Enhancing Dengue Virus Production and Immunogenicity with Celcradle™ Bioreactor: A Comparative Study with Traditional Cell Culture Methods
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Line, Virus Strain, and Bioreactor
2.2. Cell Culture in Petri Dishes
2.3. Infection and Proliferation of DENV4 in Petri Dishes
2.4. Adaptation of Vero Cells to the Celcradle™ Bioreactor System
2.5. DENV4 Infection and Proliferation in the Celcradle™ Bioreactor System
2.6. Monitoring of Glucose
2.7. Monitoring of pH
2.8. Monitoring of Cell Count
2.9. Monitoring of Virus RNA
2.10. Purification and Concentration of Virus and Detection of Protein
2.11. Animal Immunization
2.12. Detection of Antibodies Titer
2.13. Statistical Analysis
3. Results
3.1. Adaptive Growth of Vero Cells on Suspended Carriers in the CelCradleTM Bioreactor System
3.2. DENV Proliferation through Vero Cells in the CelCradleTM Bioreactor System
3.3. Immunogenicity of DENV Produced by the CelCradleTM Bioreactor System
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Guo, H.; Ding, X.; Hua, D.; Liu, M.; Yang, M.; Gong, Y.; Ye, N.; Chen, X.; He, J.; Zhang, Y.; et al. Enhancing Dengue Virus Production and Immunogenicity with Celcradle™ Bioreactor: A Comparative Study with Traditional Cell Culture Methods. Vaccines 2024, 12, 563. https://doi.org/10.3390/vaccines12060563
Guo H, Ding X, Hua D, Liu M, Yang M, Gong Y, Ye N, Chen X, He J, Zhang Y, et al. Enhancing Dengue Virus Production and Immunogenicity with Celcradle™ Bioreactor: A Comparative Study with Traditional Cell Culture Methods. Vaccines. 2024; 12(6):563. https://doi.org/10.3390/vaccines12060563
Chicago/Turabian StyleGuo, Hongxia, Xiaoyan Ding, Dong Hua, Minchi Liu, Maocheng Yang, Yuanxin Gong, Nan Ye, Xiaozhong Chen, Jiuxiang He, Yu Zhang, and et al. 2024. "Enhancing Dengue Virus Production and Immunogenicity with Celcradle™ Bioreactor: A Comparative Study with Traditional Cell Culture Methods" Vaccines 12, no. 6: 563. https://doi.org/10.3390/vaccines12060563