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Article

Epidemic HI2 Plasmids Mobilising the Carbapenemase Gene blaIMP-4 in Australian Clinical Samples Identified in Multiple Sublineages of Escherichia coli ST216 Colonising Silver Gulls

by
Hassan Tarabai
1,2,
Ethan R. Wyrsch
3,
Ibrahim Bitar
2,4,
Monika Dolejska
1,2,4,5,* and
Steven P. Djordjevic
3,*
1
Department of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Brno 612 42, Czech Republic
2
Central European Institute of Technology (CEITEC), University of Veterinary and Pharmaceutical Sciences Brno, Brno 612 42, Czech Republic
3
iThree Institute, University of Technology Sydney, Sydney, NSW 2007, Australia
4
Biomedical Center, Faculty of medicine in Pilsen, Charles University, Pilsen 323 00, Czech Republic
5
Department of Clinical Microbiology and Immunology, Institute of Laboratory Medicine, The University Hospital Brno, Brno 625 00, Czech Republic
*
Authors to whom correspondence should be addressed.
Microorganisms 2021, 9(3), 567; https://doi.org/10.3390/microorganisms9030567
Submission received: 31 January 2021 / Revised: 4 March 2021 / Accepted: 5 March 2021 / Published: 10 March 2021
(This article belongs to the Special Issue Molecular Epidemiology of Antimicrobial Resistance)
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Abstract

:
Escherichia coli ST216, including those that carry blaKPC-2, blaFOX-5, blaCTX-M-15 and mcr-1, have been linked to wild and urban-adapted birds and the colonisation of hospital environments causing recalcitrant, carbapenem-resistant human infections. Here we sequenced 22 multiple-drug resistant ST216 isolates from Australian silver gull chicks sampled from Five Islands, of which 21 carried nine or more antibiotic resistance genes including blaIMP-4 (n = 21), blaTEM-1b (n = 21), aac(3)-IId (n = 20), mph(A) (n = 20), catB3 (n = 20), sul1 (n = 20), aph(3”)-Ib (n = 18) and aph(6)-Id (n = 18) on FIB(K) (n = 20), HI2-ST1 (n = 11) and HI2-ST3 (n = 10) plasmids. We show that (i) all HI2 plasmids harbour blaIMP-4 in resistance regions containing In809 flanked by IS26 (HI2-ST1) or IS15DI (HI2-ST3) and diverse metal resistance genes; (ii) HI2-ST1 plasmids are highly related to plasmids reported in diverse Enterobacteriaceae sourced from humans, companion animals and wildlife; (iii) HI2 were a feature of the Australian gull isolates and were not observed in international ST216 isolates. Phylogenetic analyses identified close relationships between ST216 from Australian gull and clinical isolates from overseas. E. coli ST216 from Australian gulls harbour HI2 plasmids encoding resistance to clinically important antibiotics and metals. Our studies underscore the importance of adopting a one health approach to AMR and pathogen surveillance.

1. Introduction

Bacterial isolates resistant to antimicrobials have been isolated from wildlife [1] and there are concerns that wild animals act as critically important vectors and reservoirs for antimicrobial resistant bacteria and antibiotic resistance genes (ARGs) [2,3]. Migratory birds have the potential to spread multidrug resistant (MDR) bacteria and ARGs [4,5] posing a significant threat to biosecurity, particularly in countries that practice sound antimicrobial stewardship [1,6]. An increasing number of migratory birds have been found to host antimicrobial resistant bacteria with resistance to diverse antibiotics including those referred to as critically important (CIA) to human health such as the extended spectrum β-lactams, fluoroquinolones, carbapenems [7,8,9] and colistin [10,11,12,13].
Plasmids are vehicles that capture, assemble, maintain and spread ARGs genes, heavy metal resistance genes and virulence-associated genes (VAGs) [6] and provide flexibility to bacterial genomes by the diverse genetic cargo they carry [14]. Genes encoding resistance to antibiotics, heavy metals and virulence genes often coassemble on the same plasmid, mediated in part by the activity of insertion elements such as IS26 [15,16,17,18]. These factors allow the emergence of lineages that carry complex resistance regions and virulence gene profiles [14,19]. As such there is an urgent need to address the shortage of completed plasmid sequences in public databases and identify plasmids that carry virulence and antibiotic resistance genes [15,17,20] and plasmid hybrids [16,21]. Hybrid Escherichia coli carrying combinations of virulence genes from different pathovars are increasingly recognised as an emerging threat to human and animal health [22].
Dissemination of emergent and dominant multidrug resistant bacterial clades is a major driving force behind the global spread of antibiotic-resistant bacteria [14,23]. E. coli ST216 is known to carry genes encoding resistance to a broad range of antibiotics including those of clinical relevance [24,25,26]. However, little is known about ST216 virulence and antibiotic resistance gene cargo that it carries and the hosts it occupies. E. coli ST216 belongs to ‘commensal’ phylogroup A. With the exception of ST10 and STs belonging to clonal complex 10, phylogroup A has not been widely recognised as pathogenic in humans or non-human animals [27]. However E. coli belonging to the commensal phylogroups A and B1 are able to acquire virulence genes [17] and ARGs [28,29] and cause disease [17]. While ST216 is not one of the 20 most frequently reported E. coli STs on a global scale [27], MDR E. coli isolates of ST216 are recovered from humans with clinical infections [25,26,30,31]. Notably, Klebsiella pneumonia carabapenemase (KPC)-producing E. coli ST216 was linked with a large (125 isolates) and recalcitrant outbreak in Central Manchester University Hospital in the United Kingdom in 2015 [24]. During that episode, WGS showed that IncHI2 plasmids carrying blaKPC spread from ST216 to other E. coli STs as well as other Enterobacteriaceae. A notable feature of the UK outbreak was the recalcitrant nature of the contamination and the extraordinary measures taken to eliminate blaKPC+ ST216 from several cardiac wards by replacing plumbing infrastructure, a measure that only partially alleviated subsequent episodes of infection. The blaKPC-2 gene in the UK outbreak was a component of a Tn4401a transposon [24], known for its enhanced KPC expression [32] and this study, while notable, represents the only one retrieved by a PubMed search using “E. coli ST216” as a search parameter at the time of writing (26/12/2020). However, blaKPC-2 was shown to be associated with a Tn4401g transposon located on an N plasmid in a clinical isolate of E. coli ST216 in Israel [25] and hospital-acquired E. coli ST216 carrying blaFOX-5 (serine β-lactamase with a substrate specificity for cephalosporins) linked to an IncC plasmid were recovered from a senior patient at an intensive care unit in the University of Maryland Medical Center in the United States [26]. In a possible episode of patient to family transmission, two blaCTX-M-15-positive E. coli ST216 isolates were identified in a family member of a patient at Tel-Aviv Sourasky Medical Center in Israel [30] and colistin resistance was reported in an E. coli ST216 harbouring mcr-1 in a urine sample from a patient in Italy [31]. In the environment, E. coli ST216 carrying blaKPC-2 have been found on an R plasmid from a river ecosystem in Barcelona, Spain [33]. These episodes point to ST216 E. coli being proficient in the capture and dissemination of genes encoding β–lactamases and carbapenemases and suggest aquatic environments and both domestic and industrial wash basins and sinks may be an ideal niche. While speculative, the ability of ST216 to form recalcitrant biofilms in hospital wastewater pipes [24] is consistent with this view.
The silver gull typically breeds in large colonies on offshore islands and frequents urban environments including garbage dumps, shopping centres, railway stations, municipal parks and promenades along large river embankments. The Australian silver gull is known to harbour multiple drug-resistant E. coli [34,35] and Salmonella enterica spp. [36] highlighting hotspots where drug-resistant bacteria accumulate in urban environments [37,38]. In this study, we conducted whole genome sequencing of 22 MDR E. coli ST216 isolates from silver gulls (Chroicocephalus novaehollandiae) nesting on Big Island, 60 km south from Sydney in Australia, one of Australia’s largest silver gull breeding sites. We investigated their phylogeny and serotype composition and determined the antibiotic and virulence gene cargo they carry. Long read sequencing of plasmid DNA enabled the determination of the complete sequence of several plasmids that carry genes encoding resistance to CIA. Phylogenetic analysis of 123 MDR E. coli ST216 isolates and their plasmid content enabled an assessment of the spread of these isolates and their associated mobile elements and ARGs both within Australia and globally, and shed light on the potential risks they present to human and animal health.

2. Methods

2.1. E. coli ST216 Collection from Gulls

Bacterial isolates were obtained from our previous study investigating silver gulls (Chroicocephalus novaehollandiae) as carriers of antibiotic resistant bacteria in New South Wales, Australia [34]. In that study, cloacal samples (n = 504) from gull chicks were collected at three locations (Five Islands, White Bay in Sydney and Montague Island) and 27 IMP-producing E. coli ST216 were obtained, all originating from silver gulls at Five Islands. Based on PFGE genomic and plasmid profiles, six representative isolates were selected for further analysis. An additional 16 E. coli ST216 isolates were obtained by cultivation of primary cloacal samples enriched overnight in buffered peptone on MacConkey agar with cefotaxim (2 mg/L) or ciprofloxacin (0.05 mg/L). Their sequence types were determined following WGS of all E. coli isolates (n = 448) obtained from silver gulls at the three sampling locations (our unpublished data). A total of 22 E. coli ST216 isolates, all originating from gulls in Five Islands, were obtained and subjected to WGS (Supplementary Table S1).

Whole Genome Sequencing

Genomic DNA of 22 gull ST216 was isolated using NucleoSpin® Tissue kit (Macherey-Nagel GmbH & Co, Duren, Germany). DNA libraries were prepared using Nextera XT DNA library preparation kit and sequenced on a NovaSeq (Illumina, San Diego, CA, USA) platform. Assembly of obtained short reads was performed using Shovill v0.9.0 software [39].
Genomic DNA from E. coli CE1537 was selected for long read sequencing to obtain a complete E. coli ST216 reference sequence. Whole-genome DNA was extracted using NucleoSpin® Tissue kit (Macherey-Nagel GmbH & Co, Duren, Germany) and library preparation was performed using microbial multiplexing based on the manufacturer’s recommendation. The DNA was sheared using g-tubes (Covaris, Woburn, MA, USA) but size selection was not performed for library preparation. The Sequel 1 platform (Pacific Biosciences, Menlo Park, CA, USA) was used for long-read sequencing. Sequence assembly was carried with HGAP v4.0 software [40] and resulted in 11 contigs with an average 203-fold coverage. The incomplete sequence of an HI2-ST1 plasmid was identified on a separate contig, therefore, long-read sequencing using plasmid DNA of CE1537 was performed as described below.
Long-read sequencing of plasmid DNA (pDNA) extracted from isolates CE1537 and CE1681 was carried out to generate complete plasmid sequences. Plasmid DNA was extracted using a QIAGEN® midi kit (Qiagen, Hilden, Germany) and library preparation using a microbial multiplexing protocol was performed as described above. The Sequel 1 platform (Pacific Bioscinces, Menlo Park, CA, USA) was used for pDNA sequencing followed by assembly of obtained reads with SMRT LNK v8.0 software (Pacific Biosciences, Menlo Park, CA, USA). This resulted in the assembly of six circular contigs from isolate CE1537 (pCE1537-A to pCE1537-F) with an average 317-fold coverage and six contigs from isolate CE1681 (pCE1681-A to pCE1681-F) with an average 326-fold coverage (Supplementary Table S2).
FastQC (https://www.bioinformatics.babraham.ac.uk/projects/fastqc/) was used for quality control of obtained short reads and long read sequences (accessed on 15 February 2021-Table 1).

