Next Article in Journal
The Circadian Effect Versus Mesopic Vision Effect in Road Lighting Applications
Next Article in Special Issue
Biomimetic Amorphous Titania Nanoparticles as Ultrasound Responding Agents to Improve Cavitation and ROS Production for Sonodynamic Therapy
Previous Article in Journal
Raman Spectroscopy as Noninvasive Method of Diagnosis of Pediatric Onset Inflammatory Bowel Disease
Article

Microbial Mediated Synthesis of Silver Nanoparticles by Lactobacillus Plantarum TA4 and its Antibacterial and Antioxidant Activity

1
Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia
2
Bioprocessing and Biomanufacturing Research Centre, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia
3
Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia
*
Author to whom correspondence should be addressed.
Appl. Sci. 2020, 10(19), 6973; https://doi.org/10.3390/app10196973
Received: 18 August 2020 / Revised: 12 September 2020 / Accepted: 15 September 2020 / Published: 5 October 2020
(This article belongs to the Special Issue Applications of Green Nanomaterials in Biomedical Treatment)
The present study aimed to investigate the ability of Lactobacillus plantarum TA4 in tolerating Ag+ and its ability to produce silver nanoparticles (AgNPs). The biosynthesized AgNPs were characterized using UV–Visible spectroscopy (UV–Vis), dynamic light scattering (DLS), Fourier-transform infrared (FTIR), and high-resolution transmission electron microscope (HR-TEM). The cell biomass of L. plantarum TA4 demonstrated the ability to tolerate Ag+ at a concentration of 2 mM, followed by the formation of AgNPs. This was confirmed by the visual observation of color changes and a presence of maximum UV–Vis absorption centered at 429 nm. HR-TEM analysis revealed that the AgNPs were spherical with an average size of 14.0 ± 4.7 nm, while the SEM-EDX analysis detected that the particles were primarily located on the cell membrane of L. plantarum TA4. Further, DLS analysis revealed that the polydispersity index (PDI) value of biosynthesized AgNPs was 0.193, implying the monodispersed characteristic of NPs. Meanwhile, the FTIR study confirmed the involvement of functional groups from the cell biomass that involved in the reduction process. Moreover, biosynthesized AgNPs exhibited antibacterial activity against Gram-positive and Gram-negative pathogens in a concentration-dependent manner. Furthermore, the antioxidant property of biosynthesized AgNPs that was evaluated using the DPPH assay showed considerable antioxidant potential. Results from this study provide a sustainable and inexpensive method for the production of AgNPs. View Full-Text
Keywords: antibacterial; biosynthesis; FTIR; HR-TEM; Lactobacillus; silver nanoparticles; sustainable antibacterial; biosynthesis; FTIR; HR-TEM; Lactobacillus; silver nanoparticles; sustainable
Show Figures

Figure 1

MDPI and ACS Style

Mohd Yusof, H.; Abdul Rahman, N.; Mohamad, R.; Zaidan, U.H. Microbial Mediated Synthesis of Silver Nanoparticles by Lactobacillus Plantarum TA4 and its Antibacterial and Antioxidant Activity. Appl. Sci. 2020, 10, 6973. https://doi.org/10.3390/app10196973

AMA Style

Mohd Yusof H, Abdul Rahman N, Mohamad R, Zaidan UH. Microbial Mediated Synthesis of Silver Nanoparticles by Lactobacillus Plantarum TA4 and its Antibacterial and Antioxidant Activity. Applied Sciences. 2020; 10(19):6973. https://doi.org/10.3390/app10196973

Chicago/Turabian Style

Mohd Yusof, Hidayat, Nor’Aini Abdul Rahman, Rosfarizan Mohamad, and Uswatun H. Zaidan 2020. "Microbial Mediated Synthesis of Silver Nanoparticles by Lactobacillus Plantarum TA4 and its Antibacterial and Antioxidant Activity" Applied Sciences 10, no. 19: 6973. https://doi.org/10.3390/app10196973

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop