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Pump-Probe Time-Resolved Serial Femtosecond Crystallography at SACLA: Current Status and Data Collection Strategies

by 1,2,*, 3,*, 2,4 and 1,2
1
Department of Cell Biology, Graduate School of Medicine, Kyoto University, Yoshidakonoe-cho, Sakyo-ku, Kyoto 606-8501, Japan
2
RIKEN SPring-8 Center, 1-1-1, Kouto, Sayo-cho, Sayo-gun, Hyogo 679-5148, Japan
3
Graduate School of Life Science, University of Hyogo, Kouto 3-2-1, Kamigori-cho, Ako-gun, Hyogo 678-1297, Japan
4
Japan Synchrotron Radiation Research Institute, 1-1-1 Kouto, Sayo, Hyogo 679-5198, Japan
*
Authors to whom correspondence should be addressed.
Appl. Sci. 2019, 9(24), 5505; https://doi.org/10.3390/app9245505
Received: 12 November 2019 / Revised: 10 December 2019 / Accepted: 12 December 2019 / Published: 14 December 2019
(This article belongs to the Special Issue Science at X-ray Free Electron Lasers)
Structural information on protein dynamics is a critical factor in fully understanding the protein functions. Pump-probe time-resolved serial femtosecond crystallography (TR-SFX) is a recently established technique for visualizing the structural changes or reactions in proteins that are at work with high spatial and temporal resolution. In the pump-probe method, protein microcrystals are continuously delivered from an injector and exposed to an X-ray free-electron laser (XFEL) pulse after a trigger to initiate a reaction, such as light, chemicals, temperature, and electric field, which affords the structural snapshots of intermediates that occur in the protein. We are in the process of developing the device and techniques for pump-probe TR-SFX while using XFEL produced at SPring-8 Angstrom Compact Free-Electron Laser (SACLA). In this paper, we described our current development details and data collection strategies for the optical pump X-ray probe TR-SFX experiment at SACLA and then reported the techniques of in crystallo TR spectroscopy, which is useful in clarifying the nature of reaction that takes place in crystals in advance. View Full-Text
Keywords: X-ray free-electron laser; time-resolved crystallography; pump-probe experiment; microcrystals X-ray free-electron laser; time-resolved crystallography; pump-probe experiment; microcrystals
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MDPI and ACS Style

Nango, E.; Kubo, M.; Tono, K.; Iwata, S. Pump-Probe Time-Resolved Serial Femtosecond Crystallography at SACLA: Current Status and Data Collection Strategies. Appl. Sci. 2019, 9, 5505. https://doi.org/10.3390/app9245505

AMA Style

Nango E, Kubo M, Tono K, Iwata S. Pump-Probe Time-Resolved Serial Femtosecond Crystallography at SACLA: Current Status and Data Collection Strategies. Applied Sciences. 2019; 9(24):5505. https://doi.org/10.3390/app9245505

Chicago/Turabian Style

Nango, Eriko, Minoru Kubo, Kensuke Tono, and So Iwata. 2019. "Pump-Probe Time-Resolved Serial Femtosecond Crystallography at SACLA: Current Status and Data Collection Strategies" Applied Sciences 9, no. 24: 5505. https://doi.org/10.3390/app9245505

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