors: Ralph A. Tripp and Jason L. Larabee
Vaccines 2021, 9(2), 165; https://doi.org/10.3390/vaccines9020165
Received: 13 November 2020 / Revised: 10 February 2021 / Accepted: 11 February 2021 / Published: 17 February 2021
(This article belongs to the Special Issue Vaccines and Other Antibody Therapies Against Bacterial Infection)
Non-typhoidal Salmonella are a major cause of gastroenteritis worldwide, as well as causing bloodstream infections in sub-Saharan Africa with a high fatality rate. No vaccine is currently available for human use. Current vaccine development strategies are focused on capsular polysaccharides (CPS) present on the surface of non-typhoidal Salmonella. This study aimed to boost the amount of CPS purified from S. Typhimurium for immunization trials. Random mutagenesis with Tn10 transposon increased the production of CPS colanic acid, by 10-fold compared to wildtype. Immunization with colanic acid or colanic acid conjugated to truncated glycoprotein D or inactivated diphtheria toxin did not induce a protective immune response in mice. However, immunization with Generalized Modules for Membrane Antigens (GMMAs) isolated from colanic acid overproducing isolates reduced Salmonella colonization in mice. Our results support the development of a GMMA-CPS-based vaccine against non-typhoidal Salmonella. View Full-Text
Keywords: vaccines; Salmonella; capsular polysaccharide; colanic acid; GMMAs vaccines; Salmonella; capsular polysaccharide; colanic acid; GMMAs
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MDPI and ACS Style

Sokaribo, A.S.; Perera, S.R.; Sereggela, Z.; Krochak, R.; Balezantis, L.R.; Xing, X.; Lam, S.; Deck, W.; Attah-Poku, S.; Abbott, D.W.; Tamuly, S.; White, A.P. A GMMA-CPS-Based Vaccine for Non-Typhoidal Salmonella. Vaccines 2021, 9, 165. https://doi.org/10.3390/vaccines9020165

AMA Style

Sokaribo AS, Perera SR, Sereggela Z, Krochak R, Balezantis LR, Xing X, Lam S, Deck W, Attah-Poku S, Abbott DW, Tamuly S, White AP. A GMMA-CPS-Based Vaccine for Non-Typhoidal Salmonella. Vaccines. 2021; 9(2):165. https://doi.org/10.3390/vaccines9020165

Chicago/Turabian Style

Sokaribo, Akosiererem S., Sumudu R. Perera, Zoe Sereggela, Ryan Krochak, Lindsay R. Balezantis, Xiaohui Xing, Shirley Lam, William Deck, Sam Attah-Poku, Dennis W. Abbott, Shantanu Tamuly, and Aaron P. White. 2021. "A GMMA-CPS-Based Vaccine for Non-Typhoidal Salmonella" Vaccines 9, no. 2: 165. https://doi.org/10.3390/vaccines9020165

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