2.2. E. coli ST216 Metadata

All E. coli ST216 short reads were deposited on EnteroBase in the Escherichia/Shigella database and on Genbank (see Table 1 for barcode numbers and accession numbers, respectively) [41]. Short read sequences of ST216 isolate CE1537 were discarded due to contamination.
Long read sequence of isolate CE1537 was deposited on GenBank (accession number (AN): JABBCF000000001). Similarly complete and closed plasmids pCE1537-A (AN: MT232840), pCE1537-B (AN: MT162140), pCE1537-C (AN: MT162141), pCE1537-D (AN: MT162142), pCE1537-E (AN: MT162143), pCE1537-F (AN: MT162144), pCE1681-A (AN: MT180430), pCE1681-B (AN: MT180431), pCE1681-C (AN: MT180432), pCE1681-D (AN: MT180433), pCE1681-E (AN: MT180434) and pCE1681-F (AN: MT180435) were deposited in GenBank. Quality control on long read sequences of genomic DNA
For phylogenetic analysis genomes of E. coli ST216 (n = 99) from the EnteroBase Escherichia/Shigella database with one allele mismatch were selected and their assembly barcode used in the constructed phylogenetic tree (Figure 1). E. coli ST216 sequences with no sample collection date were excluded. In addition, one E. coli ST216 from Genbank with assembly and accession numbers GCA_002263825.1 and NNAL00000000 respectively, was used in ST216 phylogenetic analysis.

2.2.1. WGS Analysis

Publicly available tools were used to analyse short and long read sequences of E. coli ST216 gull and Enterobase sequences. The affiliation to sequence type, serotype and fimH type were confirmed using MLST (v2.0), SerotypeFinder (v.2.0) and FimTyper (v.1.0), respectively (accessed on 13 August 2020 and available at https://cge.cbs.dtu.dk/services/). E. coli ST216 phylogroup was assigned using Clermont Typing (accessed on 13 August 2020 and available at http://clermontyping.iame-research.center/). ResFinder v.3.2 (accessed on 15 August 2020 and available at https://cge.cbs.dtu.dk/services/) and CARD [42] and Virulence Factor DataBase (VFDB) [43] were used to identify antibiotic resistance and virulence genes, respectively. Plasmid replicons and plasmid ST were determined using PlasmidFinder (v.2.0) and pMLST (v.2.0) respectively (accessed on 15 August 2020 and available at https://cge.cbs.dtu.dk/services/). Automated annotation of all sequences was generated by RASTtk [44].
Complete sequences of pDNA from isolates CE1537 and CE1681 (Supplementary Table S2) were assessed for plasmid types, ARGs, VAGs and phages. The metal resistance genes content of closed plasmids was evaluated using BacMet (accessed on 25 August 2020 and available at http://bacmet.biomedicine.gu.se/blast/blast_link.cgi). Automated annotation of complete plasmid sequences was generated by RAST-tk [44] followed by manual curation using SnapGene® v5.0.6 (GSL Biotech LLC, Chicago, IL, USA) and a BLASTn online tool (NCBI, Rockville, MD, USA). Insertion sequences and phage-associated genes within closed contigs (Supplementary Table S2) were searched by ISFinder [45] and PHASTER [46].

2.2.2. Antibiotic Susceptibility Testing of Gull Isolates

Susceptibility to 15 different antibiotics of gull-sourced E. coli ST216 isolates was tested using the disk diffusion method according to European Committee on Antimicrobial Susceptibility Testing (EUCAST) recommendations [47] using the following antibiotic discs (Oxoid, Hants, UK): amoxicillin-clavulanic acid (20–10 µg), ampicillin (10 µg), cefalotin (30 µg), ceftazidime (30 µg), chloramphenicol (30 µg), ciprofloxacin (5 µg), ertapenem (10 µg), gentamicin (10 µg), imipenem (10 µg), nalidixic acid (30 µg), meropenem (10 µg), sulphonamide compounds (300 µg), streptomycin (10 µg), tetracycline (30 µg) and trimethoprim-sulfamethoxazole (1.25/23.75 µg). Measuring and interpretation of inhibition zone diameters of the tested isolates was performed according to EUCAST breakpoints [48] or using breakpoints defined by CLSI 2017 [49] for antibiotics (azithromycin, cefazolin, tetracycline, nalidixic acid, sulphonamide compounds and streptomycin) with no defined breakpoints in EUCAST 2019 [48]. E. coli ST216 isolates were tested for susceptibility to colistin using colispot test [50]. AmpC, extended-spectrum beta-lactamase and carbapenemase production in E. coli ST216 isolates was assessed using AmpC, ESBL and Carbapenemase Set D72C (Mast Diagnostics, Merseyside, UK) and carbapenemase production was confirmed with matrix-assisted laser desorption ionisation-time of flight mass spectrometry [51]. ST216 that are nonsusceptible to at least one antibiotic in three or more antibiotic classes were deemed to be multidrug-resistant [52].

2.2.3. Phylogenetic Analysis

Single nucleotide polymorphisms (SNPs) (Figure 1 and Supplementary Figure S1) were identified and used for phylogenetic analysis of ST216 using CSI Phylogeny 1.4 [53]. A second SNP analysis (Supplementary Figure S2) including only gull E. coli ST216 isolates sequenced in this study was also performed. Phylogenetic trees were visualised with iTOL v4 [54]. Comparison and alignment of long- and short-read sequences with E. coli reference genomes from K12-MG1655 and E. coli ATCC8739 (GenBank Accession no.: U00096.3 and NZ_CP022959.1, respectively) were performed using progressiveMauve [55].

2.2.4. Phylogenetic Analysis of Complete and Closed Plasmids

To investigate the distribution of closed contigs (pCE1537-A, pCE1537-B, pCE1681-A, pCE1681-B, pCE1681-D) within the genome sequences of E. coli ST216 sourced from gulls, heat maps were generated and visualised using the chooklord pipeline (accessed on 4 March 2021 and available at https://github.com/maxlcummins/chooklord [56]) with closed plasmids as reference (Figure 5A,B and Supplementary Figure S3A,E). A BLASTn search was performed and complete plasmid sequences with an identity threshold of ≥99% and a query coverage threshold of ≥91% were selected for further analysis (Table 2). A different coverage threshold (86%) was set for investigating plasmid pCE1681-E because pEc1677, which showed the highest detected coverage (86%) with pCE1681-E, was selected from a BLASTn analysis. BRIG v0.95 software [57] and SnapGene® v5.0.6 software (GSL Biotech LLC, Chicago, IL, USA) were used to perform comparisons of plasmid sequences. IS26-associated regions of plasmids pCE1537-A were subjected to further analysis using Easyfig v2.2.3 [58] for comparison and visualisation.

2.2.5. Transferability of HI2 Plasmids

The MDR profile of sequenced E. coli ST216 isolates (CE1537 and CE1681) prevented the performance of a direct conjugation assay with a suitable recipient (employing a unique selection marker from the donor cells). We performed a multiphase test to circumvent this obstacle and check the conjugative properties of the resolved HI2 plasmids. HI2 plasmids were selected for transferability testing due to their carriage of blaIMP-4 which was a primary focus for this study. Plasmid DNA was extracted from donor cells using the QIAGEN® midi kit (Qiagen, Hilden, Germany) and then transferred via electroporation to plasmid-free E. coli Top10 cells. Transformants positive for HI2 plasmid were selected on LB agar supplemented with cefotaxime (2 mg/L) and incubated overnight at 37 °C. The presence of blaIMP and the HI2 plasmid in transformants was confirmed by PCR [34] and replicon typing [59], respectively. Total cellular DNA from transformants was digested with S1 nuclease and subjected to pulsed-field gel electrophoresis to confirm plasmid carriage. Conjugative transfer of HI2 positive transformants to a plasmid-free, rifampicin and sodium-azide resistant E. coli MT102 recipient strain [60] was performed using filter-mating method with incubation for 4 h at 28, 30 and 37 °C followed by selection of transconjugants on LB agar plates supplied with cefotaxime (2 mg/L), rifampicin (25 mg/L) and sodium azide (100 mg/L) and incubation overnight at 37 °C. The presence of blaIMP and the HI2 plasmid in four transconjugants was confirmed by PCR [34] and replicon typing [59], respectively.

3. Results

3.1. Population Structure of Gull E. coli ST216

E. coli ST216 represents 8.4% of all E. coli isolates (n = 262) obtained from cloacal samples at Big Island and 5% of the 448 E. coli recovered from the three sampling sites (Five Islands, Sydney and Montague Island) (Supplementary Table S1). All 22 E. coli ST216 isolates that were sequenced are phylogroup A. In silico O:H typing identified two serotypes: O45:H4 (n = 1) and O154:H4 (36%, n = 8). Thirteen isolates were O-non-typable with flagella type H4 (9%, n = 2), O-non-typable with H-non-typable (36%, n = 8) and O45 with H-non-typable (14%, n = 3). fimH profiling revealed three fimH types including fimH23 (36%, n = 8), fimH69 (45.5%, n = 10), and fimH1248 (18%, n = 4) (Supplementary Table S1).

3.2. Phylogenetic Analysis of E. coli ST216

A SNP-based phylogenetic tree was constructed with genomes of 122 ST216 including the 22 E. coli ST216 isolates from silver gulls and 20 isolates with STs that carried a variant in a single multilocus sequence allele from ST216 (Figure 1). All isolates, including those from silver gulls, segregated into two main clades with significant diversity between them (3000–9000 SNP variants). Silver gull isolates also divided into two main SNP cluster groups (SCG) and six phylogenetic subgroups interspersed throughout the SNP-tree (Figure 1). Isolates from silver gulls (n = 22) were phylogenetically diverse showing a minimum of six and maximum of 8505 SNPs differences. The first SNP cluster (SCG1) which included eight E. coli ST216 isolates that differed only by 11–52 SNPs was located on a distinct branch (clade I in Figure 1) that included a handful of clinical isolates from the USA and a single clinical isolate from Norway. The gull cluster in clade I was most closely aligned (maximum of 283 SNPs difference between SCG1 and ESC_QA4689AA_AS) to the clinical isolate from Norway (ESC_QA4689AA_AS) (Figure 1). ST216 gull isolates in the second cluster (SCG2, n = 14) were more distinct (6–2347 SNPs difference) and included isolates from the clinic, environment, and domestic animals (clade II in Figure 1). Gull isolate 1720H in clade II was related (163–259 SNPs difference) to domestic animal isolates (ESC_TA2295AA_AS and ESC_GA6917_AS) from Australia and the USA, clinical isolates (ESC_ZA4597AA-AS and ESC_AA8402AA_AS) from Kenya and the USA and to an environmental isolate (ESC_GB5355AA_AS) of unknown origin.
E. coli ST216 isolates from silver gulls had a higher content of plasmids and ARGs (mean of 4.5 plasmids and 15 ARGs in silver gulls) compared to international ST216 isolates (mean of 1.7 plasmids and 1 ARG; Supplementary Figure S1), an observation that is consistent with selection on media containing antibiotics. In contrast to SCG1 and SCG2, HI2 plasmids were absent from almost all of the 100 international isolates. FIB(K) plasmids were the most common plasmids in international isolates (31%, n = 31) and were dominant in SCG1 and SCG2 (91%, n = 20). R plasmids were present in international isolates (11%, n = 11) and SCG2 (50%, n = 7) but absent from SCG1. Similarly, X5 plasmids were dominant in SCG2 (71%, n = 10) and not detected in SCG1. Resistance genes blaIMP-4, blaSHV-12 and dfrA19 were only identified in SCG1 and SCG2 and were not detected in any international isolate (Supplementary Figure S1) indicating that HI2 plasmids harbour genes encoding these CIA. Other ARGs including qnrS1, aac(6’)-Ib-cr and dfrA14 were identified both in SCG2 (71%, 93%, 64%, n = 10, n = 13, n = 9, respectively) and in international isolates (6%, 10% and 4%; n = 6, n = 10, n = 4, respectively) (Supplementary Figure S1). VAGs in SCG1, SCG2 and international isolates were common to E. coli species (Supplementary Figure S1). However, several VAGS were only present in SCG2 and international isolates including putative type III secreted effector espX1, fimbrial associated genes fimC, fimD, fimE and fimI and general secretion pathway genes gspC, gspD, gspE, gspF, gspI and gspK (Supplementary Figure S1).

