Next Article in Journal
A Novel Massive Deployment Solution Based on the Peer-to-Peer Protocol
Next Article in Special Issue
The Characterisation and Quantification of Immobilised Concanavalin A on Quartz Surfaces Based on The Competitive Binding to Glucose and Fluorescent Labelled Dextran
Previous Article in Journal
Improved Defect Detection Using Adaptive Leaky NLMS Filter in Guided-Wave Testing of Pipelines
Previous Article in Special Issue
A Review on Nanoparticles as Boon for Biogas Producers—Nano Fuels and Biosensing Monitoring
Open AccessArticle

Development of an Electrochemical Biosensor for Rapid and Effective Detection of Pathogenic Escherichia coli in Licorice Extract

1
College of Pharmaceutical Engineering of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
2
Tasly Pharmaceutical Group Co., Ltd., Tianjin 300410, China
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Appl. Sci. 2019, 9(2), 295; https://doi.org/10.3390/app9020295
Received: 30 November 2018 / Revised: 9 January 2019 / Accepted: 11 January 2019 / Published: 15 January 2019
(This article belongs to the Special Issue Nano-Biointerface for Biosensing)
An aptamer-based electrochemical biosensor was successfully developed and applied in the rapid detection of pathogenic Escherichia coli (E. coli) in licorice extract. The thiolated capture probes were firstly immobilized on a gold electrode, and then the biotinylated aptamer probes for E. coli were introduced by hybridization with the capture probes. Due to the stronger interaction between the aptamer and the E. coli, a part of the biotinylated aptamers will dissociate from the capture probes in the presence of E. coli. The residual biotinylated aptamer probes can quantitatively bind with streptavidin-alkaline phosphatase. Subsequently, α-naphthyl phosphate substrate was catalytically hydrolyzed to generate electrochemical response, which could be recorded by a differential pulse voltammetry. The dependence of the peak current on the logarithm of E. coli concentration in the range from 5.0 × 102 colony forming units (CFU)/mL to 5.0 × 107 CFU/mL exhibited a linear trend with a detection limit of 80 CFU/mL. The relative standard deviation of 5 successive scans was 5.3%, 4.5% and 1.1% for 5.0 × 102, 5.0 × 105 and 5.0 × 107 CFU/mL E. coli, respectively. In the detection of the licorice extract samples, the results obtained from the proposed strategy and traditional culture counting method were close to each other, but the time consumption was only ~1/30 compared with the traditional method. These results demonstrate that the designed biosensor can be potentially utilized for rapid microbial examination in traditional Chinese medicine and relevant fields. View Full-Text
Keywords: aptamer; electrochemical; Escherichia coli; biosensor; licorice aptamer; electrochemical; Escherichia coli; biosensor; licorice
Show Figures

Graphical abstract

MDPI and ACS Style

Wang, H.; Zhao, Y.; Bie, S.; Suo, T.; Jia, G.; Liu, B.; Ye, R.; Li, Z. Development of an Electrochemical Biosensor for Rapid and Effective Detection of Pathogenic Escherichia coli in Licorice Extract. Appl. Sci. 2019, 9, 295. https://doi.org/10.3390/app9020295

AMA Style

Wang H, Zhao Y, Bie S, Suo T, Jia G, Liu B, Ye R, Li Z. Development of an Electrochemical Biosensor for Rapid and Effective Detection of Pathogenic Escherichia coli in Licorice Extract. Applied Sciences. 2019; 9(2):295. https://doi.org/10.3390/app9020295

Chicago/Turabian Style

Wang, Haixia; Zhao, Yuwen; Bie, Songtao; Suo, Tongchuan; Jia, Guangcheng; Liu, Boshi; Ye, Ruiping; Li, Zheng. 2019. "Development of an Electrochemical Biosensor for Rapid and Effective Detection of Pathogenic Escherichia coli in Licorice Extract" Appl. Sci. 9, no. 2: 295. https://doi.org/10.3390/app9020295

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop