Regulation of Chitin-Dependent Growth and Natural Competence in Vibrio parahaemolyticus

Vibrios can degrade chitin surfaces to soluble N-acetyl glucosamine oligosaccharides (GlcNAc_n) that can be utilized as a carbon source and also induce a state of natural genetic competence. In this study, we characterized chitin-dependent growth and natural competence in Vibrio parahaemolyticus and its regulation. We found that growth on chitin was regulated through chitin sensors ChiS (sensor histidine kinase) and TfoS (transmembrane transcriptional regulator) by predominantly controlling the expression of chitinase VPA0055 (ChiA2) in a TfoX-dependent manner. The reduced growth of ΔchiA2, ΔchiS and ΔtfoS mutants highlighted the critical role played by ChiA2 in chitin breakdown. This growth defect of ΔchiA2 mutant could be recovered when chitin oligosaccharides GlcNAc₂ or GlcNAc₆ were supplied instead of chitin. The ΔtfoS mutant was also able to grow on GlcNAc₂ but the ΔchiS mutant could not, which indicates that GlcNAc₂ catabolic operon is dependent on ChiS and independent of TfoS. However, the ΔtfoS mutant was unable to utilize GlcNAc₆ because the periplasmic enzymes required for the breakdown of GlcNAc₆ were found to be downregulated at the mRNA level. We also showed that natural competence can be induced only by GlcNAc₆, not GlcNAc₂, because the expression of competence genes was significantly higher in the presence of GlcNAc₆ compared to GlcNAc₂. Moreover, this might be an indication that GlcNAc₂ and GlcNAc₆ were detected by different receptors. Therefore, we speculate that GlcNAc₂-dependent activation of ChiS and GlcNAc₆-dependent activation of TfoS might be crucial for the induction of natural competence in V. parahaemolyticus through the upregulation of the master competence regulator TfoX.