3.3. Virulence Associated Genes (VAGs) of Gull Isolates

Carriage of virulence genes by the 22 ST216 isolates was unremarkable. Between 18 and 33 VAGs previously described among E. coli were identified in ST216 sequences. VAGs included enterobactins and elements of type II/III secretory systems, type I fimbriae regulators, ferric enterobactin transport system and general secretion pathway proteins (Supplementary Table S1). In isolate CE1537, a flagellin gene fliC was located on an FIA(HI1) plasmid (pCE1537-B) with a fliC repressor gene residing between two IS elements (Supplementary Figure S4-B and Supplementary Table S2). In isolate CE1681, a colicin E7 operon that includes the colicin E7 protein, colicin E7 immunity protein and a colicin E7 lysis protein is located on a Col156 plasmid (pCE1681-C) (Figure 2). In the same isolate (CE1681), a haemolysis expression-modulating protein (Hha) is located on X5 plasmid pCE1681-E (Figure S4-C).

3.4. Antibiotic Resistance Phenotypes and Genes of Gull Isolates

The collection of 22 sequenced isolates present variable MDR phenotypes with resistance ranging from 4 to 15 antibiotics (Supplementary Table S1). Most isolates (95%, 21/22) are carbapenemase producers (Supplementary Table S1). All isolates are resistant to streptomycin and sulphonamides (100%, 22/22) with 95% (21/22) of the isolates resistant to ampicillin, trimethoprim/sulfamethoxazole, cefalotin, ceftazidime and amoxicillin/clavulanic acid. Resistance to chloramphenicol and gentamicin were both observed in 91% (20/22) of the isolates and most ST216 isolates (19/22, 86%) are also resistant to tetracycline. Additional resistance to nalidixic acid, ertapenem, imipenem and meropenem was detected in 68% of isolates (15/22) while resistance to ciprofloxacin was present in 45% (10/22) of isolates. In summary, almost all ST216 are classified as multidrug resistant and express resistance to clinically important antibiotics.
We identified a total of 30 ARGs in the sequenced population of gull E. coli ST216 isolates and the strains carry between 7 and 20 ARGs each (Supplementary Table S1). A total of 21 out of 22 (95.45%) isolates carried nine or more ARGs. The most common ARGs among the ST216 isolates included blaIMP-4 (95.5%, n = 21), blaTEM-1b (95.5%, n = 21), aac(3)-IId (91%, n = 20), mph(A) (91%, n = 20), catB3 (91%, n = 20), sul1 (91%, n = 20), aph(3”)-Ib (82%, n = 18) and aph(6)-Id (82%, n = 18) (Supplementary Table S1). The quinolone resistance genes qnrA1 (36%, n = 8), qnrS1 (45.5%, n = 10) and aac(6’)-Ib-cr (45.5%, n = 10) were less frequent. All ST216 isolates carried a class 1 integrase intI1 and IS26 (Supplementary Table S1). The resistance phenotype of most ST216 isolates correlated with their genotype except for isolate CE1681 and 1720H which exhibited phenotypic resistance to trimethoprim and chloramphenicol, respectively, with the absence of corresponding ARGs (Supplementary Table S1).
Most E. coli ST216 (95.5%) have at least one plasmid-mediated quinolone resistance gene (21/22) with only nine isolates expressing phenotypic resistance to ciprofloxacin and nalidixic acid. Of the 22 ST216 isolates, six are resistant only to nalidixic acid, one isolate is resistant only to ciprofloxacin while six isolates are susceptible to ciprofloxacin and nalidixic acid (Supplementary Table S1). Resistance to ertapenem, meropenem and imipenem was detected in 15/21 blaIMP-4-positive E. coli ST216 isolates (Supplementary Table S1). Known chromosomal gyrA mutation S83L that confers resistance to nalidixic acid and ciprofloxacin was identified in isolates 1556m1, 1548R1 and 1605m2 (Supplementary Table S1) [61]. Moreover, several chromosomal mutations with unknown effect were detected in 16S_rrsB, 16S_rrsC, 16S_rrsH, 23S, pmrB, parC and gyrA genes for isolates CE1537 and CE1586. Isolate CE1586 had an additional chromosomal mutation in pmrA gene.
In all blaIMP-4-positive E. coli ST216 isolates, blaIMP-4 was found on HI2-ST1 plasmids as a component of In809 that was flanked by IS26 (Figure 3) (n = 11, Figure 4 and Figure 5A) or on HI2-ST3 plasmids flanked by IS26 variant, IS15DI [62] (n = 10, Supplementary Figure S4-A and Figure 5B).

3.5. Plasmids Identified in ST216 Isolates

Among the 22 ST216 WGS, plasmid families HI2 (95%, n = 21) and FIB(K) (91%, n = 20) with F-:A13*:B-(n = 5), F-:A18*-:B- (n = 3), F100:A-:B- (n = 2) F2:A13*:B- (n = 1), F2:A18*-:B- (n = 1) and F100:A13*-:B- (n = 1) were observed. Other plasmid replicons included FIA(HI1) (50%, n = 11), IncY (50%, n = 11), X5 (45%, n = 10), Col156 (n = 9, 41%), R (32%, n = 7), Col4401, FII and Q1 (18%, n = 4), X1 (14%, n = 3) and Col(BS512) (4.5%, n = 1) (Supplementary Table S1). Matches to Q1 plasmids need to be interpreted with caution as transposons such as Tn6029 and Tn6026 contain a Q1 rep and these transposons are encountered frequently [63]. Plasmid profiles ranged from one to eight plasmids among the 22 ST216 genomes. HI2 family plasmids were of two sequence types including HI2-ST1 (50%, n = 11) and HI2-ST3 (45%, n = 10) and all carried blaIMP-4 (Supplementary Table S1). Interestingly, the HI2-ST3 plasmid was observed with a single acquired N plasmid rep gene, giving an apparent hybrid plasmid type.
Phylogenetic analysis of closed plasmids revealed a higher diversity in the MDR region of HI2-ST1 plasmids compared to HI2-ST3 plasmids (Figure 5A,B, respectively). With the exception of 1560H showing variability in its plasmid backbone sequence and 1660m1 with a variable MDR region, HI2-ST3 plasmids were highly homologous (Figure 5B). Similarly, R plasmids were indistinguishable among the gull isolates except for isolate 1605m2 which showed sequence deviation in its plasmid backbone (Supplementary Figure S3D). Isolate 1605m2 had variable plasmid, ARGs and VAGs profiles and shared common fimH 69 and O154:H4 types with nine and six ST216 isolates, respectively. However, sul3, cmlA1 and dfrA12 were the only ARGs found in 1605m2 (Supplementary Table S1). FIB(K) and FIA plasmids were not typeable while three FII plasmids belong to ST2 (n = 2) and ST100 (n = 1) (Supplementary Figure S1). The MDR region in FIB(K) plasmids, observed in six isolates, showed high sequence identity (Supplementary Figure S3B). Plasmids Col156, which carry the colicin E7 operon (Figure 2) and X5 that carry flagellar transcriptional modulator gene (flhD) and haemolysin expression modulator (hha) (Supplementary Figure S4-C) were distributed among E. coli ST216 isolates with a prevalence of 41% (n = 9) and 45% (n = 10), respectively (Supplementary Figure S3C,E).

