Next Article in Journal
Seroprevalence and Clinical Outcomes of Neospora caninum, Toxoplasma gondii and Besnoitia besnoiti Infections in Water Buffaloes (Bubalus bubalis)
Previous Article in Journal
The Influence of Anaesthetic Drugs on the Laryngeal Motion in Dogs: A Systematic Review
Previous Article in Special Issue
Effect of Zinc Source and Exogenous Enzymes Supplementation on Zinc Status in Dogs Fed High Phytate Diets
Open AccessArticle

Interplay between Neuroendocrine Biomarkers and Gut Microbiota in Dogs Supplemented with Grape Proanthocyanidins: Results of Dietary Intervention Study

Department of Agriculture, Food, Environmental and Animal Science, University of Udine, 33100 Udine, Italy
*
Author to whom correspondence should be addressed.
Animals 2020, 10(3), 531; https://doi.org/10.3390/ani10030531
Received: 10 February 2020 / Revised: 20 March 2020 / Accepted: 20 March 2020 / Published: 22 March 2020
The connection between animal health and gut microbiota has been studied during the past years through different diet modulation experiments; however, there is still a paucity of information about the prebiotic functions in the gastrointestinal tract of companion animals. Considering this, a population of dogs living in the same environment has been subjected to a nutritional study, with different doses of proanthocyanidins extracted from grapevine supplied to the diet. Characterization of the gut microbiota and data from endocrine analysis in saliva have been collected. Dogs responded differently to the dietary intervention, and results underlined the existence of a difference between subjects in terms of fecal microorganisms and neuroendocrine markers, leading us to think the balance of gut microbiota is going to play a strong role in diet formulation based on host health modulation.
Several studies on the interaction between gut microbiota and diets, including prebiotics, have been reported in dogs, but no data are available about the effects of dietary administration of grape proanthocyanidins. In the study, 24 healthy adult dogs of different breeds were recruited and divided in 3 groups of 8 subjects each. A group was fed with a control diet (D0), whilst the others were supplemented with 1 (D1) or 3 (D3) mg/kg live weight of grape proanthocyanidins. Samples of feces were collected at the beginning and after 14 and 28 days for microbiota, short chain fatty acid, and lactic acid analysis. Serotonin and cortisol were measured in saliva, collected at the beginning of the study and after 28 days. A significantly higher abundance (p < 0.01) of Enterococcus and Adlercreutzia were observed in D0, whilst Escherichia and Eubacterium were higher in D1. Fusobacterium and Phascolarctobacterium were higher (p < 0.01) in D3. Salivary serotonin increased (p < 0.01) at T28 for D1 and D3 groups but cortisol did not vary. Proanthocyanidins administration influenced the fecal microbiota and neuroendocrine response of dogs, but a high variability of taxa was observed, suggesting a uniqueness and stability of fecal microbiota related to the individual. View Full-Text
Keywords: proanthocyanidins; fecal microbiota; end products of fermentation; serotonin; cortisol; Canis lupus familiaris proanthocyanidins; fecal microbiota; end products of fermentation; serotonin; cortisol; Canis lupus familiaris
Show Figures

Figure 1

MDPI and ACS Style

Scarsella, E.; Cintio, M.; Iacumin, L.; Ginaldi, F.; Stefanon, B. Interplay between Neuroendocrine Biomarkers and Gut Microbiota in Dogs Supplemented with Grape Proanthocyanidins: Results of Dietary Intervention Study. Animals 2020, 10, 531.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop