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Flavonoid Analysis and Antioxidant Activities of the Bryonia alba L. Aerial Parts

1
Department of Pharmaceutical Botany, Faculty of Pharmacy, “Iuliu Hat, ieganu” University of Medicine and Pharmacy, 400337 Cluj-Napoca, Romania
2
Laboratory of Pharmacognosy, Center of Interdisciplinary Research on Medicines, University of Liège, 4000 Liège, Belgium
3
Department of Pharmacognosy, Faculty of Pharmacy, “Iuliu Haţieganu” University of Medicine and Pharmacy, 400010 Cluj-Napoca, Romania
4
PlantExtrakt Ltd., Rădaia, 407059 Cluj-Napoca, Romania
5
Department of Pharmaceutical Industry, Faculty of Pharmacy, "Vasile Goldiş" Western University of Arad, 310045 Arad, Romania
*
Author to whom correspondence should be addressed.
Antioxidants 2019, 8(4), 108; https://doi.org/10.3390/antiox8040108
Received: 5 April 2019 / Revised: 16 April 2019 / Accepted: 17 April 2019 / Published: 20 April 2019
(This article belongs to the Special Issue Natural Products in Health Promotion and Disease Prevention)
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Abstract

Bryonia alba L. is the only Bryonia species found in Romanian flora, being known as a remedy for inflammatory pathologies or for its hepatoprotective and adaptogen activities. The present investigation studied the flavonoid composition and antioxidant activities of the aerial parts of this species. Flavonoid profile was evaluated by HPLC coupled with Diode Array Detection (DAD), while antioxidant capacity was assessed by various methods, testing different antioxidant mechanisms: DPPH (2,2-diphenyl-1-picrylhydrazyl), CUPRAC (cupric reducing antioxidant capacity), FRAP (ferric reducing ability of plasma), TEAC (Trolox equivalent antioxidant capacity), EPR (electron paramagnetic resonance method) and SNPAC (silver nanoparticles antioxidant capacity). Cytotoxicity was tested on human cancerous and healthy cell lines. Anti-plasmodial tests were performed on two strains of Plasmodium falciparum. Whole organism toxicity was assessed on zebrafish larvae. The HPLC-DAD analysis proved the presence of lutonarin, saponarin, isoorientin, and isovitexin as the major flavonoids in the composition of tested samples. Significant results were obtained for all antioxidant capacity assays. The cytotoxicity tests proved the absence of cellular and parasitic toxicity and these results were confirmed by the lack of toxicity on the zebrafish larvae model. This study proves a promising potential of the aerial parts of Bryonia alba L. as antioxidant agents. View Full-Text
Keywords: flavonoids; HPLC-DAD; antioxidant; toxicity flavonoids; HPLC-DAD; antioxidant; toxicity
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Ielciu, I.; Frédérich, M.; Hanganu, D.; Angenot, L.; Olah, N.-K.; Ledoux, A.; Crișan, G.; Păltinean, R. Flavonoid Analysis and Antioxidant Activities of the Bryonia alba L. Aerial Parts. Antioxidants 2019, 8, 108.

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