Optical Properties of Buffers and Cell Culture Media for Optofluidic and Sensing Applications
by
Van Thuy Hoang 1,2, Grzegorz Stępniewski 1,2, Karolina H. Czarnecka 3,4
, Rafał Kasztelanic 1,2, Van Cao Long 5, Khoa Dinh Xuan 6, Liyang Shao 7, Mateusz Śmietana 3,7,* and Ryszard Buczyński 1,2,*
Van Thuy Hoang 1,2, Grzegorz Stępniewski 1,2, Karolina H. Czarnecka 3,4, Rafał Kasztelanic 1,2, Van Cao Long 5, Khoa Dinh Xuan 6, Liyang Shao 7, Mateusz Śmietana 3,7,* and Ryszard Buczyński 1,2,*
1
Faculty of Physics, University of Warsaw, Pasteura 7, 02-093 Warsaw, Poland
2
Institute of Electronic Materials Technology, Wólczyńska 133, 01-919 Warsaw, Poland
3
Institute of Microelectronics and Optoelectronics, Warsaw University of Technology, Koszykowa 75, 00-662 Warsaw, Poland
4
Department of Biomedicine and Genetics, Medical University of Lodz, Pomorska 251, 92-213 Lodz, Poland
5
Institute of Physics, University of Zielona Gora, Prof. Szafrana 4a, 65-516 Zielona Góra, Poland
6
Department of Physics, Vinh University, Le Duan 182, Vinh, Nghe An 420000, Vietnam
7
Department of Electrical and Electronic Engineering, Southern University of Science and Technology, Shenzhen 518055, China
*
Authors to whom correspondence should be addressed.
Appl. Sci. 2019, 9(6), 1145; https://doi.org/10.3390/app9061145
Received: 28 December 2018 / Revised: 19 February 2019 / Accepted: 12 March 2019 / Published: 18 March 2019
(This article belongs to the Special Issue Recent Progress in Fiber Optic Sensors: Bringing Light to Measurement)
Interactions between light and various cells in cultures, such as bacteria or mammalian cells, are widely applied for optical sensors and optofluidic systems. These microorganisms need to be kept in proper aqueous media, referred to as buffers or cell culture media, that are required, respectively, for stable storage or delivering biochemical nutrients for their growth. When experiments or numerical analyses on optical devices are performed, the properties of these media are usually considered to be similar to those of pure water, with negligible influence of biochemical compounds on the medium’s optical properties. In this work, we investigated the transmission, material dispersion, and scattering properties of selected and widely used buffers and cell culture media. We show that the optical properties of these media may significantly vary from those of water. Well-defined properties of buffers and cell culture media are essential for proper design of various optical sensing or future optofluidic systems dealing with biological structures.
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Keywords:
optical properties; cell culture media; optofluidics; optical sensing; label-free biosensing
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MDPI and ACS Style
Hoang, V.T.; Stępniewski, G.; Czarnecka, K.H.; Kasztelanic, R.; Long, V.C.; Xuan, K.D.; Shao, L.; Śmietana, M.; Buczyński, R. Optical Properties of Buffers and Cell Culture Media for Optofluidic and Sensing Applications. Appl. Sci. 2019, 9, 1145.
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