3.6. Analysis of HI-ST1, HI2-ST3, FIA, FIB(K), X5, R and Col156 Plasmids

Twelve complete and closed plasmids including HI2-ST1, HI2-ST3, FIA, FIB(K), X5, R, Y, Col156, ColE and Col440I were generated by long read sequencing of isolates CE1681 (n = 6) and CE1537 (n = 6) (Supplementary Table S2). From those 12 plasmids, only five (pCE153-A, pCE1681-A, pCE1681-B and pCE1681-D) carried antibiotic resistance genes while two (pCE1681-C and pCE1537-C) carried the colicin E7 operon (Supplementary Table S2).
HI2-ST1 plasmid pCE1537-A had a backbone structure typical of HI2 plasmids, an Inc type broadly identified previously in 350 commensal E. coli from Australian swine (Figure 4) and was shown to be conjugative after its conjugation into a recipient E. coli [64]. It carried a complex resistance locus that included genes for resistance to aminoglycosides (aac(3)-IId, aph(3’’)-Ib, aac(6’)-Ib3, acc(6’)-Ib-cr and aph(6)-Id), beta-lactams (blaIMP-4, blaOXA-1, blaTEM-1b and blaSHV-12), fluoroquinolones (aac(6’)-Ib-cr), rifampicin (arr-3), phenicol (catA2 and catB3), sulphanomides (sul1), tetracycline (tet(D)) and macrolides (mph(A)) (Supplementary Table S2). A large portion of the resistance region forms a pseudo-compound transposon flanked by copies of IS26 in the same orientation and included other IS elements (Figure 3). The carbapenemase gene blaIMP-4 was observed as a gene cassette in an In809 class 1 integron (Figure 3). Analysis of metal resistance operons identified genes associated with resistance to arsenic, mercury, tellurium, lead, copper, cobalt, zinc, cadmium as well as several multidrug efflux transporters (Figure 4). Moreover, plasmid pCE1537-A contained resistance genes for formaldehyde (frmB and frmR) and ethidium bromide (emrE) (Figure 4).
HI2-ST3 plasmid pCE1681-A (Supplementary Table S2 and Supplementary Figure S4-A) carried genes that confer resistance to aminoglycosides (aac(6’)-Ib-cr, aac(3)-IId), beta-lactams (blaIMP-4, blaOXA-1 and blaTEM-1b), fluoroquinolones (aac(6’)-Ib-cr), rifampicin (arr-3), macrolides (mph(A)), phenicol (catB3), sulphanomide (sul1) and tetracycline (tet(A)). Two class one integron structures were identified in pCE1681-A (Supplementary Figure S4-A). The first contained mph(A), sul1, catB3, blaOXA-1 and aac(6’)-Ib-cr and several IS elements. The second, much smaller structure harboured blaIMP-4 hosted in the integron, flanked by inward-oriented IS6 elements, with noted mutations for variant IS15DI [62]. MRGs for mercury and tellurium were also detected on pCE1681-A (Supplementary Figure S4-A).
FIA plasmid pCE1537-B found in isolate CE1537 did not harbour ARGs but carried a flagellin (fliC) encoding an Hx allele and a fliC repressor gene. These genes were flanked by an IS630-like element and ISSen4 (Supplementary Table S2 and Supplementary Figure S4-B). Y plasmid pCE1537-E accommodated an intact phage (Escher_RCS47_NC_042128) which was flanked by an IS26-like element upstream and an IS26 element downstream of its sequence.
FIB(K) plasmid pCE1681-B, found in isolate CE1681 harboured genes for resistance to aminoglycoside (aph(6)-Id and aph(3′′)-Ib), beta-lactam (blaLAB-2 and blaTEM-1b), fluoroquinolones (qnrS1), phenicol (catA2), sulphanomide (sul2) and trimethoprim (dfrA14). All the ARGs were part of an atypical class 1 integron structure that included four IS26 elements (Figure 6).
Only a single ARG (blaTEM-1b) and several arsenic MRGs were identified on IncR plasmid pCE1681-D (Supplementary Table S2 and Figure S4-D). X5 plasmid pCE1681-E harboured several formaldehyde detoxification genes as well as flhD and hha (Supplementary Figure S4-C). Col156 plasmid pCE1681-C contained a DNase-bacteriocin operon encoding colicin E7 (Figure 2) [65].
Based on preset criteria (Table 2) four plasmids were selected for comparison with pCE1537-A (HI2-ST1). HI2 plasmids, pAUSMDU8141-1, pC15_001, pIMP4-SEM1 and pMS7884A showed high sequence identity in their backbone sequences but variable MDR regions. They all originated from Australia and two of them (pIMP4-SEM1 and pMS7884A) carried blaIMP-4 (Figure 3 and Figure 4 and Table 2). However, these four plasmids (Table 2) were identified from different bacterial species from different geographic locations with pAUSMDU8141-1, pC15_001 and pMS7884A originating from clinical sources in Victoria, Sydney and Brisbane, respectively. pMS7884A is from Enterobacter hormaechei strain MS7884A that carried blaIMP-4 while pC15_001, also from E. hormaechei, harboured blaOXA-1. Both plasmids were associated with clinical outbreaks in the Intensive Care Unit (ICU) of Concord Repatriation Hospital in Sydney between 2006 and 2015 [66] and an ICU and burns facility at a Brisbane hospital in 2015 [67], respectively. Plasmid pIMP4-SEM1 originated from a Salmonella enterica isolate colonising a feline companion animal [68].
A comparison of the blaIMP-4-containing In809 integron and IS26-flanked regions within MDR genes of pCE1537-A, pMS7884A (clinical source), pIMP4-SEM1 (companion animal source) and pC15_001 (clinical source) revealed high sequence identity (Figure 3). Translocations of qacG and qacE were evident between pCE1537-A and pMS7884A plasmids and an ISVsa3 transposase (IS91 family) replaced blaIMP-4 on pC15_001 (Figure 3). We did not perform comparative analyses with pCE1681-A (HI2-ST3 plasmid).
pCE1681-B shared high sequence coverage and identity with five plasmids (Table 2). Four of these pCFSAN061762, pFZ11, pCFSAN061763 and pCFSAN061768 were from E. coli and CP020344.1 was from Shigella flexneri. These five plasmids carried qnrS1, blaTEM-1b and dfrA14 (Figure 6). Plasmids CP020344.1 and pFZ11 had a clinical source in China while plasmids pCFSAN061762, pCFSAN061763 and pCFSAN061768 originated from raw milk in Egypt (Table 2).
Analysis of plasmid pCE1681-C showed 100% coverage and ≥ 99% identity with two E. coli plasmids (Table 2), all carrying a colicin E7 operon (Figure 2). Plasmids ColE7-K317 and pECAZ146_5 originated from an unidentified source in Pakistan and from a clinical source in Italy, respectively.
Plasmid pCE1681-E showed 86% coverage and 100% identity to a blaIMP-4-positive X5 plasmid pEc1677 (Supplementary Figure S4-C) originating from an E. coli isolate from a silver gull in Sydney [69]. The two plasmids had a similar backbone structure but differed in their variable region with the absence of ARGs in pCE1681-E while pEc1677 harboured blaIMP-4 and other ARGs. In plasmid pEc1677 blaIMP-4 was part of a class 1 integron that included antibiotic resistance genes aac(6′)-Ib3, catB3 and sul1 and quaternary ammonium resistance genes qacG and qacE. The organisation of these genes was like that observed in pCE1537-A (HI2-ST1) (Figure 4). Both plasmids, pEc1677 and pCE537-A shared an IS26 element upstream of blaIMP-4 while they had IS elements IS4321 and IS91-like bordering sul1.

4. Discussion

Wild and urban-adapted birds carry, cycle, and transmit mobile elements carrying ARGs and VAGS between humans, animals and the environment [3,35,36,70,71]. Carriage of MDR bacteria by wild and urban bird populations remind us of the need to remove anthropogenic pollutants, particularly antibiotic resistant bacteria, antibiotic residues, disinfectants and metals from the environment.
E. coli with variable MDR profiles including carbapenem resistance encoded by blaIMP-4 carried on HI2-ST1 and HI2-ST3 plasmids have been recovered from cloacal samples of silver gulls in Australia [34]. Based on this report, we utilised WGS to detect and investigate E. coli that carry genes encoding resistance to CIA. After ST457 [35], E. coli ST216 represented the second most common E. coli ST accounting for 8.4% of all E. coli isolates (n = 262) obtained from cloacal samples at Big Island and 5% of the 448 E. coli recovered from the three coastal sampling sites (Five Islands, Sydney and Montague Island) in New South Wales, Australia. SNP-based phylogenetic analysis of international ST216 isolates (Figure 1) divided ST216 into two clades. Clade I is small and includes isolates of clinical and domestic animal origin in addition to isolates sequenced here from silver gulls (SCG1). ST216 genomes in SCG1 show a clonal-like distribution and many strains carry HI2-ST1 plasmids that are highly similar to HI2-ST1 plasmids observed in Australian clinical and companion animal sources (Figure 4). Documented variability in the carriage of genetic cargo residing in complex resistance regions of HI2-ST1 plasmids in SCG1 (Figure 5A) suggest that multiple plasmid acquisition events may occur or that the resistance regions respond rapidly to selection pressures where ST216 persists.
The phylogeny of ST216 genomes in SCG2 (Figure 1) indicates that E. coli ST216 is globally distributed and occupies a diverse host range, including isolates from environmental sources as well as from humans, domestic animals and wildlife (Supplementary Figure S1). The distribution of SCG2 into several subphylogenetic groups (Figure 1) coupled with the diverse plasmid content compared with SCG1 (Figure 1 and Supplementary Table 1) suggest multiple introduction events of ST216 in silver gulls.
The risk of dissemination of E. coli ST216 carrying blaIMP-4 and genes encoding resistance to other CIA to humans and domestic animals is a cause for concern. Silver gulls in the Sydney-Wollongong region share common spaces that include facilities with high human contact (rail and bus stations and municipal parks) and ST216 has been isolated from silver gulls in different geographic regions of Australia [72]. Plasmids carrying multidrug resistance genes with different incompatability markers have been recovered from multiple E. coli and Salmonella enterica lineages from gulls in Australia [34,35,36,72]. E. coli ST216 show a clear propensity to acquire plasmids that carry diverse resistance gene cargo and are linked to aquatic environments where they have been responsible for recalcitrant, carbapenem-resistant infections in hospital drainage waste systems [24]. ST216 belongs to commensal phylogroup A. Apart from the outbreak cluster in the UK, most reports of ST216 in humans are sporadic cases [25,30] in hospital. There is a clear association of E. coli ST216 with wildlife and reports to date all describe carriage of genes encoding resistance to CIA [33,72] [This study]. Given its comparatively low reporting in humans and its repeated links with wildlife and aquatic environments it is tempting to speculate that water may be a natural reservoir for E. coli ST216 and aquatic wildlife hosts, particularly birds, for its distribution. The pressing question is what role wildlife will play, if any, in the continued evolution of E. coli ST216 given the propensity for it to acquire self-replicating mobile genetic elements.
The ARG content in our ST216 population (Supplementary Table S1) is largely due to the carriage of HI2 (ST1 and ST3) and FIB(K) plasmids (Supplementary Table S2). Isolation of Enterobacterales from gulls using antibiotic selection likely created a bias in what lineages were observed and explains the high carriage of plasmids and ARGs we found in the silver gulls. Comparisons to strains not isolated under an antibiotic selective pressure will be necessary to reveal the distribution of the now identified AMR genes and their transfer mechanisms. Insertion sequences IS26 and IS15DI play a major role in capturing and mobilising antibiotic resistance genes [62,73,74] and are often found in close association with class 1 integrons [64,75,76,77]. The presence of these insertion elements serves as a hotspot for capture of ARGs flanked by IS26 [73]. IS26 also plays an important role in altering the structure of class 1 integrons by truncating the 3’-CS and the 3´ end of intI1 [77,78,79]. IS26 has played a role in shaping the resistance regions in HI2 ST1 and ST3 and F plasmids that are carried by E. coli ST216 from silver gulls described in this study. IS26 elements can facilitate hybrid plasmid formation [16,80] and may have a role in promoting plasmid stability [81]. Comparative analysis of HI2-ST1 plasmid pCE1537-A provides evidence that plasmids circulate closely within different bacterial species and hosts (humans, companion animals and wildlife) in Australia. It is also concerning that IS26 has been implicated in the mobilisation of virulence genes [82].
F plasmids carrying ARGs and VAGs are widespread and are commonly associated with Enterobacterales from clinical sources and food-animals [83,84]. Isolates from silver gulls carry various VAGs, metal transport systems and the colicin E7 operon on several plasmids. The acquisition of these genetic elements can enhance the survival, colonisation and persistence characteristics of bacteria that inhabit different niches [65,85,86,87,88]. Moreover, the presence of both metal resistance genes and ARGs leads to coselection and promotion of ARGs in bacterial populations in the absence of antibiotics [89]. These data suggest that the evolution of ST216 and their mobilome are influenced by anthropogenic pollution. Feeding and flight behaviours [90] are likely to have a profound influence on the silver gull resistome.
At the time of writing, a total of 137 ST216 isolates were deposited in the Enterobase database. Of these 121 (80%) were deposited after 2010 with a global distribution across clinical, environmental, and animal hosts. Only a few of the ST216 isolates were collected from clinical samples associated with diarrhoea (ESC_FB7867AA and ESC_FB7867AA) in China and septicaemia (ESC_AA2218AA) in Germany. The increased frequency of reports of E. coli ST216 is concerning, particularly in light of their ability to acquire multiple diverse plasmids carrying ARGs, VAGS, biocins and metal resistance genes and their ability to colonise the gastrointestinal tracts of wild and urban birds. These characteristics are known to be important in the evolution of successful MDR bacterial clades [91]. However, unlike many dominant bacterial clades that show high genetic conservation within a geographical region [14], ST216 isolates appear to be phylogenetically interspersed and distanced from each other even within a single geographical location, suggesting that flight behaviour is an important attribute in understanding how wildlife, particularly birds, acquire drug-resistant flora. Based on these observations E. coli ST216 warrants further monitoring in bird populations in Australia and internationally. Furthermore, studies of the enterobacterial populations in urban-adapted and wild bird species using nonselective approaches is needed to improve understanding of lineages that colonise and persist in the avian gut. We also advise adopting a one health approach and investigating ST216 populations in silver gulls, humans and their surrounding environment to understand the transmission pathways and other features that influence pathogen evolution.

