Calcium Electroporation of Equine Sarcoids
Center for Experimental Drug and Gene Electrotransfer (C*EDGE), Department of Clinical Oncology and Palliative Care, Zealand University Hospital, Sygehusvej 10, 4000 Roskilde, Denmark
Department of Clinical Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Blegdamsvej 3B, 2200 Copenhagen N, Denmark
Department of Pathology, Aarhus University Hospital, Palle Juul-Jensens Boulevard, 8200 Aarhus N, Denmark
Hørsholm Hestepraksis, Kongevejen 124, 3480 Fredensborg, Denmark
Author to whom correspondence should be addressed.
Received: 27 January 2020 / Revised: 16 March 2020 / Accepted: 16 March 2020 / Published: 19 March 2020
Sarcoids are skin tumors on horses where better treatment options are needed. Calcium electroporation is a novel anti-cancer treatment where calcium is injected into the tumor, followed by brief electric pulses (electroporation) that transiently open the cell membrane and allow uptake of calcium. This, in turn, induces cell death. In this study, we aimed to investigate the safety and effect of calcium electroporation on sarcoids. Thirty-two sarcoids in eight horses were treated once or twice with calcium electroporation and followed for 12–38 weeks with size-measurement and clinical photographs. The study showed that calcium electroporation is a safe and feasible treatment for sarcoids, including inoperable sarcoids. Six of 27 sarcoids (22%) were completely eliminated, and six further sarcoids (22%) decreased more than 30% in size. No relation between response and location, type, nor size of the sarcoid was observed. Larger studies are needed to further investigate the effect of calcium electroporation on sarcoids.