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Antioxidants
Volume 12
Issue 4
10.3390/antiox12040930
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Article

Chemically Defined Lactobacillus plantarum Cell-Free Metabolites Demonstrate Cytoprotection in HepG2 Cells through Nrf2-Dependent Mechanism

by
Raja Rezgui
1,2,
Ruhi Walia
3,
Jyoti Sharma
3,
Dwinder Sidhu
3,
Khalid Alshagadali
1,2,
Saumya Ray Chaudhuri
4,
Amir Saeed
1,2,5,* and
Priyankar Dey
3,*
1
Department of Medical Laboratory Sciences, College of Applied Medical Sciences, University of Hail, Hail 55473, Saudi Arabia
2
Medical and Diagnostic Research Centre, University of Hail, Hail 55473, Saudi Arabia
3
Department of Biotechnology, Thapar Institute of Engineering and Technology, Patiala 147004, Punjab, India
4
Council of Scientific and Industrial Research (CSIR), Institute of Microbial Technology, Chandigarh 160036, India
5
Department of Medical Microbiology, Faculty of Medical Laboratory Sciences, University of Medical Sciences & Technology, Khartoum 12810, Sudan
*
Authors to whom correspondence should be addressed.
Antioxidants 2023, 12(4), 930; https://doi.org/10.3390/antiox12040930
Submission received: 16 March 2023 / Revised: 6 April 2023 / Accepted: 11 April 2023 / Published: 14 April 2023
(This article belongs to the Section Health Outcomes of Antioxidants and Oxidative Stress)
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Abstract

Centering around the concept that metabolites from the gut commensals can exert metabolic health benefits along the gut–liver axis, we tested whether the cell-free global metabolome of probiotic bacteria can exert hepatoprotective benefits against H2O2-induced oxidative stress. Cell-free global metabolites of Lactobacillus plantarum (LPM) were isolated and untargeted metabolomics was performed. The free radical scavenging potentials of LPM were measured. The cytoprotective effects of LPM were tested on HepG2 cells. A total of 66 diverse metabolites were identified in LPM, among which saturated fatty acids, amino acids and dicarboxylic acids were highly enriched. LPM attenuated cell damage, lipid peroxidation and the levels of intracellular cytoprotective enzymes in H2O2-treated cells. LPM also attenuated H2O2-induced increased expressions of TNF-α and IL-6. However, the cytoprotective effects of LPM were diminished in cells that were pretreated with a pharmacological inhibitor of Nrf2. Our data collectively indicate that LPM can significantly attenuate oxidative damage to HepG2 cells. However, the cytoprotective effects of LPM likely depend on an Nrf2-dependent mechanism.
Keywords: probiotic; Lactobacillus; antioxidant; Nrf2; metabolites; inflammation; oxidative stress; liver probiotic; Lactobacillus; antioxidant; Nrf2; metabolites; inflammation; oxidative stress; liver

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MDPI and ACS Style

Rezgui, R.; Walia, R.; Sharma, J.; Sidhu, D.; Alshagadali, K.; Ray Chaudhuri, S.; Saeed, A.; Dey, P. Chemically Defined Lactobacillus plantarum Cell-Free Metabolites Demonstrate Cytoprotection in HepG2 Cells through Nrf2-Dependent Mechanism. Antioxidants 2023, 12, 930. https://doi.org/10.3390/antiox12040930

AMA Style

Rezgui R, Walia R, Sharma J, Sidhu D, Alshagadali K, Ray Chaudhuri S, Saeed A, Dey P. Chemically Defined Lactobacillus plantarum Cell-Free Metabolites Demonstrate Cytoprotection in HepG2 Cells through Nrf2-Dependent Mechanism. Antioxidants. 2023; 12(4):930. https://doi.org/10.3390/antiox12040930

Chicago/Turabian Style

Rezgui, Raja, Ruhi Walia, Jyoti Sharma, Dwinder Sidhu, Khalid Alshagadali, Saumya Ray Chaudhuri, Amir Saeed, and Priyankar Dey. 2023. "Chemically Defined Lactobacillus plantarum Cell-Free Metabolites Demonstrate Cytoprotection in HepG2 Cells through Nrf2-Dependent Mechanism" Antioxidants 12, no. 4: 930. https://doi.org/10.3390/antiox12040930

APA Style

Rezgui, R., Walia, R., Sharma, J., Sidhu, D., Alshagadali, K., Ray Chaudhuri, S., Saeed, A., & Dey, P. (2023). Chemically Defined Lactobacillus plantarum Cell-Free Metabolites Demonstrate Cytoprotection in HepG2 Cells through Nrf2-Dependent Mechanism. Antioxidants, 12(4), 930. https://doi.org/10.3390/antiox12040930

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