5. Conclusions

ST216 is a broad host range phylogroup A E. coli. Here we report the carriage of MDR E. coli ST216 by silver gulls on Five Islands near Wollongong, Australia harbouring diverse plasmids that carry multiple ARGs, VAGs and metal resistance genes. Most of the ST216 isolates were carbapenemase producers and carried blaIMP-4 on HI2-ST1 and HI2-ST3 plasmids. ARGs within HI2 plasmids were assembled in complex resistance regions together with metal resistance genes and multiple copies of IS elements including IS26 and IS26 derivative IS15DI. We report the spread of highly related IncHI2-ST1 plasmids between various bacterial hosts from different sources that include humans, domestic animals and wildlife in Australia. The recent increase of global ST216 reports isolated from different sources, expressing ARGs for critically important antibiotics and causing long lasting clinical outbreaks are concerning. To understand the transmission cycle of ARGs and MDR bacteria and its associated human risk, it is essential to adopt a one health approach that take into consideration all aspects of the ecological system with a focus on intermediate hosts (as gulls) that can act as vectors and sentinels for the spread of ARGs. Another consideration is the importance of interactions between different bacterial species mediated by mobile genetic elements such as HI2 plasmids and IS26 and its effect on the evolution and pathogenicity of these organisms.

Supplementary Materials

The following are available online at https://www.mdpi.com/2076-2607/9/3/567/s1, Figure S1: SNP-tree showing clonal relationship and genetic characteristics of Escherichia coli ST216 isolates from silver gulls at five islands and international related isolates. Figure S2: SNP-tree showing clonal relationship and genetic characteristics of Escherichia coli ST216 isolates from silver gulls at Five Islands. Figure S3: Heat maps showing the distribution of reference plasmids within sequenced short reads of Escherichia coli ST216 isolates from silver gulls at Five Islands. Figure S4-A: Schematic diagram of plasmid pCE1681-A (IncHI2-ST3). Figure S4-B: Schematic diagram of pCE1537-B (IncFIA) plasmid. Figure S4-C: BRIG comparison of IncX5 plasmid pCE681-E with similar plasmid sequence retrieved from GenBank. Figure S4-D: Schematic diagram of pCE1681-D (IncR) plasmid. Table S1: Characteristics of sequenced Escherichia coli ST216 isolates from silver gulls at Five Islands, Table S2: Characteristics of sequenced closed and complete plasmids in Escherichia coli ST216 isolates CE1537 and CE1681 from silver gulls at Five Islands.

Author Contributions

Conceptualisation and methodology: H.T., E.R.W., M.D., S.P.D.; validation: H.T., resources: H.T., E.R.W., I.B.; M.D., S.P.D.; formal analysis: H.T., investigation and data curation: H.T., E.R.W.; writing—original draft preparation: H.T.; writing—review and editing: H.T., E.R.W., M.D., S.P.D.; visualisation: H.T.; funding acquisition: I.B., M.D., S.P.D. All authors have read and agreed to the published version of the manuscript.

Funding

This work was funded by the Czech Science Foundation (18-23532S), by CEITEC 2020-Central European Institute of Technology (LQ1601) from the Czech Ministry of Education, by Charles University Research Fund PROGRES (project number Q39), partly by the project “Fighting Infectious Diseases” provided by the Ministry of Education Youth and Sports of the Czech Republic (CZ.02.1.01/0.0/0.0/16_019/0000787) and partly by the Internal Mobility Agency at the University of Veterinary and Pharmaceutical Sciences Brno (2019-FVHE-51). This study was also partly funded by the Medical Research Future Fund Frontier Health and Medical Research Program (MRFF75873) and the Australian Centre for Genomic Epidemiological Microbiology (AusGEM), a collaborative research partnership between the NSW Department of Primary Industries and the University of Technology Sydney.

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

Data presented in this study is available upon request. Part of this work was presented at the European Joint Programme (EJP) One Health conference in 2020 (talk number O:145).

Acknowledgments

We thank I. Literak and M. Havlicek for cooperation in the field and in sampling of silver gulls. We thank J. Lausova, D. Cervinkova, Katerina Chudejova and K. Anantanawat for their help in the laboratory. We also thank M. Cummins for his counsel in performing bioinformatics analysis. We acknowledge institutions which share their WGS data on Enterobase and other publicly available repositories.

Conflicts of Interest

The authors declare no conflict of interest.

