Open AccessArticle
In Vitro Efficient Expansion of Tumor Cells Deriving from Different Types of Human Tumor Samples
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Ilaria Turin, Roberta Schiavo, Marcello Maestri, Ombretta Luinetti, Barbara Dal Bello, Marco Paulli, Paolo Dionigi, Marianna Roccio, Arsenio Spinillo, Federica Ferulli, Matteo Tanzi, Rita Maccario, Daniela Montagna and Paolo Pedrazzoli
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Abstract
Obtaining human tumor cell lines from fresh tumors is essential to advance our understanding of antitumor immune surveillance mechanisms and to develop new
ex vivo strategies to generate an efficient anti-tumor response. The present study delineates a simple and rapid method for efficiently
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Obtaining human tumor cell lines from fresh tumors is essential to advance our understanding of antitumor immune surveillance mechanisms and to develop new
ex vivo strategies to generate an efficient anti-tumor response. The present study delineates a simple and rapid method for efficiently establishing primary cultures starting from tumor samples of different types, while maintaining the immuno-histochemical characteristics of the original tumor. We compared two different strategies to disaggregate tumor specimens. After short or long term
in vitro expansion, cells analyzed for the presence of malignant cells demonstrated their neoplastic origin. Considering that tumor cells may be isolated in a closed system with high efficiency, we propose this methodology for the
ex vivo expansion of tumor cells to be used to evaluate suitable new drugs or to generate tumor-specific cytotoxic T lymphocytes or vaccines.
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