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Open AccessArticle

Determination of Trolox Equivalent Antioxidant Capacity in Berries Using Amperometric Tyrosinase Biosensor Based on Multi-Walled Carbon Nanotubes

1
Department of Chemistry, Faculty of Mathematics and Natural Sciences, University of Prishtina, Str. Mother Teresa, 10000 Prishtina, Kosovo
2
Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic
3
Central European Institute of Technology, Brno University of Technology, Purkynova 123, CZ-612 00 Brno, Czech Republic
4
CEITEC—Central European Institute of Technology, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic
5
Department of Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice, Studentska 573, 53210 Pardubice, Czech Republic
6
Joint Laboratory of Solid State Chemistry of Institute of Macromolecular Chemistry of the Academy of Sciences of the Czech Republic and the University of Pardubice, 532 10 Pardubice, Czech Republic
*
Author to whom correspondence should be addressed.
Appl. Sci. 2020, 10(7), 2497; https://doi.org/10.3390/app10072497
Received: 12 February 2020 / Revised: 29 March 2020 / Accepted: 1 April 2020 / Published: 5 April 2020
(This article belongs to the Special Issue Applications of Enzymatic Electrochemical Biosensors)
In this contribution, Trolox equivalent antioxidant capacity (TEAC) was determined in various berries using carbon paste tyrosinase biosensor with multi-walled carbon nanotubes (MWCNTs), coated with Nafion® layer. Electrochemical behaviour of the biosensor and influence of MWCNTs on carbon paste surface were studied with respect to the sensitive amperometric detection of total content of phenolic compounds in berries, expressed as concentration equivalent of Trolox. After optimization of key instrumental and electroanalytical parameters, the biosensor was used for determination of TEAC in blackberries, blueberries, cranberries, raspberries and strawberries by method of multiple standard additions. Electrochemical TEAC assays corresponded well with results obtained by spectrophotometric 1,1-diphenyl-2-picrylhydrazyl radical method, known as DPPH assay. Obtained values were compared with those listed in the National Nutrient Database for additional antioxidant capacity assays as well. View Full-Text
Keywords: amperometry; ascorbic acid; berries; biosensor; multi-wall carbon nanotubes; carbon paste electrode; tyrosinase; Trolox; antioxidant capacity amperometry; ascorbic acid; berries; biosensor; multi-wall carbon nanotubes; carbon paste electrode; tyrosinase; Trolox; antioxidant capacity
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MDPI and ACS Style

Frangu, A.; Ashrafi, A.M.; Sýs, M.; Arbneshi, T.; Metelka, R.; Adam, V.; Vlček, M.; Richtera, L. Determination of Trolox Equivalent Antioxidant Capacity in Berries Using Amperometric Tyrosinase Biosensor Based on Multi-Walled Carbon Nanotubes. Appl. Sci. 2020, 10, 2497. https://doi.org/10.3390/app10072497

AMA Style

Frangu A, Ashrafi AM, Sýs M, Arbneshi T, Metelka R, Adam V, Vlček M, Richtera L. Determination of Trolox Equivalent Antioxidant Capacity in Berries Using Amperometric Tyrosinase Biosensor Based on Multi-Walled Carbon Nanotubes. Applied Sciences. 2020; 10(7):2497. https://doi.org/10.3390/app10072497

Chicago/Turabian Style

Frangu, Arbër; Ashrafi, Amir M.; Sýs, Milan; Arbneshi, Tahir; Metelka, Radovan; Adam, Vojtěch; Vlček, Milan; Richtera, Lukáš. 2020. "Determination of Trolox Equivalent Antioxidant Capacity in Berries Using Amperometric Tyrosinase Biosensor Based on Multi-Walled Carbon Nanotubes" Appl. Sci. 10, no. 7: 2497. https://doi.org/10.3390/app10072497

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