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Open AccessArticle

Glutamatergic Fate of Neural Progenitor Cells of Rats with Inherited Audiogenic Epilepsy

Sechenov Institute of Evolutionary Physiology and Biochemistry of the Russian Academy of Sciences, 44 Thorez pr., 194223 St. Petersburg, Russia
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Brain Sci. 2020, 10(5), 311; https://doi.org/10.3390/brainsci10050311
Received: 30 April 2020 / Revised: 15 May 2020 / Accepted: 19 May 2020 / Published: 21 May 2020
(This article belongs to the Special Issue The Molecular and Cellular Mechanisms of Epilepsy)
Epilepsy is associated with aberrant neurogenesis in the hippocampus and may underlie the development of hereditary epilepsy. In the present study, we analyzed the differentiation fate of neural progenitor cells (NPC), which were isolated from the hippocampus of embryos of Krushinsky-Molodkina (KM) rats genetically prone to audiogenic epilepsy. NPCs from embryos of Wistar rats were used as the control. We found principal differences between Wistar and KM NPC in unstimulated controls: Wistar NPC culture contained both gamma-aminobutyric acid (GABA) and glutamatergic neurons; KM NPC culture was mainly represented by glutamatergic cells. The stimulation of glutamatergic differentiation of Wistar NPC resulted in a significant increase in glutamatergic cell number that was accompanied by the activation of protein kinase A. The stimulation of KM NPC led to a decrease in immature glutamatergic cell number and was associated with the activation of extracellular signal-regulated kinases 1 and 2 (ERK1/2) and protein kinase B/ glycogen synthase kinase 3 beta (Akt/GSK3β), which indicates the activation of glutamatergic cell maturation. These results suggest genetically programmed abnormalities in KM rats that determine the glutamatergic fate of NPC and contribute to the development of audiogenic epilepsy.
Keywords: Krushinsky-Molodkina rats; hippocampus; neuronal differentiation; PKA; ERK1/2; Akt; GSK3β Krushinsky-Molodkina rats; hippocampus; neuronal differentiation; PKA; ERK1/2; Akt; GSK3β
MDPI and ACS Style

Naumova, A.A.; Oleynik, E.A.; Chernigovskaya, E.V.; Glazova, M.V. Glutamatergic Fate of Neural Progenitor Cells of Rats with Inherited Audiogenic Epilepsy. Brain Sci. 2020, 10, 311.

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