Green Synthesized Zinc Oxide (ZnO) Nanoparticles Induce Oxidative Stress and DNA Damage in Lathyrus sativus L. Root Bioassay System

Panda, Kamal K.; Golari, Dambaru; Venugopal, A.; Achary, V. Mohan M.; Phaomei, Ganngam; Parinandi, Narasimham L.; Sahu, Hrushi K.; Panda, Brahma B.

doi:10.3390/antiox6020035

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Green Synthesized Zinc Oxide (ZnO) Nanoparticles Induce Oxidative Stress and DNA Damage in Lathyrus sativus L. Root Bioassay System

¹,

¹,

¹,

²,

³,

Narasimham L. Parinandi

⁴,

Hrushi K. Sahu

^5,† and

Brahma B. Panda

^1,*

Molecular Biology and Genomics Laboratory, Department of Botany, Berhampur University, Berhampur 760007, Odisha, India

Plant Molecular Biology Group, International Centre for Genetic Engineering and Biotechnology, New Delhi 110067, India

Material Chemistry Laboratory, Department of Chemistry, Berhampur University, Berhampur 760007, Odisha, India

⁴

Division of Pulmonary, Allergy, Critical Care, and Sleep Medicine, Department of Medicine, Davis Heart and Lung Research Institute, Ohio State University College of Medicine, Columbus, OH 43210, USA

⁵

Condensed Matter Physics Division, Indira Gandhi Centre for Atomic Research, Kalpakum, Tamil Nadu 603102, India

Author to whom correspondence should be addressed.

^†

Present address: C/92 Vidya Nilaya, Shanti Nagar, Goilundi, Berhampur 760004, India.

Academic Editor: Vytas Reipa

Antioxidants 2017, 6(2), 35; https://doi.org/10.3390/antiox6020035

Received: 15 March 2017 / Revised: 10 May 2017 / Accepted: 15 May 2017 / Published: 18 May 2017

(This article belongs to the Special Issue Nanomaterial Oxidative Stress)

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Abstract

Zinc oxide nanoparticles (ZnONP-GS) were synthesised from the precursor zinc acetate (Zn(CH₃COO)₂) through the green route using the milky latex from milk weed (Calotropis gigantea L. R. Br) by alkaline precipitation. Formation of the ZnONP-GS was monitored by UV-visible spectroscopy followed by characterization and confirmation by energy-dispersive X-ray spectroscopy (EDX), transmission electron microscopy (TEM), and X-ray diffraction (XRD). Both the ZnONP-GS and the commercially available ZnONP-S (Sigma-Aldrich) and cationic Zn²⁺ from Zn(CH₃COO)₂ were tested in a dose range of 0–100 mg·L⁻¹ for their potency (i) to induce oxidative stress as measured by the generation reactive oxygen species (ROS: O₂^•−, H₂O₂ and ^•OH), cell death, and lipid peroxidation; (ii) to modulate the activities of antioxidant enzymes: catalase (CAT), superoxide dismutase (SOD), guaiacol peroxidase (GPX), and ascorbate peroxidase (APX); and (iii) to cause DNA damage as determined by Comet assay in Lathyrus sativus L. root bioassay system. Antioxidants such as Tiron and dimethylthiourea significantly attenuated the ZnONP-induced oxidative and DNA damage, suggesting the involvement of ROS therein. Our study demonstrated that both ZnONP-GS and ZnONP-S induced oxidative stress and DNA damage to a similar extent but were significantly less potent than Zn²⁺ alone. View Full-Text

Keywords: zinc oxide nanoparticles; green synthesis; Calotropis gigantea; plant bioassay; Lathyrus sativus root; oxidative stress; comet assay; reactive oxygen species

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Panda, K.K.; Golari, D.; Venugopal, A.; Achary, V.M.M.; Phaomei, G.; Parinandi, N.L.; Sahu, H.K.; Panda, B.B. Green Synthesized Zinc Oxide (ZnO) Nanoparticles Induce Oxidative Stress and DNA Damage in Lathyrus sativus L. Root Bioassay System. Antioxidants 2017, 6, 35. https://doi.org/10.3390/antiox6020035

AMA Style

Panda KK, Golari D, Venugopal A, Achary VMM, Phaomei G, Parinandi NL, Sahu HK, Panda BB. Green Synthesized Zinc Oxide (ZnO) Nanoparticles Induce Oxidative Stress and DNA Damage in Lathyrus sativus L. Root Bioassay System. Antioxidants. 2017; 6(2):35. https://doi.org/10.3390/antiox6020035

Chicago/Turabian Style

Panda, Kamal K., Dambaru Golari, A. Venugopal, V. M.M. Achary, Ganngam Phaomei, Narasimham L. Parinandi, Hrushi K. Sahu, and Brahma B. Panda. 2017. "Green Synthesized Zinc Oxide (ZnO) Nanoparticles Induce Oxidative Stress and DNA Damage in Lathyrus sativus L. Root Bioassay System" Antioxidants 6, no. 2: 35. https://doi.org/10.3390/antiox6020035

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Green Synthesized Zinc Oxide (ZnO) Nanoparticles Induce Oxidative Stress and DNA Damage in Lathyrus sativus L. Root Bioassay System

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