Next Article in Journal
The Role of Transcription Factors in Gonad Development and Sex Differentiation of a Teleost Model Fish—Guppy (Poecilia reticulata)
Previous Article in Journal
Linking Human Perceptions and Spotted Hyena Behavior in Urban Areas of Ethiopia
Article

Effect of the Refrigeration System on In Vitro Quality and In Vivo Fertility of Goat Buck Sperm

1
CITA—Instituto Valenciano de Investigaciones Agrarias, Polígono la Esperanza, 100, 12400 Segorbe, Castellón, Spain
2
ACRIMUR, C/Baron del Solar, 22-A, Edificio II, Entresuelo A, Puerta B, 30520 Jumilla, Murcia, Spain
3
Department of Animal Production and Health, Veterinary Public Health and Food Science and Technology (PASAPTA) Facultad de Veterinaria, Universidad Cardenal Herrera-CEU, CEU Universities, C/Tirant lo Blanc, 7, 46113 Alfara del Patriarca, Valencia, Spain
*
Author to whom correspondence should be addressed.
Animals 2020, 10(12), 2399; https://doi.org/10.3390/ani10122399
Received: 5 October 2020 / Revised: 26 November 2020 / Accepted: 9 December 2020 / Published: 15 December 2020
(This article belongs to the Section Animal Reproduction)
Artificial insemination of goats is mostly performed with sperm that has been chilled to 4 °C and stored for up to 12 h. Because the chilling of samples to 4 °C must be done slowly, artificial inseminations have to be performed late in the day, especially in herds located far from artificial insemination centers, which impedes the extensive use of this reproductive technology in goats. In this study, we explored the possibility of chilling sperm doses at a controlled rate (close to −0.18 °C/min) during transportation and compared the quality and fertility of these samples with those chilled in the lab. We observed that the in vitro quality of doses chilled by the prototype procedure developed for transportation was higher than that of samples chilled in the lab, and the fertility of the sperm was similar. Therefore, it is possible to chill doses during transportation without affecting their quality or fertility.
Cooling goat sperm insemination doses to 4 °C causes a delay in their delivery. However, chilling these doses during the transportation period could expedite their delivery and the insemination process. In this study, an economical and simple apparatus for chilling goat semen doses in itinere was developed, and the in vitro quality and in vivo fertility of these doses were compared with those chilled by means of a programmable water bath in the laboratory at a rate of −0.18 °C/min. Of the tested prototypes, the one that provided an optimal combination of the chilling rate (average of −0.09 °C/min) and time required to reach 4 °C (3 h 45 min) was selected for further testing. Immediately after chilling and 24 h later, the doses chilled in the prototype were determined to be of higher quality than the samples chilled in the programmable water bath. Finally, the kidding rate was similar between the doses chilled in the programmable water bath (61.7% ± 7.1%) and in the prototype (56.1% ± 5.9%). In conclusion, successful chilling of goat sperm doses during transport is possible, thereby accelerating the delivery of insemination doses. View Full-Text
Keywords: cooling; chilling; goat buck; semen; liquid-stored semen; refrigerated semen cooling; chilling; goat buck; semen; liquid-stored semen; refrigerated semen
Show Figures

Figure 1

MDPI and ACS Style

Mocé, E.; Lozano-Palazón, S.A.; del Mar Martínez-Granell, M.; Mocé, M.L.; Gómez, E.A. Effect of the Refrigeration System on In Vitro Quality and In Vivo Fertility of Goat Buck Sperm. Animals 2020, 10, 2399. https://doi.org/10.3390/ani10122399

AMA Style

Mocé E, Lozano-Palazón SA, del Mar Martínez-Granell M, Mocé ML, Gómez EA. Effect of the Refrigeration System on In Vitro Quality and In Vivo Fertility of Goat Buck Sperm. Animals. 2020; 10(12):2399. https://doi.org/10.3390/ani10122399

Chicago/Turabian Style

Mocé, Eva, Salvador A. Lozano-Palazón, María del Mar Martínez-Granell, María L. Mocé, and Ernesto A. Gómez. 2020. "Effect of the Refrigeration System on In Vitro Quality and In Vivo Fertility of Goat Buck Sperm" Animals 10, no. 12: 2399. https://doi.org/10.3390/ani10122399

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop