Antioxidant Activity of Graptopetalum paraguayense E. Walther Leaf Extract Counteracts Oxidative Stress Induced by Ethanol and Carbon Tetrachloride Co-Induced Hepatotoxicity in Rats
Abstract
:1. Introduction
2. Materials and Methods
2.1. Preparation of GE50 Extract of G. Paraguayense
2.2. Animal Treatment
2.3. Serum and Liver Tissue Preparation
2.4. Determination of AST, ALT, LDH, and TAS Serum Levels in Rats
2.5. Measurement of MDA and GSH Levels, and GPx, SOD, and CAT, and GST Activities
2.6. Measurement of Antioxidants
2.7. Histopathology
2.8. Characterization of Phenolic Compounds in GE50 Extracts
2.9. Statistical Analysis
3. Results
3.1. Effects of GE50 on Body Weight Gain, Feed Efficiency, and Liver Index in Ethanol + CCl4 -Treated Group
3.2. Effects of GE50 on Ethanol + CCl4 -Induced Hepatotoxicity
3.3. Histological Analyses
3.4. Effects of GE50 on Hepatic MDA, Vitamin C, Vitamin E, and GSH Levels in Ethanol + CCl4-Treated Group
3.5. Effects of GE50 on Antioxidant Enzymatic Activities in Ethanol + CCl4-Treated Group
3.6. Polyphenolic Profile in GE50
4. Discussion
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Groups | Daily Body Weight Gain (g/Day/Rat) | Feed Efficiency (g Gain/g Feed) | Relative Liver Weight (g/100 g Body Weight) |
---|---|---|---|
Control | 5.58 ± 0.66 a | 0.21 ± 0.02 a | 2.96 ± 0.14 c |
GE50 | 5.92 ± 0.81 a | 0.21 ± 0.02 ab | 3.05 ± 0.19 bc |
Ethanol + CCl4 (EC) | 3.56 ± 0.66 c | 0.15 ± 0.01 c | 3.30 ± 0.11 a |
EC + GE50 | 4.68 ± 0.59 b | 0.20 ± 0.01 b | 3.10 ± 0.12 b |
EC + silymarin | 4.32 ± 0.74 b | 0.20 ± 0.02 ab | 3.17 ± 0.11 b |
Groups | AST (U/L) | ALT (U/L) | LDH (U/L) | TAS (nmole/L) |
---|---|---|---|---|
Control | 103.98 ± 14.0 c | 44.10 ± 5.4 c | 273.82 ± 94.9 c | 0.39 ± 0.1 a |
GE50 | 97.25 ± 19.8 c | 44.28 ± 3.8 c | 218.39 ± 35.2 c | 0.37 ± 0.1 a |
Ethanol + CCl4 (EC) | 184.40 ± 25.5 a | 66.89 ± 4.9 a | 563.04 ± 103.7 a | 0.15 ± 0.1 c |
EC + GE50 | 110.95 ± 14.0 bc | 50.01 ± 4.2 b | 403.20 ± 79.4 b | 0.28 ± 0.1 b |
EC + silymarin | 122.28 ± 24.2 b | 42.05 ± 6.6 c | 347.13 ± 43.9 b | 0.26 ± 0.1 b |
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Chao, W.-W.; Chen, S.-J.; Peng, H.-C.; Liao, J.-W.; Chou, S.-T. Antioxidant Activity of Graptopetalum paraguayense E. Walther Leaf Extract Counteracts Oxidative Stress Induced by Ethanol and Carbon Tetrachloride Co-Induced Hepatotoxicity in Rats. Antioxidants 2019, 8, 251. https://doi.org/10.3390/antiox8080251
Chao W-W, Chen S-J, Peng H-C, Liao J-W, Chou S-T. Antioxidant Activity of Graptopetalum paraguayense E. Walther Leaf Extract Counteracts Oxidative Stress Induced by Ethanol and Carbon Tetrachloride Co-Induced Hepatotoxicity in Rats. Antioxidants. 2019; 8(8):251. https://doi.org/10.3390/antiox8080251
Chicago/Turabian StyleChao, Wen-Wan, Shu-Ju Chen, Hui-Chen Peng, Jiunn-Wang Liao, and Su-Tze Chou. 2019. "Antioxidant Activity of Graptopetalum paraguayense E. Walther Leaf Extract Counteracts Oxidative Stress Induced by Ethanol and Carbon Tetrachloride Co-Induced Hepatotoxicity in Rats" Antioxidants 8, no. 8: 251. https://doi.org/10.3390/antiox8080251