Next Article in Journal
Mitochondria-Targeted Antioxidants for Treatment of Hearing Loss: A Systematic Review
Next Article in Special Issue
Propolis Extracts Inhibit UV-Induced Photodamage in Human Experimental In Vitro Skin Models
Previous Article in Journal
Antioxidants Special Issue: Peroxiredoxin 6 as a Unique Member of the Peroxiredoxin Family
Previous Article in Special Issue
The Role of Isothiocyanates as Cancer Chemo-Preventive, Chemo-Therapeutic and Anti-Melanoma Agents

Flavonoid Analysis and Antioxidant Activities of the Bryonia alba L. Aerial Parts

Department of Pharmaceutical Botany, Faculty of Pharmacy, “Iuliu Hat, ieganu” University of Medicine and Pharmacy, 400337 Cluj-Napoca, Romania
Laboratory of Pharmacognosy, Center of Interdisciplinary Research on Medicines, University of Liège, 4000 Liège, Belgium
Department of Pharmacognosy, Faculty of Pharmacy, “Iuliu Haţieganu” University of Medicine and Pharmacy, 400010 Cluj-Napoca, Romania
PlantExtrakt Ltd., Rădaia, 407059 Cluj-Napoca, Romania
Department of Pharmaceutical Industry, Faculty of Pharmacy, "Vasile Goldiş" Western University of Arad, 310045 Arad, Romania
Author to whom correspondence should be addressed.
Antioxidants 2019, 8(4), 108;
Received: 5 April 2019 / Revised: 16 April 2019 / Accepted: 17 April 2019 / Published: 20 April 2019
(This article belongs to the Special Issue Natural Products in Health Promotion and Disease Prevention)
Bryonia alba L. is the only Bryonia species found in Romanian flora, being known as a remedy for inflammatory pathologies or for its hepatoprotective and adaptogen activities. The present investigation studied the flavonoid composition and antioxidant activities of the aerial parts of this species. Flavonoid profile was evaluated by HPLC coupled with Diode Array Detection (DAD), while antioxidant capacity was assessed by various methods, testing different antioxidant mechanisms: DPPH (2,2-diphenyl-1-picrylhydrazyl), CUPRAC (cupric reducing antioxidant capacity), FRAP (ferric reducing ability of plasma), TEAC (Trolox equivalent antioxidant capacity), EPR (electron paramagnetic resonance method) and SNPAC (silver nanoparticles antioxidant capacity). Cytotoxicity was tested on human cancerous and healthy cell lines. Anti-plasmodial tests were performed on two strains of Plasmodium falciparum. Whole organism toxicity was assessed on zebrafish larvae. The HPLC-DAD analysis proved the presence of lutonarin, saponarin, isoorientin, and isovitexin as the major flavonoids in the composition of tested samples. Significant results were obtained for all antioxidant capacity assays. The cytotoxicity tests proved the absence of cellular and parasitic toxicity and these results were confirmed by the lack of toxicity on the zebrafish larvae model. This study proves a promising potential of the aerial parts of Bryonia alba L. as antioxidant agents. View Full-Text
Keywords: flavonoids; HPLC-DAD; antioxidant; toxicity flavonoids; HPLC-DAD; antioxidant; toxicity
MDPI and ACS Style

Ielciu, I.; Frédérich, M.; Hanganu, D.; Angenot, L.; Olah, N.-K.; Ledoux, A.; Crișan, G.; Păltinean, R. Flavonoid Analysis and Antioxidant Activities of the Bryonia alba L. Aerial Parts. Antioxidants 2019, 8, 108.

AMA Style

Ielciu I, Frédérich M, Hanganu D, Angenot L, Olah N-K, Ledoux A, Crișan G, Păltinean R. Flavonoid Analysis and Antioxidant Activities of the Bryonia alba L. Aerial Parts. Antioxidants. 2019; 8(4):108.

Chicago/Turabian Style

Ielciu, Irina, Michel Frédérich, Daniela Hanganu, Luc Angenot, Neli-Kinga Olah, Allison Ledoux, Gianina Crișan, and Ramona Păltinean. 2019. "Flavonoid Analysis and Antioxidant Activities of the Bryonia alba L. Aerial Parts" Antioxidants 8, no. 4: 108.

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

Back to TopTop