Next Article in Journal
Methionine Sulfoxide Reductases of Archaea
Next Article in Special Issue
Cell-Type-Specific Modulation of Hydrogen Peroxide Cytotoxicity and 4-Hydroxynonenal Binding to Human Cellular Proteins In Vitro by Antioxidant Aloe vera Extract
Previous Article in Journal
The Functions of the Mammalian Methionine Sulfoxide Reductase System and Related Diseases
Previous Article in Special Issue
4-Hydroxynonenal in Redox Homeostasis of Gastrointestinal Mucosa: Implications for the Stomach in Health and Diseases
Article Menu
Issue 9 (September) cover image

Export Article

Open AccessArticle

Antioxidative 1,4-Dihydropyridine Derivatives Modulate Oxidative Stress and Growth of Human Osteoblast-Like Cells In Vitro

1
Laboratory for Oxidative Stress, Rudjer Boskovic Institute, Bijenicka 54, 10000 Zagreb, Croatia
2
Institute of Pathophysiology and Immunology, Medical University of Graz, A-8036 Graz, Austria
3
Latvian Institute of Organic Synthesis, 21 Aizkraukles Str., LV-1006 Riga, Latvia
*
Authors to whom correspondence should be addressed.
Antioxidants 2018, 7(9), 123; https://doi.org/10.3390/antiox7090123
Received: 16 July 2018 / Revised: 6 September 2018 / Accepted: 15 September 2018 / Published: 19 September 2018
(This article belongs to the Special Issue Antioxidants and Second Messengers of Free Radicals)
  |  
PDF [7353 KB, uploaded 21 September 2018]
  |  

Abstract

Oxidative stress has been implicated in pathophysiology of different human stress- and age-associated disorders, including osteoporosis for which antioxidants could be considered as therapeutic remedies as was suggested recently. The 1,4-dihydropyridine (DHP) derivatives are known for their pleiotropic activity, with some also acting as antioxidants. To find compounds with potential antioxidative activity, a group of 27 structurally diverse DHPs, as well as one pyridine compound, were studied. A group of 11 DHPs with 10-fold higher antioxidative potential than of uric acid, were further tested in cell model of human osteoblast-like cells. Short-term combined effects of DHPs and 50 µM H2O2 (1-h each), revealed better antioxidative potential of DHPs if administered before a stressor. Indirect 24-h effect of DHPs was evaluated in cells further exposed to mild oxidative stress conditions induced either by H2O2 or tert-butyl hydroperoxide (both 50 µM). Cell growth (viability and proliferation), generation of ROS and intracellular glutathione concentration were evaluated. The promotion of cell growth was highly dependent on the concentrations of DHPs used, type of stressor applied and treatment set-up. Thiocarbatone III-1, E2-134-1 III-4, Carbatone II-1, AV-153 IV-1, and Diethone I could be considered as therapeutic agents for osteoporosis although further research is needed to elucidate their bioactivity mechanisms, in particular in respect to signaling pathways involving 4-hydroxynoneal and related second messengers of free radicals. View Full-Text
Keywords: 1,4-dihydropyridine(s) (DHPs); oxidative stress; reactive oxygen species (ROS); antioxidant (AO); antioxidative activity (AOA); glutathione; cell viability and proliferation; 4-hydroxynonenal (4-HNE) 1,4-dihydropyridine(s) (DHPs); oxidative stress; reactive oxygen species (ROS); antioxidant (AO); antioxidative activity (AOA); glutathione; cell viability and proliferation; 4-hydroxynonenal (4-HNE)
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
SciFeed
Printed Edition Available!
A printed edition of this Special Issue is available here.

Share & Cite This Article

MDPI and ACS Style

Milkovic, L.; Vukovic, T.; Zarkovic, N.; Tatzber, F.; Bisenieks, E.; Kalme, Z.; Bruvere, I.; Ogle, Z.; Poikans, J.; Velena, A.; Duburs, G. Antioxidative 1,4-Dihydropyridine Derivatives Modulate Oxidative Stress and Growth of Human Osteoblast-Like Cells In Vitro. Antioxidants 2018, 7, 123.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Antioxidants EISSN 2076-3921 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top