Intraspecies Variation in Tetrahymena rostrata

Watt, Anne; Young, Neil; Haites, Ruth; Dunse, Kerry; Russell, Derek; Billman-Jacobe, Helen

doi:10.3390/microorganisms9102100

Open AccessArticle

Intraspecies Variation in Tetrahymena rostrata

by

Anne Watt

¹,

Neil Young

²

,

Ruth Haites

¹,

Kerry Dunse

²,

Derek Russell

¹ and

Helen Billman-Jacobe

^1,*

¹

Asia-Pacific Centre for Animal Health, Melbourne Veterinary School, Faculty of Veterinary and Agricultural Sciences, University of Melbourne, Parkville, VIC 3010, Australia

²

Melbourne Veterinary School, Faculty of Veterinary and Agricultural Sciences, University of Melbourne, Parkville, VIC 3010, Australia

^*

Author to whom correspondence should be addressed.

Microorganisms 2021, 9(10), 2100; https://doi.org/10.3390/microorganisms9102100

Submission received: 16 July 2021 / Revised: 21 September 2021 / Accepted: 27 September 2021 / Published: 5 October 2021

(This article belongs to the Special Issue Ciliates as Model Organisms: From ‘omics’ to Genetics, Ecology and Signaling)

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Abstract

Two distinct isolates of the facultative parasite, Tetrahymena rostrata were compared, identifying and utilising markers that are useful for studying clonal variation within the species were identified and utilised. The sequences of mitochondrial genomes and several nuclear genes were determined using Illumina short read sequencing. The two T. rostrata isolates had similar morphology. The linear mitogenomes had the gene content and organisation typical of the Tetrahymena genus, comprising 8 tRNA genes, 6 ribosomal RNA genes and 45 protein coding sequences (CDS), twenty-two of which had known function. The two isolates had nucleotide identity within common nuclear markers encoded within the histone H3 and H4 and small subunit ribosomal RNA genes and differed by only 2–4 nucleotides in a region of the characterised actin genes. Variation was observed in several mitochondrial genes and was used to determine intraspecies variation and may reflect the natural history of T. rostrata from different hosts or the geographic origins of the isolates.

Keywords: ciliophora; mtSSUrRNA; histone; cytochrome oxidase 1; origin of replication

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MDPI and ACS Style

Watt, A.; Young, N.; Haites, R.; Dunse, K.; Russell, D.; Billman-Jacobe, H. Intraspecies Variation in Tetrahymena rostrata. Microorganisms 2021, 9, 2100. https://doi.org/10.3390/microorganisms9102100

AMA Style

Watt A, Young N, Haites R, Dunse K, Russell D, Billman-Jacobe H. Intraspecies Variation in Tetrahymena rostrata. Microorganisms. 2021; 9(10):2100. https://doi.org/10.3390/microorganisms9102100

Chicago/Turabian Style

Watt, Anne, Neil Young, Ruth Haites, Kerry Dunse, Derek Russell, and Helen Billman-Jacobe. 2021. "Intraspecies Variation in Tetrahymena rostrata" Microorganisms 9, no. 10: 2100. https://doi.org/10.3390/microorganisms9102100

APA Style

Watt, A., Young, N., Haites, R., Dunse, K., Russell, D., & Billman-Jacobe, H. (2021). Intraspecies Variation in Tetrahymena rostrata. Microorganisms, 9(10), 2100. https://doi.org/10.3390/microorganisms9102100

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

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Intraspecies Variation in Tetrahymena rostrata

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