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Microorganisms
Volume 9
Issue 4
10.3390/microorganisms9040720
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Article

Detection of a New Resistance-Mediating Plasmid Chimera in a blaOXA-48-Positive Klebsiella pneumoniae Strain at a German University Hospital

by
Julian Schwanbeck
1,†,
Wolfgang Bohne
1,†,
Ufuk Hasdemir
2,
Uwe Groß
1,
Yvonne Pfeifer
3,
Boyke Bunk
4,
Thomas Riedel
4,5,
Cathrin Spröer
4,
Jörg Overmann
4,5,
Hagen Frickmann
6,7,‡ and
Andreas E. Zautner
1,*,‡
1
Institute for Medical Microbiology, University Medical Center Göttingen, 37075 Göttingen, Germany
2
Department of Medical Microbiology, School of Medicine, Marmara University, 34854 Istanbul, Turkey
3
Robert Koch Institute, FG13 Nosocomial Infections and Antibiotic Resistance, 38855 Wernigerode, Germany
4
Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, 38124 Braunschweig, Germany
5
German Center for Infection Research (DZIF), Partner Site Hannover-Braunschweig, 38124 Braunschweig, Germany
6
Department of Microbiology and Hospital Hygiene, Bundeswehr Hospital Hamburg, 20359 Hamburg, Germany
7
Institute for Medical Microbiology, Virology and Hygiene, University Medicine Rostock, 18057 Rostock, Germany
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
‡
These authors contributed equally to this work.
Microorganisms 2021, 9(4), 720; https://doi.org/10.3390/microorganisms9040720
Submission received: 17 March 2021 / Revised: 25 March 2021 / Accepted: 26 March 2021 / Published: 31 March 2021
(This article belongs to the Special Issue Molecular Epidemiology of Antimicrobial Resistance)
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Abstract

Mobile genetic elements, such as plasmids, facilitate the spread of antibiotic resistance genes in Enterobacterales. In line with this, we investigated the plasmid-resistome of seven blaOXA-48 gene-carrying Klebsiella pneumoniae isolates, which were isolated between 2013 and 2014 at the University Medical Center in Göttingen, Germany. All isolates were subjected to complete genome sequencing including the reconstruction of entire plasmid sequences. In addition, phenotypic resistance testing was conducted. The seven isolates comprised both disease-associated isolates and colonizers isolated from five patients. They fell into two clusters of three sequence type (ST)101 and two ST11 isolates, respectively; and ST15 and ST23 singletons. The seven isolates harbored various plasmids of the incompatibility (Inc) groups IncF, IncL/M, IncN, IncR, and a novel plasmid chimera. All blaOXA-48 genes were encoded on the IncL/M plasmids. Of note, distinct phenotypical resistance patterns associated with different sets of resistance genes encoded by IncL/M and IncR plasmids were observed among isolates of the ST101 cluster in spite of high phylogenetic relatedness of the bacterial chromosomes, suggesting nosocomial transmission. This highlights the importance of plasmid uptake and plasmid recombination events for the fast generation of resistance variability after clonal transmission. In conclusion, this study contributes a piece in the puzzle of molecular epidemiology of resistance gene-carrying plasmids in K. pneumoniae in Germany.
Keywords: Klebsiella pneumoniae; carbapenem resistance; beta-lactamase; resistome; plasmid; phylogeny; epidemiology Klebsiella pneumoniae; carbapenem resistance; beta-lactamase; resistome; plasmid; phylogeny; epidemiology

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MDPI and ACS Style

Schwanbeck, J.; Bohne, W.; Hasdemir, U.; Groß, U.; Pfeifer, Y.; Bunk, B.; Riedel, T.; Spröer, C.; Overmann, J.; Frickmann, H.; et al. Detection of a New Resistance-Mediating Plasmid Chimera in a blaOXA-48-Positive Klebsiella pneumoniae Strain at a German University Hospital. Microorganisms 2021, 9, 720. https://doi.org/10.3390/microorganisms9040720

AMA Style

Schwanbeck J, Bohne W, Hasdemir U, Groß U, Pfeifer Y, Bunk B, Riedel T, Spröer C, Overmann J, Frickmann H, et al. Detection of a New Resistance-Mediating Plasmid Chimera in a blaOXA-48-Positive Klebsiella pneumoniae Strain at a German University Hospital. Microorganisms. 2021; 9(4):720. https://doi.org/10.3390/microorganisms9040720

Chicago/Turabian Style

Schwanbeck, Julian, Wolfgang Bohne, Ufuk Hasdemir, Uwe Groß, Yvonne Pfeifer, Boyke Bunk, Thomas Riedel, Cathrin Spröer, Jörg Overmann, Hagen Frickmann, and et al. 2021. "Detection of a New Resistance-Mediating Plasmid Chimera in a blaOXA-48-Positive Klebsiella pneumoniae Strain at a German University Hospital" Microorganisms 9, no. 4: 720. https://doi.org/10.3390/microorganisms9040720

APA Style

Schwanbeck, J., Bohne, W., Hasdemir, U., Groß, U., Pfeifer, Y., Bunk, B., Riedel, T., Spröer, C., Overmann, J., Frickmann, H., & Zautner, A. E. (2021). Detection of a New Resistance-Mediating Plasmid Chimera in a blaOXA-48-Positive Klebsiella pneumoniae Strain at a German University Hospital. Microorganisms, 9(4), 720. https://doi.org/10.3390/microorganisms9040720

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