Intake of Lactobacillus delbrueckii (pExu:hsp65) Prevents the Inflammation and the Disorganization of the Intestinal Mucosa in a Mouse Model of Mucositis

Barroso, Fernanda Alvarenga Lima; de Jesus, Luís Cláudio Lima; de Castro, Camila Prosperi; Batista, Viviane Lima; Ferreira, Ênio; Fernandes, Renata Salgado; de Barros, André Luís Branco; Leclerq, Sophie Yvette; Azevedo, Vasco; Mancha-Agresti, Pamela; Drumond, Mariana Martins

doi:10.3390/microorganisms9010107

Open AccessArticle

Intake of Lactobacillus delbrueckii (pExu:hsp65) Prevents the Inflammation and the Disorganization of the Intestinal Mucosa in a Mouse Model of Mucositis

by

Fernanda Alvarenga Lima Barroso

^1,†,

Luís Cláudio Lima de Jesus

^1,†,

Camila Prosperi de Castro

¹,

Viviane Lima Batista

¹,

Ênio Ferreira

²

,

Renata Salgado Fernandes

³,

André Luís Branco de Barros

³

,

Sophie Yvette Leclerq

⁴,

Vasco Azevedo

¹,

Pamela Mancha-Agresti

^1,5,* and

Mariana Martins Drumond

^1,6,*

¹

Laboratório de Genética Celular e Molecular (LGCM), Departamento de—Genética, Ecologia e Evolução, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte 31270-901, Brazil

²

Departamento de Patologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Brazil

³

Departamento de Análises Clínicas e Toxicológicas, Faculdade de Farmácia, Campus da UFMG, Universidade Federal de Minas Gerais, Cidade Universitária, Belo Horizonte 31270-901, Brazil

⁴

Laboratório de Inovação Biotecnológica, Fundação Ezequiel Dias (FUNED), Belo Horizonte 30510-010, Brazil

⁵

Faculdade de Minas-Faminas-BH, Medicina, Belo Horizonte 31744-007, Brazil

⁶

Centro Federal de Educação Tecnológica de Minas Gerais (CEFET/MG), Departamento de Ciências Biológicas, Belo Horizonte 31421-169, Brazil

^*

Authors to whom correspondence should be addressed.

^†

These authors contributed equally to this work.

Microorganisms 2021, 9(1), 107; https://doi.org/10.3390/microorganisms9010107

Submission received: 2 November 2020 / Revised: 24 November 2020 / Accepted: 30 November 2020 / Published: 5 January 2021

(This article belongs to the Section Microbial Biotechnology)

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Abstract

5-Fluorouracil (5-FU) is an antineoplastic drug that causes, as a side effect, intestinal mucositis, acute inflammation in the small bowel. The Heat Shock Protein (Hsp) are highly expressed in inflammatory conditions, developing an important role in immune modulation. Thus, they are potential candidates for the treatment of inflammatory diseases. In the mucositis mouse model, the present study aimed to evaluate the beneficial effect of oral administration of milk fermented by Lactobacillus delbrueckii CIDCA 133 (pExu:hsp65), a recombinant strain. This approach showed increased levels of sIgA in the intestinal fluid, reducing inflammatory infiltrate and intestinal permeability. Additionally, the histological score was improved. Protection was associated with a reduction in the gene expression of pro-inflammatory cytokines such as Tnf, Il6, Il12, and Il1b, and an increase in Il10, Muc2, and claudin 1 (Cldn1) and 2 (Cldn2) gene expression in ileum tissue. These findings are corroborated with the increased number of goblet cells, the electronic microscopy images, and the reduction of intestinal permeability. The administration of milk fermented by this recombinant probiotic strain was also able to reverse the high levels of gene expression of Tlrs caused by the 5-FU. Thus, the rCIDCA 133:Hsp65 strain was revealed to be a promising preventive strategy for small bowel inflammation.

Keywords: recombinant probiotics; DNA delivery; intestinal mucositis; inflammation; technetium-99m; bacterial translocation; gene expression

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MDPI and ACS Style

Barroso, F.A.L.; de Jesus, L.C.L.; de Castro, C.P.; Batista, V.L.; Ferreira, Ê.; Fernandes, R.S.; de Barros, A.L.B.; Leclerq, S.Y.; Azevedo, V.; Mancha-Agresti, P.; et al. Intake of Lactobacillus delbrueckii (pExu:hsp65) Prevents the Inflammation and the Disorganization of the Intestinal Mucosa in a Mouse Model of Mucositis. Microorganisms 2021, 9, 107. https://doi.org/10.3390/microorganisms9010107

AMA Style

Barroso FAL, de Jesus LCL, de Castro CP, Batista VL, Ferreira Ê, Fernandes RS, de Barros ALB, Leclerq SY, Azevedo V, Mancha-Agresti P, et al. Intake of Lactobacillus delbrueckii (pExu:hsp65) Prevents the Inflammation and the Disorganization of the Intestinal Mucosa in a Mouse Model of Mucositis. Microorganisms. 2021; 9(1):107. https://doi.org/10.3390/microorganisms9010107

Chicago/Turabian Style

Barroso, Fernanda Alvarenga Lima, Luís Cláudio Lima de Jesus, Camila Prosperi de Castro, Viviane Lima Batista, Ênio Ferreira, Renata Salgado Fernandes, André Luís Branco de Barros, Sophie Yvette Leclerq, Vasco Azevedo, Pamela Mancha-Agresti, and et al. 2021. "Intake of Lactobacillus delbrueckii (pExu:hsp65) Prevents the Inflammation and the Disorganization of the Intestinal Mucosa in a Mouse Model of Mucositis" Microorganisms 9, no. 1: 107. https://doi.org/10.3390/microorganisms9010107

APA Style

Barroso, F. A. L., de Jesus, L. C. L., de Castro, C. P., Batista, V. L., Ferreira, Ê., Fernandes, R. S., de Barros, A. L. B., Leclerq, S. Y., Azevedo, V., Mancha-Agresti, P., & Drumond, M. M. (2021). Intake of Lactobacillus delbrueckii (pExu:hsp65) Prevents the Inflammation and the Disorganization of the Intestinal Mucosa in a Mouse Model of Mucositis. Microorganisms, 9(1), 107. https://doi.org/10.3390/microorganisms9010107

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Intake of Lactobacillus delbrueckii (pExu:hsp65) Prevents the Inflammation and the Disorganization of the Intestinal Mucosa in a Mouse Model of Mucositis

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