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Antioxidants
Volume 9
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10.3390/antiox9010016
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Article

Piceatannol Increases Antioxidant Defense and Reduces Cell Death in Human Periodontal Ligament Fibroblast under Oxidative Stress

by
Flávia Póvoa da Costa
1,2,
Bruna Puty
1,2,
Lygia S. Nogueira
1,2,
Geovanni Pereira Mitre
3,
Sávio Monteiro dos Santos
4,
Bruno José Brito Teixeira
5,
Maria Sueli da Silva Kataoka
3,
Manoela Domingues Martins
6,
Carlos Augusto Galvão Barboza
7,
Marta Chagas Monteiro
4,
Hervé Rogez
5,
Edivaldo Herculano Corrêa de Oliveira
2 and
Rafael Rodrigues Lima
1,*
1
Laboratory of Functional and Structural Biology, Institute of Biological Sciences, Federal University of Pará (UFPA), Belém-Pará 66075-110, Brazil
2
Laboratory of Tissue Culture and Cytogenetics, Environment Section, Evandro Chagas Institute, Ananindeua-Pará 67030-000, Brazil
3
Laboratory of Cell Culture, Faculty of Dentistry, Federal University of Pará (UFPA), Belém-Pará 66075-110, Brazil
4
Laboratory of Oxidative Stress and Clinical Immunology, Faculty of Pharmacy, Federal University of Pará (UFPA), Belém-Pará 66075-110, Brazil
5
Center for Valorization of Amazonian Bioactive Compounds (CVACBA) & Federal University of Pará UFPA, Belém-Pará 66075-110, Brazil
6
Department of Oral Pathology, School of Dentistry, Federal University of Rio Grande do Sul, Porto Alegre 91509-900, RS, Brazil
7
Department of Morphology, Federal University of Rio Grande do Norte, Natal 59078-970, Brazil
*
Author to whom correspondence should be addressed.
Antioxidants 2020, 9(1), 16; https://doi.org/10.3390/antiox9010016
Submission received: 22 November 2019 / Accepted: 3 December 2019 / Published: 23 December 2019
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Abstract

Piceatannol is a resveratrol metabolite that is considered a potent antioxidant and cytoprotector because of its high capacity to chelate/sequester reactive oxygen species. In pathogenesis of periodontal diseases, the imbalance of reactive oxygen species is closely related to the disorder in the cells and may cause changes in cellular metabolism and mitochondrial activity, which is implicated in oxidative stress status or even in cell death. In this way, this study aimed to evaluate piceatannol as cytoprotector in culture of human periodontal ligament fibroblasts through in vitro analyses of cell viability and oxidative stress parameters after oxidative stress induced as an injury simulator. Fibroblasts were seeded and divided into the following study groups: control, vehicle, control piceatannol, H2O2 exposure, and H2O2 exposure combined with the maintenance in piceatannol ranging from 0.1 to 20 μM. The parameters analyzed following exposure were cell viability by trypan blue exclusion test, general metabolism status by the 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) method, mitochondrial activity through the ATP production, total antioxidant capacity, and reduced gluthatione. Piceatannol was shown to be cytoprotective due the maintenance of cell viability between 1 and 10 μM even in the presence of H2O2. In a concentration of 0.1 μM piceatannol decreased significantly cell viability but increased cellular metabolism and antioxidant capacity of the fibroblasts. On the other hand, the fibroblasts treated with piceatannol at 1 μM presented low metabolism and antioxidant capacity. However, piceatannol did not protect cells from mitochondrial damage as measured by ATP production. In summary, piceatannol is a potent antioxidant in low concentrations with cytoprotective capacity, but it does not prevent all damage caused by hydrogen peroxide.
Keywords: stilbenes; viability; hydrogen peroxide; piceatannol; periodontal ligament stilbenes; viability; hydrogen peroxide; piceatannol; periodontal ligament
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MDPI and ACS Style

Costa, F.P.d.; Puty, B.; Nogueira, L.S.; Mitre, G.P.; Santos, S.M.d.; Teixeira, B.J.B.; Kataoka, M.S.d.S.; Martins, M.D.; Barboza, C.A.G.; Monteiro, M.C.; et al. Piceatannol Increases Antioxidant Defense and Reduces Cell Death in Human Periodontal Ligament Fibroblast under Oxidative Stress. Antioxidants 2020, 9, 16. https://doi.org/10.3390/antiox9010016

AMA Style

Costa FPd, Puty B, Nogueira LS, Mitre GP, Santos SMd, Teixeira BJB, Kataoka MSdS, Martins MD, Barboza CAG, Monteiro MC, et al. Piceatannol Increases Antioxidant Defense and Reduces Cell Death in Human Periodontal Ligament Fibroblast under Oxidative Stress. Antioxidants. 2020; 9(1):16. https://doi.org/10.3390/antiox9010016

Chicago/Turabian Style

Costa, Flávia Póvoa da, Bruna Puty, Lygia S. Nogueira, Geovanni Pereira Mitre, Sávio Monteiro dos Santos, Bruno José Brito Teixeira, Maria Sueli da Silva Kataoka, Manoela Domingues Martins, Carlos Augusto Galvão Barboza, Marta Chagas Monteiro, and et al. 2020. "Piceatannol Increases Antioxidant Defense and Reduces Cell Death in Human Periodontal Ligament Fibroblast under Oxidative Stress" Antioxidants 9, no. 1: 16. https://doi.org/10.3390/antiox9010016

APA Style

Costa, F. P. d., Puty, B., Nogueira, L. S., Mitre, G. P., Santos, S. M. d., Teixeira, B. J. B., Kataoka, M. S. d. S., Martins, M. D., Barboza, C. A. G., Monteiro, M. C., Rogez, H., Oliveira, E. H. C. d., & Lima, R. R. (2020). Piceatannol Increases Antioxidant Defense and Reduces Cell Death in Human Periodontal Ligament Fibroblast under Oxidative Stress. Antioxidants, 9(1), 16. https://doi.org/10.3390/antiox9010016

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