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Article

A Comprehensive HPLC-DAD-ESI-MS Validated Method for the Quantification of 16 Phytoestrogens in Food, Serum and Urine

1
Biomolecular Medicine, Department of Metabolism, Division of Systems Medicine, Digestion, and Reproduction, Imperial College London, London SW7 2AZ, UK
2
Department of Food Science, Centro de Investigación en Alimentación y Desarrollo, A.C. Hermosillo 83004, Mexico
3
Department of Nutrition, Centro de Investigación en Alimentación y Desarrollo, A.C. Hermosillo 83004, Mexico
*
Author to whom correspondence should be addressed.
Appl. Sci. 2020, 10(22), 8147; https://doi.org/10.3390/app10228147
Received: 8 October 2020 / Revised: 3 November 2020 / Accepted: 12 November 2020 / Published: 17 November 2020
(This article belongs to the Special Issue Analysis of Chemical Biomarkers and Contaminants in Food)
There has been increased interest in phytoestrogens due to their potential effect in reducing the risk of developing cancer and cardiovascular disease. To evaluate phytoestrogens’ exposure, sensitive and accurate methods should be developed for their quantification in food and human matrices. The present study aimed to validate a comprehensive liquid chromatography-mass spectrometry (LC-MS) method for the quantification of 16 phytoestrogens: Biochanin A, secoisolariciresinol, matairesinol, enterodiol, enterolactone, equol, quercetin, genistein, glycitein, luteolin, naringenin, kaempferol, formononetin, daidzein, resveratrol and coumestrol, in food, serum and urine. Phytoestrogen extraction was performed by solid-phase extraction (food and serum) and liquid-liquid extraction (urine), and analyzed by LC diode-array detector (DAD) coupled with a single quadrupole MS with electrospray ionization (ESI) in negative mode. Validation included selectivity, sensibility, recovery, accuracy and precision. The method was proved to be specific, with a linear response (r2 ≥ 0.97). Limits of quantification were 0.008–3.541 ng/mL for food, 0.01–1.77 ng/mL for serum and 0.003–0.251 ng/mL for urine. Recoveries were 66–113% for food, 63–104% for serum and 76–111% for urine. Accuracy and precision were below 15% (except for enterodiol in food with 18% and resveratrol in urine with 15.71%). The method is suitable for the quantification of a wide number of phytoestrogens in food, serum and urine. The method was successfully applied in highly consumed food items (n = 6) from North Mexico and biofluids from healthy women (n = 10). View Full-Text
Keywords: phytoestrogens; food metabolites; biofluids; flavonoids; lignans; coumestrol; resveratrol phytoestrogens; food metabolites; biofluids; flavonoids; lignans; coumestrol; resveratrol
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MDPI and ACS Style

Palma-Duran, S.A.; Caire-Juvera, G.; Campa-Siqueiros, M.M.; Chávez-Suárez, K.M.; Robles-Burgueño, M.d.R.; Gutiérrez-Coronado, M.L.; Bermúdez-Almada, M.d.C.; Saucedo-Tamayo, M.d.S.; Grajeda-Cota, P.; Valenzuela-Quintanar, A.I. A Comprehensive HPLC-DAD-ESI-MS Validated Method for the Quantification of 16 Phytoestrogens in Food, Serum and Urine. Appl. Sci. 2020, 10, 8147. https://doi.org/10.3390/app10228147

AMA Style

Palma-Duran SA, Caire-Juvera G, Campa-Siqueiros MM, Chávez-Suárez KM, Robles-Burgueño MdR, Gutiérrez-Coronado ML, Bermúdez-Almada MdC, Saucedo-Tamayo MdS, Grajeda-Cota P, Valenzuela-Quintanar AI. A Comprehensive HPLC-DAD-ESI-MS Validated Method for the Quantification of 16 Phytoestrogens in Food, Serum and Urine. Applied Sciences. 2020; 10(22):8147. https://doi.org/10.3390/app10228147

Chicago/Turabian Style

Palma-Duran, Susana A., Graciela Caire-Juvera, Melissa M. Campa-Siqueiros, Karina M. Chávez-Suárez, María d.R. Robles-Burgueño, María L. Gutiérrez-Coronado, María d.C. Bermúdez-Almada, María d.S. Saucedo-Tamayo, Patricia Grajeda-Cota, and Ana I. Valenzuela-Quintanar 2020. "A Comprehensive HPLC-DAD-ESI-MS Validated Method for the Quantification of 16 Phytoestrogens in Food, Serum and Urine" Applied Sciences 10, no. 22: 8147. https://doi.org/10.3390/app10228147

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