References

  1. Vittecoq, M.; Godreuil, S.; Prugnolle, F.; Durand, P.; Brazier, L.; Renaud, N.; Arnal, A.; Aberkane, S.; Jean-Pierre, H.; Gauthier-Clerc, M.; et al. Antimicrobial resistance in wildlife. J. Appl. Ecol. 2016, 53, 519–529. [Google Scholar] [CrossRef] [Green Version]
  2. Eradhouani, H.; Esilva, N.; Epoeta, P.; Etorres, C.; Ecorreia, S.; Eigrejas, G. Potential impact of antimicrobial resistance in wildlife, environment and human health. Front. Microbiol. 2014, 5, 23. [Google Scholar] [CrossRef] [Green Version]
  3. Dolejska, M.; Literak, I. Wildlife Is Overlooked in the Epidemiology of Medically Important Antibiotic-Resistant Bacteria. Antimicrob. Agents Chemother. 2019, 63, e01167-19. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  4. Marcelino, V.R.; Wille, M.; Hurt, A.C.; González-Acuña, D.; Klaassen, M.; Schlub, T.E.; Eden, J.-S.; Shi, M.; Iredell, J.R.; Sorrell, T.C.; et al. Meta-transcriptomics reveals a diverse antibiotic resistance gene pool in avian microbiomes. BMC Biol. 2019, 17, 1–11. [Google Scholar] [CrossRef] [Green Version]
  5. Swift, B.M.; Bennett, M.; Waller, K.; Dodd, C.; Murray, A.; Gomes, R.L.; Humphreys, B.; Hobman, J.L.; Jones, M.A.; Whitlock, S.E.; et al. Anthropogenic environmental drivers of antimicrobial resistance in wildlife. Sci. Total Environ. 2019, 649, 12–20. [Google Scholar] [CrossRef] [PubMed]
  6. Dolejska, M.; Papagiannitsis, C.C. Plasmid-mediated resistance is going wild. Plasmid 2018, 99, 99–111. [Google Scholar] [CrossRef] [PubMed]
  7. Darwich, L.; Vidal, A.; Seminati, C.; Albamonte, A.; Casado, A.; López, F.; Molina-López, R.A.; Migura-Garcia, L. High prevalence and diversity of extended-spectrum β-lactamase and emergence of OXA-48 producing Enterobacterales in wildlife in Catalonia. PLoS ONE 2019, 14, e0210686. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  8. Oteo, J.; Mencía, A.; Bautista, V.; Pastor, N.; Lara, N.; González-González, F.; García-Peña, F.J.; Campos, J. Colonization with Enterobacteriaceae-Producing ESBLs, AmpCs, and OXA-48 in Wild Avian Species, Spain 2015–2016. Microb. Drug Resist. 2018, 24, 932–938. [Google Scholar] [CrossRef]
  9. Bouaziz, A.; Loucif, L.; Ayachi, A.; Guehaz, K.; Bendjama, E.; Rolain, J.-M. Migratory White Stork (Ciconia ciconia): A Potential Vector of the OXA-48-Producing Escherichia coli ST38 Clone in Algeria. Microb. Drug Resist. 2018, 24, 461–468. [Google Scholar] [CrossRef]
  10. Tarabai, H.; Valcek, A.; Jamborova, I.; Vazhov, S.V.; Karyakin, I.V.; Raab, R.; Literak, I.; Dolejska, M. Plasmid-Mediated mcr-1 Colistin Resistance in Escherichia coli from a Black Kite in Russia. Antimicrob. Agents Chemother. 2019, 63, e01266-19. [Google Scholar] [CrossRef] [Green Version]
  11. Sellera, F.P.; Fernandes, M.R.; Sartori, L.; Carvalho, M.P.N.; Esposito, F.; Nascimento, C.L.; Dutra, G.H.P.; Mamizuka, E.M.; Pérez-Chaparro, P.J.; McCulloch, J.A.; et al. Escherichia colicarrying IncX4 plasmid-mediatedmcr-1andblaCTX-Mgenes in infected migratory Magellanic penguins (Spheniscus magellanicus). J. Antimicrob. Chemother. 2016, 72, 1255–1256. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  12. Liakopoulos, A.; Mevius, D.J.; Olsen, B.; Bonnedahl, J. The colistin resistancemcr-1gene is going wild: Table 1. J. Antimicrob. Chemother. 2016, 71, 2335–2336. [Google Scholar] [CrossRef] [Green Version]
  13. Ahlstrom, C.A.; Ramey, A.M.; Woksepp, H.; Bonnedahl, J. Early emergence of mcr- 1-positive Enterobacteriaceae in gulls from Spain and Portugal. Environ. Microbiol. Rep. 2019, 11, 669–671. [Google Scholar] [CrossRef] [PubMed]
  14. Klemm, E.J.; Wong, V.K.; Dougan, G. Emergence of dominant multidrug-resistant bacterial clades: Lessons from history and whole-genome sequencing. Proc. Natl. Acad. Sci. USA. 2018, 115, 12872–12877. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  15. Venturini, C.; Hassan, K.A.; Chowdhury, P.R.; Paulsen, I.T.; Walker, M.J.; Djordjevic, S.P. Sequences of Two Related Multiple Antibiotic Resistance Virulence Plasmids Sharing a Unique IS26-Related Molecular Signature Isolated from Different Escherichia coli Pathotypes from Different Hosts. PLoS ONE 2013, 8, e78862. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  16. Mangat, C.S.; Bekal, S.; Irwin, R.J.; Mulvey, M.R. A Novel Hybrid Plasmid Carrying Multiple Antimicrobial Resistance and Virulence Genes in Salmonella enterica Serovar Dublin. Antimicrob. Agents Chemother. 2017, 61, e02601-16. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  17. McKinnon, J.; Chowdhury, P.R.; Djordjevic, S.P. Genomic analysis of multidrug-resistant Escherichia coli ST58 causing urosepsis. Int. J. Antimicrob. Agents 2018, 52, 430–435. [Google Scholar] [CrossRef] [Green Version]
  18. Wyrsch, E.R.; Hawkey, J.; Judd, L.M.; Haites, R.; Holt, K.E.; Djordjevic, S.P.; Billman-Jacobe, H. Z/I1 Hybrid Virulence Plasmids Carrying Antimicrobial Resistance genes in S. Typhimurium from Australian Food Animal Production. Microorganisms 2019, 7, 299. [Google Scholar] [CrossRef] [Green Version]
  19. Johnson, T.J.; Thorsness, J.L.; Anderson, C.P.; Lynne, A.M.; Foley, S.L.; Han, J.; Fricke, W.F.; McDermott, P.F.; White, D.G.; Khatri, M.; et al. Horizontal Gene Transfer of a ColV Plasmid Has Resulted in a Dominant Avian Clonal Type of Salmonella enterica Serovar Kentucky. PLoS ONE 2010, 5, e15524. [Google Scholar] [CrossRef] [PubMed]
  20. Venturini, C.; Beatson, S.A.; Djordjevic, S.P.; Walker, M.J. Multiple antibiotic resistance gene recruitment onto the enterohemorrhagic Escherichia coli virulence plasmid. FASEB J. 2009, 24, 1160–1166. [Google Scholar] [CrossRef]
  21. Wong, M.H.-Y.; Chan, E.W.-C.; Chen, S. IS26-mediated formation of a virulence and resistance plasmid in Salmonella Enteritidis. J. Antimicrob. Chemother. 2017, 72, 2750–2754. [Google Scholar] [CrossRef] [Green Version]
  22. Santos, A.C.; Santos, F.F.; Silva, R.M.; Gomes, T.A.T. Diversity of Hybrid- and Hetero-Pathogenic Escherichia coli and Their Potential Implication in More Severe Diseases. Front. Cell. Infect. Microbiol. 2020, 10, 339. [Google Scholar] [CrossRef] [PubMed]
  23. Croucher, N.J.; Klugman, K.P. The Emergence of Bacterial “Hopeful Monsters”. mBio 2014, 5, e01355-14. [Google Scholar] [CrossRef] [Green Version]
  24. Decraene, V.; Phan, H.T.T.; George, R.; Wyllie, D.H.; Akinremi, O.; Aiken, Z.; Cleary, P.; Dodgson, A.; Pankhurst, L.; Crook, D.W.; et al. A Large, Refractory Nosocomial Outbreak of Klebsiella pneumoniae Carbapenemase-Producing Escherichia coli Demonstrates Carbapenemase Gene Outbreaks Involving Sink Sites Require Novel Approaches to Infection Control. Antimicrob. Agents Chemother. 2018, 62, e01689-18. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  25. Baraniak, A.; Izdebski, R.; Fiett, J.; Herda, M.; Derde, L.P.G.; Bonten, M.J.M.; Adler, A.; Carmeli, Y.; Goossens, H.; Hryniewicz, W.; et al. KPC-Like Carbapenemase-Producing Enterobacteriaceae Colonizing Patients in Europe and Israel. Antimicrob. Agents Chemother. 2016, 60, 1912–1917. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  26. Hazen, T.H.; Zhao, L.; Boutin, M.A.; Stancil, A.; Robinson, G.; Harris, A.D.; Rasko, D.A.; Johnson, J.K. Comparative Genomics of an IncA/C Multidrug Resistance Plasmid from Escherichia coli and Klebsiella Isolates from Intensive Care Unit Patients and the Utility of Whole-Genome Sequencing in Health Care Settings. Antimicrob. Agents Chemother. 2014, 58, 4814–4825. [Google Scholar] [CrossRef] [Green Version]
  27. Manges, A.R.; Geum, H.M.; Guo, A.; Edens, T.J.; Fibke, C.D.; Pitout, J.D.D. Global Extraintestinal Pathogenic Escherichia coli (ExPEC) Lineages. Clin. Microbiol. Rev. 2019, 32, e00135-18. [Google Scholar] [CrossRef]
  28. Roer, L.; Overballe-Petersen, S.; Hansen, F.; Schønning, K.; Wang, M.; Røder, B.L.; Hansen, D.S.; Justesen, U.S.; Andersen, L.P.; Fulgsang-Damgaard, D.; et al. Escherichia coliSequence Type 410 Is Causing New International High-Risk Clones. mSphere 2018, 3, e00337-18. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  29. Zingali, T.; Chapman, T.A.; Webster, J.; Chowdhury, P.R.; Djordjevic, S.P. Genomic Characterisation of a Multiple Drug Resistant IncHI2 ST4 Plasmid in Escherichia coli ST744 in Australia. Microorganisms 2020, 8, 896. [Google Scholar] [CrossRef]
  30. Adler, A.; Baraniak, A.; Izdebski, R.; Fiett, J.; Salvia, A.; Samso, J.; Lawrence, C.; Solomon, J.; Paul, M.; Lerman, Y.; et al. A multinational study of colonization with extended spectrum β-lactamase-producing Enterobacteriaceae in healthcare personnel and family members of carrier patients hospitalized in rehabilitation centres. Clin. Microbiol. Infect. 2014, 20, O516–O523. [Google Scholar] [CrossRef] [Green Version]
  31. Del Bianco, F.; Morotti, M.; Pedna, M.; Farabegoli, P.; Sambri, V. Microbiological surveillance of plasmid mediated colistin resistance in human Enterobacteriaceae isolates in Romagna (Northern Italy): August 2016–July 2017. Int. J. Infect. Dis. 2018, 69, 96–98. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  32. Cheruvanky, A.; Stoesser, N.; Sheppard, A.E.; Crook, D.W.; Hoffman, P.S.; Weddle, E.; Carroll, J.; Sifri, C.D.; Chai, W.; Barry, K.; et al. Enhanced Klebsiella pneumoniae Carbapenemase Expression from a Novel Tn4401 Deletion. Antimicrob. Agents Chemother. 2017, 61, e00025-17. [Google Scholar] [CrossRef] [Green Version]
  33. Piedra-Carrasco, N.; Fàbrega, A.; Calero-Cáceres, W.; Cornejo-Sánchez, T.; Brown-Jaque, M.; Mir-Cros, A.; Muniesa, M.; González-López, J.J. Carbapenemase-producing enterobacteriaceae recovered from a Spanish river ecosystem. PLoS ONE 2017, 12, e0175246. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  34. Dolejska, M.; Masarikova, M.; Dobiasova, H.; Jamborova, I.; Karpiskova, R.; Havlicek, M.; Carlile, N.; Priddel, D.; Cizek, A.; Literak, I. High prevalence ofSalmonellaand IMP-4-producing Enterobacteriaceae in the silver gull on Five Islands, Australia. J. Antimicrob. Chemother. 2016, 71, 63–70. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  35. Nesporova, K.; Wyrsch, E.R.; Valcek, A.; Bitar, I.; Chaw, K.; Harris, P.; Hrabak, J.; Literak, I.; Djordjevic, S.P.; Dolejska, M. Escherichia coli Sequence Type 457 Is an Emerging Extended-Spectrum-β-Lactam-Resistant Lineage with Reservoirs in Wildlife and Food-Producing Animals. Antimicrob. Agents Chemother. 2020, 65, e01118-20. [Google Scholar] [CrossRef]
  36. Cummins, M.L.; Sanderson-Smith, M.; Newton, P.; Carlile, N.; Phalen, D.N.; Maute, K.; Monahan, L.G.; Hoye, B.J.; Djordjevic, S.P. Whole-Genome Sequence Analysis of an Extensively Drug-Resistant Salmonella enterica Serovar Agona Isolate from an Australian Silver Gull (Chroicocephalus novaehollandiae) Reveals the Acquisition of Multidrug Resistance Plasmids. mSphere 2020, 5, e00743-20. [Google Scholar] [CrossRef]
  37. Dolejska, M.; Cizek, A.; Literak, I. High prevalence of antimicrobial-resistant genes and integrons in Escherichia coli isolates from Black-headed Gulls in the Czech Republic. J. Appl. Microbiol. 2007, 103, 11–19. [Google Scholar] [CrossRef]
  38. Cole, D.; Drum, D.J.; Stallknecht, D.E.; White, D.G.; Lee, M.D.; Ayers, S.; Sobsey, M.; Maurer, J.J. Free-living Canada Geese and Antimicrobial Resistance. Emerg. Infect. Dis. 2005, 11, 935–938. [Google Scholar] [CrossRef]
  39. Seeman, T. Shovill: Faster SPAdes Assembly of Illumina Reads (v0.9.0). Available online: https://github.com/tseemann/shovill (accessed on 28 April 2020).
  40. Chin, C.-S.; Alexander, D.H.; Marks, P.; Klammer, A.A.; Drake, J.; Heiner, C.; Clum, A.; Copeland, A.; Huddleston, J.; Eichler, E.E.; et al. Nonhybrid, finished microbial genome assemblies from long-read SMRT sequencing data. Nat. Methods 2013, 10, 563–569. [Google Scholar] [CrossRef]
  41. Zhou, Z.; Alikhan, N.-F.; Mohamed, K.; Fan, Y.; Achtman, M.; the Agama Study Group; Brown, D.; Chattaway, M.; Dallman, T.; Delahay, R.; et al. The EnteroBase user’s guide, with case studies on Salmonella transmissions, Yersinia pestis phylogeny, and Escherichia core genomic diversity. Genome Res. 2020, 30, 138–152. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  42. Alcock, B.P.; Raphenya, A.R.; Lau, T.T.Y.; Tsang, K.K.; Bouchard, M.; Edalatmand, A.; Huynh, W.; Nguyen, A.-L.V.; Cheng, A.A.; Liu, S.; et al. CARD 2020: Antibiotic resistome surveillance with the comprehensive antibiotic resistance database. Nucleic Acids Res. 2020, 48, D517–D525. [Google Scholar] [CrossRef]
  43. Chen, L.; Zheng, D.; Liu, B.; Yang, J.; Jin, Q. VFDB 2016: Hierarchical and refined dataset for big data analysis—10 years on. Nucleic Acids Res. 2016, 44, D694–D697. [Google Scholar] [CrossRef] [PubMed]
  44. Brettin, T.; Davis, J.J.; Disz, T.; Edwards, R.A.; Gerdes, S.; Olsen, G.J.; Olson, R.J.; Overbeek, R.; Parrello, B.; Pusch, G.D.; et al. RASTtk: A modular and extensible implementation of the RAST algorithm for building custom annotation pipelines and annotating batches of genomes. Sci. Rep. 2015, 5, srep08365. [Google Scholar] [CrossRef] [Green Version]
  45. Siguier, P. ISfinder: The reference centre for bacterial insertion sequences. Nucleic Acids Res. 2006, 34 (Suppl. 1), D32–D36. [Google Scholar] [CrossRef] [Green Version]
  46. Arndt, D.; Grant, J.R.; Marcu, A.; Sajed, T.; Pon, A.; Liang, Y.; Wishart, D.S. PHASTER: A better, faster version of the PHAST phage search tool. Nucleic Acids Res. 2016, 44, W16–W21. [Google Scholar] [CrossRef] [Green Version]
  47. EUCAST. Antimicrobial Susceptibility Testing. EUCAST Disk Diffusion Method. Available online: http://www.eucast.org/fileadmin/src/media/PDFs/EUCAST_files/Disk_test_documents/2019_manuals/Manual_v_7.0_EUCAST_Disk_Test_2019.pdf (accessed on 18 December 2019).
  48. EUCAST. Breakpoint Tables for Interpretation of MICs and Zone Diameters. Available online: http://www.eucast.org/fileadmin/src/media/PDFs/EUCAST_files/Breakpoint_tables/v_9.0_Breakpoint_Tables.pdf (accessed on 19 December 2019).
  49. CLSI. Performance Standards for Antimicrobial Susceptibility Testing. In CLSI Supplement M100, 27th ed.; Clinical and Laboratory Standards Institute: Wayne, PA, USA, 2017; Available online: http://file.qums.ac.ir/repository/mmrc/clsi%202017.pdf (accessed on 19 December 2019).
  50. Jouy, E.; Haenni, M.; Le Devendec, L.; Le Roux, A.; Châtre, P.; Madec, J.-Y.; Kempf, I. Improvement in routine detection of colistin resistance in E. coli isolated in veterinary diagnostic laboratories. J. Microbiol. Methods 2017, 132, 125–127. [Google Scholar] [CrossRef] [PubMed]
  51. Rotova, V.; Papagiannitsis, C.C.; Skalova, A.; Chudejova, K.; Hrabak, J. Comparison of imipenem and meropenem antibiotics for the MALDI-TOF MS detection of carbapenemase activity. J. Microbiol. Methods 2017, 137, 30–33. [Google Scholar] [CrossRef]
  52. Magiorakos, A.-P.; Srinivasan, A.; Carey, R.B.; Carmeli, Y.; Falagas, M.E.; Giske, C.G.; Harbarth, S.; Hindler, J.F.; Kahlmeter, G.; Olsson-Liljequist, B.; et al. Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria: An international expert proposal for interim standard definitions for acquired resistance. Clin. Microbiol. Infect. 2012, 18, 268–281. [Google Scholar] [CrossRef] [Green Version]
  53. Kaas, R.S.; Leekitcharoenphon, P.; Aarestrup, F.M.; Lund, O. Solving the Problem of Comparing Whole Bacterial Genomes across Different Sequencing Platforms. PLoS ONE 2014, 9, e104984. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  54. Letunic, I.; Bork, P. Interactive Tree of Life (iTOL) v4: Recent updates and new developments. Nucleic Acids Res. 2019, 47, W256–W259. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  55. Darling, A.E.; Mau, B.; Perna, N.T. progressiveMauve: Multiple Genome Alignment with Gene Gain, Loss and Rearrangement. PLoS ONE 2010, 5, e11147. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  56. Cummins, M.L.; Reid, C.J.; Chowdhury, P.R.; Bushell, R.N.; Esbert, N.; Tivendale, K.A.; Noormohammadi, A.H.; Islam, S.; Marenda, M.S.; Browning, G.F.; et al. Whole genome sequence analysis of Australian avian pathogenic Escherichia coli that carry the class 1 integrase gene. Microb. Genom. 2019, 5, e000250. [Google Scholar] [CrossRef] [PubMed]
