Identification and Quantification of Transient Receptor Potential Vanilloid 1 (TRPV1) in Equine Articular Tissue
by
Anne Frank Gallagher vom Braucke 1, Nanna Lysemose Frederiksen 1, Lise Charlotte Berg 1
, Stacie Aarsvold 2, Felix Christoph Müller 3, Mikael Ploug Boesen 4 and Casper Lindegaard 1,*
Anne Frank Gallagher vom Braucke 1, Nanna Lysemose Frederiksen 1, Lise Charlotte Berg 1, Stacie Aarsvold 2, Felix Christoph Müller 3, Mikael Ploug Boesen 4 and Casper Lindegaard 1,*
1
Large Animal Teaching Hospital, Department of Veterinary Clinical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, DK-2630 Taastrup, Denmark
2
Puchalski Equine Imaging, CA, Petaluma, CA 94954, USA
3
Department of Radiology, Herlev and Gentofte Hospital, DK-2730 Herlev, Denmark
4
Department of Radiology, Copenhagen University Hospital Bispebjerg and Frederiksberg, DK-2400 Copenhagen, Denmark
*
Author to whom correspondence should be addressed.
Animals 2020, 10(3), 506; https://doi.org/10.3390/ani10030506 (registering DOI)
Received: 10 February 2020 / Revised: 8 March 2020 / Accepted: 16 March 2020 / Published: 18 March 2020
(This article belongs to the Special Issue Large Animal Surgery—Novel Techniques and Current Best Practice to Improve Outcome)
Joint pain and osteoarthritis (OA) are some of the most common causes of lameness in horses, and most of the available treatments focus on symptomatic relief without a disease-modifying effect. TRPV1 is a potential target for treating joint diseases, including OA, and the present study aims to investigate if the TRPV1 receptor is present in equine articular tissue and determine whether the number of receptors is upregulated in joint inflammation. Metacarpo/metatarsophalangeal (MCP/MTP) joints from 15 horses euthanised for reasons unrelated to this study were included. Based on synovial fluid analysis, macroscopic evaluation, and magnetic resonance imaging (MRI), joints were divided into two groups: healthy joints and joints with pathology. ELISA analysis was performed on synovial tissue harvested from all joints. TPRV1 was found in all joints. The mean concentration of TRPV1 compared to total protein in healthy joints (8.4 × 10−7 ng/mL) and joints with pathology (12.9 × 10−7 ng/mL) differed significantly (p = 0.01, t-test with Welch correction). Quantitative real-time reverse transcriptase PCR analysis was performed on RNA isolates from synovial tissue from all joints. TRPV1 mRNA expression ratio normalized to glyceraldehyde 3-phosphate dehydrogenase (GAPDH) in healthy joints (0.16 (SD: 0.19)) and joints with pathology (0.24 (SD: 0.14)) did not differ significantly (p = 0.43, t-test with Welch correction). mRNA expression of interleukin-6 (IL-6) and tumor necrosis factor alpha (TNF-α) was very low for both groups. In conclusion, TRPV1 was detected both on mRNA and the protein level, with a higher expression of TRPV1 in samples from joints with pathology. Future studies will determine the clinical potential of equine TRPV1 as a target in the management of joint pain and inflammation.
View Full-Text
Keywords:
TRPV1; osteoarthritis; capsaicin; WORMS
▼
Show Figures
Figure 1
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
MDPI and ACS Style
vom Braucke, A.F.G.; Lysemose Frederiksen, N.; Berg, L.C.; Aarsvold, S.; Müller, F.C.; Ploug Boesen, M.; Lindegaard, C. Identification and Quantification of Transient Receptor Potential Vanilloid 1 (TRPV1) in Equine Articular Tissue. Animals 2020, 10, 506.
Show more citation formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.
