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Article

Changes in Expression of Complement Components in the Ovine Spleen during Early Pregnancy

School of Life Sciences and Food Engineering, Hebei University of Engineering, Handan 056038, China
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Author to whom correspondence should be addressed.
Academic Editor: Rosa Maria L.N. Pereira
Animals 2021, 11(11), 3183; https://doi.org/10.3390/ani11113183
Received: 24 September 2021 / Revised: 2 November 2021 / Accepted: 4 November 2021 / Published: 8 November 2021
(This article belongs to the Special Issue Advances in Animal Fertility Preservation)
Complement regulation is related to fetal growth during early pregnancy in humans. The results show that C1q increased during early pregnancy, and C1r, C1s, C2, C3 and C5b enhanced at day 25 of gestation, and C4a and C9 increased on days 13 and 16 of pregnancy in the ovine maternal spleen. In summary, a complement pathway participates in maternal immune regulation during early gestation.
During early gestation in humans, complement regulation is essential for normal fetal growth. It is supposed that a complement pathway participates in maternal splenic immune regulation at the early stage of gestation in ewes. The aim of this study was to analyze the effects of early pregnancy on the expression of complement components in the maternal spleen of ewes. In this study, ovine spleens were sampled on day 16 of nonpregnancy, and days 13, 16 and 25 of gestation. RT-qPCR, Western blot and immunohistochemical analysis were used to detect the changes in expression of complement components in the ovine maternal spleens. Our results reveal that C1q was upregulated during early gestation, C1r, C1s, C2, C3 and C5b increased at day 25 of gestation and C4a and C9 peaked at days 13 and 16 of gestation. In addition, C3 protein was located in the capsule, trabeculae and splenic cords. In conclusion, our results show for the first time that there was modification in the expression of complement components in the ovine spleen at the early stage of gestation, and complement pathways may participate in modulating splenic immune responses at the early stage of gestation. View Full-Text
Keywords: pregnancy; complement pathway; immune regulation; spleen; sheep pregnancy; complement pathway; immune regulation; spleen; sheep
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MDPI and ACS Style

Yang, L.; Wang, L.; Wu, J.; Wang, H.; Yang, G.; Zhang, L. Changes in Expression of Complement Components in the Ovine Spleen during Early Pregnancy. Animals 2021, 11, 3183. https://doi.org/10.3390/ani11113183

AMA Style

Yang L, Wang L, Wu J, Wang H, Yang G, Zhang L. Changes in Expression of Complement Components in the Ovine Spleen during Early Pregnancy. Animals. 2021; 11(11):3183. https://doi.org/10.3390/ani11113183

Chicago/Turabian Style

Yang, Ling, Luyu Wang, Jiaxuan Wu, Haichao Wang, Gengxin Yang, and Leying Zhang. 2021. "Changes in Expression of Complement Components in the Ovine Spleen during Early Pregnancy" Animals 11, no. 11: 3183. https://doi.org/10.3390/ani11113183

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