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Microorganisms
Volume 9
Issue 3
10.3390/microorganisms9030567
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Article

Epidemic HI2 Plasmids Mobilising the Carbapenemase Gene blaIMP-4 in Australian Clinical Samples Identified in Multiple Sublineages of Escherichia coli ST216 Colonising Silver Gulls

by
Hassan Tarabai
1,2,
Ethan R. Wyrsch
3,
Ibrahim Bitar
2,4,
Monika Dolejska
1,2,4,5,* and
Steven P. Djordjevic
3,*
1
Department of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Brno 612 42, Czech Republic
2
Central European Institute of Technology (CEITEC), University of Veterinary and Pharmaceutical Sciences Brno, Brno 612 42, Czech Republic
3
iThree Institute, University of Technology Sydney, Sydney, NSW 2007, Australia
4
Biomedical Center, Faculty of medicine in Pilsen, Charles University, Pilsen 323 00, Czech Republic
5
Department of Clinical Microbiology and Immunology, Institute of Laboratory Medicine, The University Hospital Brno, Brno 625 00, Czech Republic
*
Authors to whom correspondence should be addressed.
Academic Editors: Raffaele Zarrilli, Tommaso Giani and Rémy A. Bonnin
Microorganisms 2021, 9(3), 567; https://doi.org/10.3390/microorganisms9030567
Received: 31 January 2021 / Revised: 4 March 2021 / Accepted: 5 March 2021 / Published: 10 March 2021
(This article belongs to the Special Issue Molecular Epidemiology of Antimicrobial Resistance)
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Abstract

Escherichia coli ST216, including those that carry blaKPC-2, blaFOX-5, blaCTX-M-15 and mcr-1, have been linked to wild and urban-adapted birds and the colonisation of hospital environments causing recalcitrant, carbapenem-resistant human infections. Here we sequenced 22 multiple-drug resistant ST216 isolates from Australian silver gull chicks sampled from Five Islands, of which 21 carried nine or more antibiotic resistance genes including blaIMP-4 (n = 21), blaTEM-1b (n = 21), aac(3)-IId (n = 20), mph(A) (n = 20), catB3 (n = 20), sul1 (n = 20), aph(3”)-Ib (n = 18) and aph(6)-Id (n = 18) on FIB(K) (n = 20), HI2-ST1 (n = 11) and HI2-ST3 (n = 10) plasmids. We show that (i) all HI2 plasmids harbour blaIMP-4 in resistance regions containing In809 flanked by IS26 (HI2-ST1) or IS15DI (HI2-ST3) and diverse metal resistance genes; (ii) HI2-ST1 plasmids are highly related to plasmids reported in diverse Enterobacteriaceae sourced from humans, companion animals and wildlife; (iii) HI2 were a feature of the Australian gull isolates and were not observed in international ST216 isolates. Phylogenetic analyses identified close relationships between ST216 from Australian gull and clinical isolates from overseas. E. coli ST216 from Australian gulls harbour HI2 plasmids encoding resistance to clinically important antibiotics and metals. Our studies underscore the importance of adopting a one health approach to AMR and pathogen surveillance. View Full-Text
Keywords: anthropogenic pollution; ST216; Australian silver gull; urban birds; wildlife; whole genome sequencing; Chroicocephalus novaehollandiae anthropogenic pollution; ST216; Australian silver gull; urban birds; wildlife; whole genome sequencing; Chroicocephalus novaehollandiae
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MDPI and ACS Style

Tarabai, H.; Wyrsch, E.R.; Bitar, I.; Dolejska, M.; Djordjevic, S.P. Epidemic HI2 Plasmids Mobilising the Carbapenemase Gene blaIMP-4 in Australian Clinical Samples Identified in Multiple Sublineages of Escherichia coli ST216 Colonising Silver Gulls. Microorganisms 2021, 9, 567. https://doi.org/10.3390/microorganisms9030567

AMA Style

Tarabai H, Wyrsch ER, Bitar I, Dolejska M, Djordjevic SP. Epidemic HI2 Plasmids Mobilising the Carbapenemase Gene blaIMP-4 in Australian Clinical Samples Identified in Multiple Sublineages of Escherichia coli ST216 Colonising Silver Gulls. Microorganisms. 2021; 9(3):567. https://doi.org/10.3390/microorganisms9030567

Chicago/Turabian Style

Tarabai, Hassan, Ethan R. Wyrsch, Ibrahim Bitar, Monika Dolejska, and Steven P. Djordjevic. 2021. "Epidemic HI2 Plasmids Mobilising the Carbapenemase Gene blaIMP-4 in Australian Clinical Samples Identified in Multiple Sublineages of Escherichia coli ST216 Colonising Silver Gulls" Microorganisms 9, no. 3: 567. https://doi.org/10.3390/microorganisms9030567

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