  57. Alikhan, N.-F.; Petty, N.K.; Ben Zakour, N.L.; Beatson, S.A. BLAST Ring Image Generator (BRIG): Simple prokaryote genome comparisons. BMC Genom. 2011, 12, 402. [Google Scholar] [CrossRef] [Green Version]
  58. Sullivan, M.J.; Petty, N.K.; Beatson, S.A. Easyfig: A genome comparison visualizer. Bioinformatics 2011, 27, 1009–1010. [Google Scholar] [CrossRef] [PubMed]
  59. Carattoli, A.; Bertini, A.; Villa, L.; Falbo, V.; Hopkins, K.L.; Threlfall, E.J. Identification of plasmids by PCR-based replicon typing. J. Microbiol. Methods 2005, 63, 219–228. [Google Scholar] [CrossRef]
  60. Valcek, A.; Overballe-Petersen, S.; Hansen, F.; Dolejska, M.; Hasman, H. Complete Genome Sequence of Escherichia coli MT102, a Plasmid-Free Recipient Resistant to Rifampin, Azide, and Streptomycin, Used in Conjugation Experiments. Microbiol. Resour. Announc. 2019, 8, e00383-19. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  61. Everett, M.J.; Jin, Y.F.; Ricci, V.; Piddock, L.J. Contributions of individual mechanisms to fluoroquinolone resistance in 36 Escherichia coli strains isolated from humans and animals. Antimicrob. Agents Chemother. 1996, 40, 2380–2386. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  62. Pong, C.H.; Harmer, C.J.; Ataide, S.F.; Hall, R.M. An IS26variant with enhanced activity. FEMS Microbiol. Lett. 2019, 366, fnz031. [Google Scholar] [CrossRef]
  63. Reid, C.J.; Chowdhury, P.R.; Djordjevic, S.P. Tn6026 and Tn6029 are found in complex resistance regions mobilised by diverse plasmids and chromosomal islands in multiple antibiotic resistant Enterobacteriaceae. Plasmid 2015, 80, 127–137. [Google Scholar] [CrossRef]
  64. Wyrsch, E.R.; Reid, C.J.; DeMaere, M.Z.; Liu, M.Y.; Chapman, T.A.; Chowdhury, P.R.; Djordjevic, S.P. Complete Sequences of Multiple-Drug Resistant IncHI2 ST3 Plasmids in Escherichia coli of Porcine Origin in Australia. Front. Sustain. Food Syst. 2019, 3, 18. [Google Scholar] [CrossRef]
  65. Cascales, E.; Buchanan, S.K.; Duché, D.; Kleanthous, C.; Lloubès, R.; Postle, K.; Riley, M.; Slatin, S.; Cavard, D. Colicin Biology. Microbiol. Mol. Biol. Rev. 2007, 71, 158–229. [Google Scholar] [CrossRef] [Green Version]
  66. Gordon, A.K.; Phan, H.T.T.; Lipworth, S.I.; Cheong, E.; Gottlieb, T.; George, S.; Peto, T.E.A.; Mathers, A.J.; Walker, A.S.; Crook, D.W.; et al. Genomic dynamics of species and mobile genetic elements in a prolonged blaIMP-4-associated carbapenemase outbreak in an Australian hospital. J. Antimicrob. Chemother. 2020, 75, 873–882. [Google Scholar] [CrossRef]
  67. Roberts, L.W.; Harris, P.N.A.; Forde, B.M.; Ben Zakour, N.L.; Catchpoole, E.; Stanton-Cook, M.; Phan, M.-D.; Sidjabat, H.E.; Bergh, H.; Heney, C.; et al. Integrating multiple genomic technologies to investigate an outbreak of carbapenemase-producing Enterobacter hormaechei. Nat. Commun. 2020, 11, 1–11. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  68. Abraham, S.; O’Dea, M.; Trott, D.J.; Abraham, R.J.; Hughes, D.; Pang, S.; McKew, G.; Cheong, E.Y.L.; Merlino, J.; Saputra, S.; et al. Isolation and plasmid characterization of carbapenemase (IMP-4) producing Salmonella enterica Typhimurium from cats. Sci. Rep. 2016, 6, 35527. [Google Scholar] [CrossRef] [PubMed]
  69. Dolejska, M.; Papagiannitsis, C.C.; Medvecky, M.; Davidova-Gerzova, L.; Valcek, A. Characterization of the Complete Nucleotide Sequences of IMP-4-Encoding Plasmids, Belonging to Diverse Inc Families, Recovered from Enterobacteriaceae Isolates of Wildlife Origin. Antimicrob. Agents Chemother. 2018, 62, e02434-17. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  70. Wang, J.; Ma, Z.-B.; Zeng, Z.-L.; Yang, X.-W.; Huang, Y.; Liu, J.-H. The role of wildlife (wild birds) in the global transmission of antimicrobial resistance genes. Zool. Res. 2017, 38, 55–80. [Google Scholar] [CrossRef] [Green Version]
  71. Wu, J.; Huang, Y.; Rao, D.; Zhang, Y.; Yang, K. Evidence for Environmental Dissemination of Antibiotic Resistance Mediated by Wild Birds. Front. Microbiol. 2018, 9, 745. [Google Scholar] [CrossRef]
  72. Mukerji, S.; Stegger, M.; Truswell, A.V.; Laird, T.; Jordan, D.; Abraham, R.J.; Harb, A.; Barton, M.; O’Dea, M.; Abraham, S. Resistance to critically important antimicrobials in Australian silver gulls (Chroicocephalus novaehollandiae) and evidence of anthropogenic origins. J. Antimicrob. Chemother. 2019, 74, 2566–2574. [Google Scholar] [CrossRef]
  73. Harmer, C.J.; Moran, R.A.; Hall, R.M. Movement of IS26-Associated Antibiotic Resistance Genes Occurs via a Translocatable Unit That Includes a Single IS26 and Preferentially Inserts Adjacent to Another IS26. mBio 2014, 5, e01801-14. [Google Scholar] [CrossRef] [Green Version]
  74. Wan, M.; Gao, X.; Lv, L.; Cai, Z.; Liu, J.H. IS26 mediate the acquisition of tigecycline resistance gene cluster tmexCD1-toprJ1 by IncHI1B-FIB plasmids in Klebsiella pneumoniae and Klebsie lla quasipneumoniae from food market sewage. Antimicrob. Agents Chemother. 2020, 65, e02178-20. [Google Scholar] [CrossRef]
  75. Cain, A.K.; Liu, X.; Djordjevic, S.P.; Hall, R.M. Transposons Related to Tn1696in IncHI2 Plasmids in Multiply Antibiotic ResistantSalmonella entericaSerovar Typhimurium from Australian Animals. Microb. Drug Resist. 2010, 16, 197–202. [Google Scholar] [CrossRef]
  76. Hastak, P.; Cummins, M.L.; Gottlieb, T.; Cheong, E.; Merlino, J.; Myers, G.S.A.; Djordjevic, S.P.; Chowdhury, P.R. Genomic profiling of Escherichia coli isolates from bacteraemia patients: A 3-year cohort study of isolates collected at a Sydney teaching hospital. Microb. Genom. 2020, 6, e000371. [Google Scholar] [CrossRef]
  77. Reid, C.J.; Wyrsch, E.R.; Chowdhury, P.R.; Zingali, T.; Liu, M.; Darling, A.E.; Chapman, T.A.; Djordjevic, S.P. Porcine commensal Escherichia coli: A reservoir for Class 1 Integrons Associated with IS26. Microb. Genom. 2017, 3, e000143. [Google Scholar] [CrossRef] [PubMed]
  78. Dawes, F.E.; Kuzevski, A.; Bettelheim, K.A.; Hornitzky, M.A.; Djordjevic, S.P.; Walker, M.J. Distribution of Class 1 Integrons with IS26-Mediated Deletions in Their 3′-Conserved Segments in Escherichia coli of Human and Animal Origin. PLoS ONE 2010, 5, e12754. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  79. Li, D.; Reid, C.J.; Kudinha, T.; Jarocki, V.M.; Djordjevic, S.P. Genomic analysis of trimethoprim-resistant extraintestinal pathogenic Escherichia coli and recurrent urinary tract infections. Microb. Genom. 2020, 6, mgen000475. [Google Scholar] [CrossRef]
  80. García, V.; García, P.; Rodríguez, I.; Rodicio, R.; Rodicio, M.D.R.R. The role of IS 26 in evolution of a derivative of the virulence plasmid of Salmonella enterica serovar Enteritidis which confers multiple drug resistance. Infect. Genet. Evol. 2016, 45, 246–249. [Google Scholar] [CrossRef] [PubMed]
  81. Porse, A.; Schønning, K.; Munck, C.; Sommer, M.O. Survival and Evolution of a Large Multidrug Resistance Plasmid in New Clinical Bacterial Hosts. Mol. Biol. Evol. 2016, 33, 2860–2873. [Google Scholar] [CrossRef]
  82. Yang, X.; Ye, L.; Li, Y.; Chan, E.W.-C.; Zhang, R.; Chen, S. Identification of a Chromosomal Integrated DNA Fragment Containing the rmpA2 and iucABCDiutA Virulence Genes in Klebsiella pneumoniae. mSphere 2020, 5, e01179-20. [Google Scholar] [CrossRef] [PubMed]
  83. Carattoli, A. Plasmids in Gram negatives: Molecular typing of resistance plasmids. Int. J. Med. Microbiol. 2011, 301, 654–658. [Google Scholar] [CrossRef] [PubMed]
  84. Ravi, A.; Valdés-Varela, L.; Gueimonde, M.; Rudi, K. Transmission and persistence of IncF conjugative plasmids in the gut microbiota of full-term infants. FEMS Microbiol. Ecol. 2018, 94, 94. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  85. Girón, J.A.; Torres, A.G.; Freer, E.; Kaper, J.B. The flagella of enteropathogenic Escherichia coli mediate adherence to epithelial cells. Mol. Microbiol. 2002, 44, 361–379. [Google Scholar] [CrossRef] [PubMed]
  86. Luck, S.N.; Badea, L.; Bennett-Wood, V.; Robins-Browne, R.; Hartland, E.L. Contribution of FliC to Epithelial Cell Invasion by Enterohemorrhagic Escherichia coli O113:H21. Infect. Immun. 2006, 74, 6999–7004. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  87. He, Y.; Xu, T.; Fossheim, L.E.; Zhang, X.-H. FliC, a Flagellin Protein, Is Essential for the Growth and Virulence of Fish Pathogen Edwardsiella tarda. PLoS ONE 2012, 7, e45070. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  88. Eporcheron, G.; Egarenaux, A.; Eproulx, J.; Esabri, M.; Dozois, C.M. Iron, copper, zinc, and manganese transport and regulation in pathogenic Enterobacteria: Correlations between strains, site of infection and the relative importance of the different metal transport systems for virulence. Front. Cell. Infect. Microbiol. 2013, 3, 90. [Google Scholar] [CrossRef] [Green Version]
  89. Pal, C.; Asiani, K.; Arya, S.; Rensing, C.; Stekel, D.J.; Larsson, D.J.; Hobman, J.L. Metal Resistance and Its Association With Antibiotic Resistance. Adv. Microb. Physiol. 2017, 70, 261–313. [Google Scholar] [CrossRef] [PubMed]
  90. Smith, G.C.; Carlile, N. Food and Feeding Ecology of Breeding Silver Gulls (Larus novaehollandiae) in Urban Australia. Colon. Waterbirds 1993, 16, 9. [Google Scholar] [CrossRef]
  91. Jackson, R.W.; Johnson, L.J.; Clarke, S.R.; Arnold, D.L. Bacterial pathogen evolution: Breaking news. Trends Genet. 2011, 27, 32–40. [Google Scholar] [CrossRef] [PubMed]
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Figure 1. Clonal relationship of Escherichia coli ST216 isolates from silver gulls (n = 22) at Five Islands and international related isolates (n = 100).
Figure 1. Clonal relationship of Escherichia coli ST216 isolates from silver gulls (n = 22) at Five Islands and international related isolates (n = 100).
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Figure 2. BRIG comparison of colicin E7 positive Col156 plasmid pCE681-C with similar plasmid sequences retrieved from GenBank. In the key, HP: hypothetical protein.
Figure 2. BRIG comparison of colicin E7 positive Col156 plasmid pCE681-C with similar plasmid sequences retrieved from GenBank. In the key, HP: hypothetical protein.
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Figure 3. Comparison of IS26-composite transposon region and its background within IncHI2-ST1 plasmids from wildlife (pCE1537-A), companion animal (pIMP4-SEM1) and humans (pMS7884a and pC15_001). In the key, ARG: antibiotic-resistance gene and VAG/MRG: virulence associated gene/metal resistance gene.
Figure 3. Comparison of IS26-composite transposon region and its background within IncHI2-ST1 plasmids from wildlife (pCE1537-A), companion animal (pIMP4-SEM1) and humans (pMS7884a and pC15_001). In the key, ARG: antibiotic-resistance gene and VAG/MRG: virulence associated gene/metal resistance gene.
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Figure 4. BRIG comparison of blaIMP-4-positive IncHI2-ST1 plasmid pCE1537-A with similar plasmid sequences retrieved from GenBank. In the key, ARG: antibiotic-resistance gene, VAG/MRG: virulence associated gene/metal resistance gene, MGE: mobile genetic element and HP: hypothetical protein.
Figure 4. BRIG comparison of blaIMP-4-positive IncHI2-ST1 plasmid pCE1537-A with similar plasmid sequences retrieved from GenBank. In the key, ARG: antibiotic-resistance gene, VAG/MRG: virulence associated gene/metal resistance gene, MGE: mobile genetic element and HP: hypothetical protein.
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Figure 5. Heat maps showing the distribution of reference plasmids within sequenced short reads of E. coli ST216 isolates from silver gulls at Five Islands. Blue colour indicates the coverage profile of each short read with respect to the reference plasmid. Reference plasmids: (A) pCE1537-A (IncHI2-ST1) and (B) pCE1681-A (IncHI2-ST3).
Figure 5. Heat maps showing the distribution of reference plasmids within sequenced short reads of E. coli ST216 isolates from silver gulls at Five Islands. Blue colour indicates the coverage profile of each short read with respect to the reference plasmid. Reference plasmids: (A) pCE1537-A (IncHI2-ST1) and (B) pCE1681-A (IncHI2-ST3).
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Figure 6. BRIG comparison of qnrS1 positive IncFIB(K) plasmid pCE681-B with similar plasmid sequences retrieved from GenBank. In the key, ARG: antibiotic-resistance gene, MRG: metal resistance gene, MGE: mobile genetic element and HP: hypothetical protein.
Figure 6. BRIG comparison of qnrS1 positive IncFIB(K) plasmid pCE681-B with similar plasmid sequences retrieved from GenBank. In the key, ARG: antibiotic-resistance gene, MRG: metal resistance gene, MGE: mobile genetic element and HP: hypothetical protein.
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Table
Table 1. Escherichia coli ST216 short read and long read sequences, metadata and quality control statistics.
Table 1. Escherichia coli ST216 short read and long read sequences, metadata and quality control statistics.
Sequence IDSequence TypeEnterobase Barcode No.GenBank Accession No.CoverageTotal SequencesPoor Quality SequencesSequence Length (bp)GC%
1556m1SRESC_QA8784AAJAEUYL00000000095×1,226,163015151
CE1586SRESC_QA8782AAJAEUYK00000000072×1,588,914015151
1548R1SRESC_QA8786AAJAEUXR00000000079×1,386,478015151
1552m1SRESC_QA8785AAJAEUXS00000000083×1,425,868015151
CE1585SRESC_RA0975AAJAEUXT000000000143×2,366,204015151
1587RSRESC_RA0998AAJAEUXU00000000084×1,440,780015150
CE1724SRESC_RA0997AAJAEUXV00000000076×1,286,453015151
CE1681SRESC_RA0995AAJAEUXW00000000073×1,367,751015151
1605m2SRESC_RA0996AAJAEUXX00000000081×1,354,070015151
1660m1SRESC_QA8910AJAEUXY00000000077×1,298,542015151
CE1700SRESC_QA8911AAJAEUXZ00000000074×1,254,843015151
1537HSRESC_QA8912AAJAEUYA000000000127×2,206,779015152
1720HSRESC_QA8787AAJAEUYJ00000000016×221,227015151
1726HSRESC_QA8788AAJAEUYI00000000058×974,309015151
1560HSRESC_QA8790AAJAEUYH00000000061×1,042,416015151
1561HSRESC_QA8789AAJAEUYG00000000043×729,472015151
1539HSRESC_QA8791AAJAEUYF000000000106×1,912,588015151
1585HSRESC_QA8795AAJAEUYE000000000499×9,553,397015151
CE1539SRESC_QA8792AAJAEUYD000000000161×2,911,482015151
CE1580SRESC_QA8794AAJAEUYC000000000358×7,231,680015152
CE1538BSRESC_QA8793AAJAEUYB000000000123×2,115,704015151
CE1537GLRNDJABBCF000000000203×462,216051-19780250
CE1537PLRNDND317×98,607050-10399146
SR: short read, GLR: genome long read, PLR: plasmids long read and ND: not defined.
Table
Table 2. Characteristics of plasmids used for comparison with plasmids pCE1537-A, pCE1681-B, pCE1681-E, and pCE1681-C.
Table 2. Characteristics of plasmids used for comparison with plasmids pCE1537-A, pCE1681-B, pCE1681-E, and pCE1681-C.
Plasmid ID Comparison PlasmidGenBank Accession no.Coverage (%)Identity (%) RepliconSpeciesSourceCountry RegionYear
pCE1537-ApAUSMDU8141-1CP022696.110099.77IncHI2-ST1Citrobacter farmeriHumanAustraliaVictoria2015
pC15_001CP042489.19999.31IncHI2-ST1Enterobacter hormaecheiHumanAustraliaSydney2009
pIMP4-SEM1KX810825.19699.73IncHI2-ST1Salmonella entericaCatAustraliaNDND
pMS7884ACP022533.19199.71IncHI2-ST1Enterobacter hormaecheiHumanAustraliaBrisbane 2015
pCE1681-B CP020344.1CP020344.19399.98IncFIB(K)Shigella flexneriHumanChinaHangzhou2004
pCFSAN061762CP042902.19199.99IncFIB(K)Escherichia coliRaw milk Egypt ND2016
pFZ11KY051550.19199.99IncFIB(K)Escherichia coliHumanChinaFujianND
pCFSAN061763CP042900.19199.99IncFIB(K)Escherichia coliRaw milk Egypt ND2016
pCFSAN061768CP042974.191100IncFIB(K)Escherichia coliRaw milk Egypt ND2016
pCE1681-CpCE1537-CMT162141.110099.83Col156Escherichia coliSilver gullAustraliaSydney2012
ColE7-K317KJ470776.110099.54Col156Escherichia coliND PakistanNDND
pECAZ146_5CP018986.110099.46Col156Escherichia coliHuman ItalyPisa2012
pCE1681-EpCE1537-CMT162141.110099.83Col156 Escherichia coliSilver gullAustraliaSydney2012
pEc1677MG516910.186100IncX5Escherichia coliSilver gullAustralia Sydney2012
ND: Not defined.
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Tarabai, H.; Wyrsch, E.R.; Bitar, I.; Dolejska, M.; Djordjevic, S.P. Epidemic HI2 Plasmids Mobilising the Carbapenemase Gene blaIMP-4 in Australian Clinical Samples Identified in Multiple Sublineages of Escherichia coli ST216 Colonising Silver Gulls. Microorganisms 2021, 9, 567. https://doi.org/10.3390/microorganisms9030567

