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Article

Nontuberculous Mycobacteria Prevalence in Bats’ Guano from Caves and Attics of Buildings Studied by Culture and qPCR Examinations

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Faculty of Regional Development and International Studies, Mendel University in Brno, Tr. Generala Piky 7, 613 00 Brno, Czech Republic
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Public Health Institute Ostrava, Partyzanske Nam. 7, 702 00 Ostrava, Czech Republic
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Faculty of AgriSciences, Mendel University in Brno, Zemedelska 1/1665, 613 00 Brno, Czech Republic
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Institute of Vertebrate Biology of the Czech Academy of Sciences, v.v.i., Kvetna 8, 603 65 Brno, Czech Republic
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Faculty of Veterinary Hygiene and Ecology, University of Veterinary Sciences Brno, Palackeho Tr. 1946/1, 612 42 Brno, Czech Republic
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Museum Blanenska, Zamek 1/1, 678 01 Blansko, Czech Republic
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Central Bohemian Archaeological Heritage Institute, Nad Olsinami 448/3, 100 00 Prague, Czech Republic
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Veterinary Research Institute, v.v.i., Hudcova 70, 621 00 Brno, Czech Republic
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Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Bundoora, Melbourne, VIC 3086, Australia
*
Author to whom correspondence should be addressed.
Academic Editor: Hiroyuki Yamada
Microorganisms 2021, 9(11), 2236; https://doi.org/10.3390/microorganisms9112236
Received: 14 September 2021 / Revised: 24 October 2021 / Accepted: 25 October 2021 / Published: 27 October 2021
A total of 281 guano samples were collected from caves (N = 181) in eight European countries (Bulgaria, Czech Republic, France, Hungary, Italy, Romania, Slovakia and Slovenia) and attics in the Czech R. (N = 100). The correlation of detection of mycobacteria between Ziehl–Neelsen (ZN) microscopy and culture examination and qPCR was strong. ZN microscopy was positive in guano from caves (58.6%) more than double than positivity in guano from attics (21.0%; p < 0.01). From 89 mycobacterial isolates (73 isolates from cave guano and 16 isolates from attics’ guano), 68 (76.4%) isolates of 19 sp., ssp. and complex were identified as members of three Groups (M. fortuitum, M.chelonae, and M. mucogenicum) and four complexes (M. avium, M. terrae, M.vaccae, and M.smegmatis). A total of 20 isolates (22.5%) belonged to risk group 1 (environmental saprophytes), 48 isolates (53.9%) belonged to risk group 2 (potential pathogens), and none of the isolates belonged to risk group 3 (obligatory pathogens). When comparing bat guano collected from caves and attics, differences (p < 0.01; Mann–Whitney test) were observed for the electrical conductivity, total carbon, total organic, and total inorganic carbon. No difference (p > 0.05; Mann–Whitney test) was found for pH and oxidation-reduction potential parameters. View Full-Text
Keywords: non-tuberculous mycobacteria (NTM); mycobacteria other than tuberculosis (MOTT); saprophytic environmental mycobacteria; risk groups of microorganisms; microchiroptera non-tuberculous mycobacteria (NTM); mycobacteria other than tuberculosis (MOTT); saprophytic environmental mycobacteria; risk groups of microorganisms; microchiroptera
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MDPI and ACS Style

Pavlik, I.; Ulmann, V.; Modra, H.; Gersl, M.; Rantova, B.; Zukal, J.; Zukalova, K.; Konecny, O.; Kana, V.; Kubalek, P.; Babak, V.; Weston, R.T. Nontuberculous Mycobacteria Prevalence in Bats’ Guano from Caves and Attics of Buildings Studied by Culture and qPCR Examinations. Microorganisms 2021, 9, 2236. https://doi.org/10.3390/microorganisms9112236

AMA Style

Pavlik I, Ulmann V, Modra H, Gersl M, Rantova B, Zukal J, Zukalova K, Konecny O, Kana V, Kubalek P, Babak V, Weston RT. Nontuberculous Mycobacteria Prevalence in Bats’ Guano from Caves and Attics of Buildings Studied by Culture and qPCR Examinations. Microorganisms. 2021; 9(11):2236. https://doi.org/10.3390/microorganisms9112236

Chicago/Turabian Style

Pavlik, Ivo, Vit Ulmann, Helena Modra, Milan Gersl, Barbora Rantova, Jan Zukal, Katerina Zukalova, Ondrej Konecny, Vlastislav Kana, Pavel Kubalek, Vladimir Babak, and Ross T. Weston. 2021. "Nontuberculous Mycobacteria Prevalence in Bats’ Guano from Caves and Attics of Buildings Studied by Culture and qPCR Examinations" Microorganisms 9, no. 11: 2236. https://doi.org/10.3390/microorganisms9112236

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