3D Bioprinting Human Induced Pluripotent Stem Cell-Derived Neural Tissues Using a Novel Lab-on-a-Printer Technology
by
Laura De la Vega 1, Diego A. Rosas Gómez 2, Emily Abelseth 3, Laila Abelseth 3, Victor Allisson da Silva 4 and Stephanie M. Willerth 1,3,5,*
Laura De la Vega 1, Diego A. Rosas Gómez 2, Emily Abelseth 3, Laila Abelseth 3, Victor Allisson da Silva 4 and Stephanie M. Willerth 1,3,5,*
1
Department of Mechanical Engineering, University of Victoria, BC V8P 5C2, Canada
2
School of Engineering and Sciences, Monterrey Institute of Technology and Higher Education, Mexico City 14380, Mexico
3
Biomedical Engineering Program, University of Victoria, BC V8P 5C2, Canada
4
Center of Mathematics, Computing and Cognition, Federal University of ABC, São Paulo 09606-045, Brazil
5
Division of Medical Sciences, University of Victoria, BC V8P 5C2, Canada
*
Author to whom correspondence should be addressed.
Appl. Sci. 2018, 8(12), 2414; https://doi.org/10.3390/app8122414
Received: 29 October 2018 / Revised: 16 November 2018 / Accepted: 23 November 2018 / Published: 28 November 2018
(This article belongs to the Special Issue Biomaterials and Scaffolds in Tissue Engineering Applications and Cancer Therapies 2018)
Most neurological diseases and disorders lack true cures, including spinal cord injury (SCI). Accordingly, current treatments only alleviate the symptoms of these neurological diseases and disorders. Engineered neural tissues derived from human induced pluripotent stem cells (hiPSCs) can serve as powerful tools to identify drug targets for treating such diseases and disorders. In this work, we demonstrate how hiPSC-derived neural progenitor cells (NPCs) can be bioprinted into defined structures using Aspect Biosystems’ novel RX1 bioprinter in combination with our unique fibrin-based bioink in rapid fashion as it takes under 5 min to print four tissues. This printing process preserves high levels of cell viability (>81%) and their differentiation capacity in comparison to less sophisticated bioprinting methods. These bioprinted neural tissues expressed the neuronal marker, βT-III (45 ± 20.9%), after 15 days of culture and markers associated with spinal cord (SC) motor neurons (MNs), such as Olig2 (68.8 ± 6.9%), and HB9 (99.6 ± 0.4%) as indicated by flow cytometry. The bioprinted neural tissues expressed the mature MN marker, ChaT, after 30 days of culture as indicated by immunocytochemistry. In conclusion, we have presented a novel method for high throughput production of mature hiPSC-derived neural tissues with defined structures that resemble those found in the SC.
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Keywords:
3D bioprinting; neural tissue; motor neurons; pluripotent stem cells; biomaterials; spinal cord injury; lab on a printer; fibrin
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
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MDPI and ACS Style
De la Vega, L.; A. Rosas Gómez, D.; Abelseth, E.; Abelseth, L.; Allisson da Silva, V.; Willerth, S.M. 3D Bioprinting Human Induced Pluripotent Stem Cell-Derived Neural Tissues Using a Novel Lab-on-a-Printer Technology. Appl. Sci. 2018, 8, 2414.
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