AMA Style

Tarabai H, Wyrsch ER, Bitar I, Dolejska M, Djordjevic SP. Epidemic HI2 Plasmids Mobilising the Carbapenemase Gene blaIMP-4 in Australian Clinical Samples Identified in Multiple Sublineages of Escherichia coli ST216 Colonising Silver Gulls. Microorganisms. 2021; 9(3):567. https://doi.org/10.3390/microorganisms9030567

Chicago/Turabian Style

Tarabai, Hassan, Ethan R. Wyrsch, Ibrahim Bitar, Monika Dolejska, and Steven P. Djordjevic. 2021. "Epidemic HI2 Plasmids Mobilising the Carbapenemase Gene blaIMP-4 in Australian Clinical Samples Identified in Multiple Sublineages of Escherichia coli ST216 Colonising Silver Gulls" Microorganisms 9, no. 3: 567. https://doi.org/10.3390/microorganisms9030567

APA Style

Tarabai, H., Wyrsch, E. R., Bitar, I., Dolejska, M., & Djordjevic, S. P. (2021). Epidemic HI2 Plasmids Mobilising the Carbapenemase Gene blaIMP-4 in Australian Clinical Samples Identified in Multiple Sublineages of Escherichia coli ST216 Colonising Silver Gulls. Microorganisms, 9(3), 567. https://doi.org/10.3390/microorganisms9030567

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