Physiological Parameters to Identify Suitable Blood Donor Cows for Preparation of Platelet Rich Plasma
by
, , , , and
Anna Lange-Consiglio
1,*
,
Rosangela Garlappi
2,
Chiara Spelta
3,
Antonella Idda
1,
Stefano Comazzi
1,
Rita Rizzi
1 and
Fausto Cremonesi
1 1
Department of Veterinary Medicine, Università degli Studi di Milano, 26900 Lodi, Italy
2
Independent Researcher, 20133 Milan, Italy
3
Independent Researcher, 27100 Pavia, Italy
*
Author to whom correspondence should be addressed.
Animals 2021, 11(8), 2296; https://doi.org/10.3390/ani11082296
Submission received: 24 June 2021
/
Revised: 30 July 2021
/
Accepted: 30 July 2021
/
Published: 4 August 2021
(This article belongs to the Special Issue Mesenchymal Stromal Cells and Platelet Rich Plasma: Their Regenerative Potential in Reproductive Disorders in Farm Animals)
Simple Summary
Platelet rich plasma is a biological product obtained from blood and used for regenerative treatments of different pathologies. It is characterized by a high concentration of platelets (at least 3 times the physiological level) containing many growth factors with anti-inflammatory, bactericidal and regenerative properties. In human medicine, PRP is used in an autologous way, it means that the blood donor is also the recipient. In veterinary medicine, PRP is used to treat different diseases or lesions and in bovine species to treat mastitis. In this context, the opportunity to have PRP ready to use, stored from donor cows of the same farm where it will be used, would be very useful in treating this pathology immediately when it occurs. For this purpose, the present research aimed to detect parameters useful to identify the most suitable cows to be used as blood donors to obtain the highest yield of PRP (milliliters of PRP obtained with respect to milliliters of initial blood). Our results showed that blood collection from the mammary vein within three months of parturition, from nonpregnant cows at 5 years of age, but not the blood collection season, were associated with a high yield of PRP.
Abstract
Platelet rich plasma (PRP) has been shown to be beneficial in the treatment of bovine mastitis, with an action comparable to that of antibiotics. Autologous treatment is feasible in experimental conditions but is difficult to apply in field conditions, particularly in acute mastitis. The ideal scenario would be to have heterologous PRP stored on every farm so that it is readily available when needed. In this paper, we analysed data collected during bovine mastitis treatment with heterologous PRP produced by casual donor cows on several farms. We tried to identify parameters which might be useful to identify the most suitable cows to be used as blood donors, to obtain the highest yield of PRP. Variables considered for each animal were the age, the parity, the date of the last parturition, the season of blood collection, the site of blood collection (jugular or mammary vein) and the reproductive status e.g., pregnant or not pregnant. There were statistically significant differences for all the variables considered from the 135 blood cows, except for the blood collection season. The highest yield of PRP was associated with nonpregnancy blood collection within three months of parturition, parity 3 or 4, and blood collection from the mammary vein.
Keywords:
cow; blood donor; mastitis; PRP; yield
