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Open AccessArticle

Characterization of Polyphenolic Compounds Extracted from Different Varieties of Almond Hulls (Prunus dulcis L.)

1
Department of Agriculture, Forest and Food Sciences (DISAFA), University of Turin, Largo Paolo Braccini, 2, 10095 Torino, Italy
2
Higher School of Food Industries of Tunis (ESIAT), University of Carthage, 1003, 58 Alain Savary, Tunisia
3
Department of Analytical Chemistry, Nutrition and Food Science, Faculty of Pharmacy, University of Santiago de Compostela, 15782 Santiago de Compostela, Spain
*
Authors to whom correspondence should be addressed.
Antioxidants 2019, 8(12), 647; https://doi.org/10.3390/antiox8120647
Received: 1 December 2019 / Revised: 11 December 2019 / Accepted: 13 December 2019 / Published: 16 December 2019
(This article belongs to the Special Issue Phenolic Profiling and Antioxidant Capacity in Plants)
Ultrasound-assisted extraction (UAE) was applied as a pretreatment technique to improve the recovery of polyphenols from the almond hulls of four Tunisian and three Italian almond varieties, followed by the characterization with HPLC-DAD. The operating parameters (solid/liquid ratio, extraction time, and ethanol concentrations) were optimized using a Response Surface Methodology. A polynomial equation was calculated to describe the relationship between the operating parameters and dependent variables as total polyphenolic content (TPC) and antioxidant activity (RSA). A desirability function approach was used to determine the optimum conditions for operating parameters: a solid:solvent ratio of 2 g/100 mL, an extraction time of 13 min, and an ethanol concentration of 51.2%. Among the almond varieties, Pizzuta and Fakhfekh showed the highest polyphenol content and antioxidant activity. HPLC-DAD analysis of almond hull extracts confirmed that chlorogenic acid, catechin, and protocatechuic acid were the most important polyphenols in almond hull. The results highlighted that UAE could be an effective technique for the recovery of phenolic compounds from almond hull, thereby making this byproduct a promising source of compounds with potential applications in food and healthcare sectors. View Full-Text
Keywords: almond hull; Prunus dulcis L.; ultrasound-assisted extraction; response surface methodology; radical scavenging activity; polyphenols almond hull; Prunus dulcis L.; ultrasound-assisted extraction; response surface methodology; radical scavenging activity; polyphenols
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Kahlaoui, M.; Borotto Dalla Vecchia, S.; Giovine, F.; Ben Haj Kbaier, H.; Bouzouita, N.; Barbosa Pereira, L.; Zeppa, G. Characterization of Polyphenolic Compounds Extracted from Different Varieties of Almond Hulls (Prunus dulcis L.). Antioxidants 2019, 8, 647.

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