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Crucial Role of the C-Terminal Domain of Hfq Protein in Genomic Instability

Laboratory of DNA Structure and Mutagenesis, Department of Biology, Chemistry and Health Sciences, South Dakota School of Mines and Technology, Rapid City, SD 57701, USA
Synchrotron SOLEIL, 91192 Gif-sur-Yvette, France
Institut de Physique et Chimie des Matériaux de Strasbourg (IPCMS), Département d’Optique ultrarapide et de Nanophotonique (DON), 23, rue du Loess, BP 43, CEDEX 2, 67034 Strasbourg, France
UFR Sciences du vivant–Université de Paris, F-75006 Paris, France
Laboratoire Léon Brillouin (LLB), CEA, CNRS UMR12, Université Paris Saclay, CEA Saclay, 91191 Gif-sur-Yvette, France
Authors to whom correspondence should be addressed.
Microorganisms 2020, 8(10), 1598;
Received: 28 July 2020 / Revised: 10 October 2020 / Accepted: 13 October 2020 / Published: 17 October 2020
G-rich DNA repeats that can form G-quadruplex structures are prevalent in bacterial genomes and are frequently associated with regulatory regions of genes involved in virulence, antigenic variation, and antibiotic resistance. These sequences are also inherently mutagenic and can lead to changes affecting cell survival and adaptation. Transcription of the G-quadruplex-forming repeat (G3T)n in E. coli, when mRNA comprised the G-rich strand, promotes G-quadruplex formation in DNA and increases rates of deletion of G-quadruplex-forming sequences. The genomic instability of G-quadruplex repeats may be a source of genetic variability that can influence alterations and evolution of bacteria. The DNA chaperone Hfq is involved in the genetic instability of these G-quadruplex sequences. Inactivation of the hfq gene decreases the genetic instability of G-quadruplex, demonstrating that the genomic instability of this regulatory element can be influenced by the E. coli highly pleiotropic Hfq protein, which is involved in small noncoding RNA regulation pathways, and DNA organization and packaging. We have shown previously that the protein binds to and stabilizes these sequences, increasing rates of their genomic instability. Here, we extend this analysis to characterize the role of the C-terminal domain of Hfq protein in interaction with G-quadruplex structures. This allows to better understand the function of this specific region of the Hfq protein in genomic instability. View Full-Text
Keywords: genomic instability; quadruplex; DNA-directed mutagenesis; nucleoid; bacterial chromatin genomic instability; quadruplex; DNA-directed mutagenesis; nucleoid; bacterial chromatin
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MDPI and ACS Style

Parekh, V.J.; Wien, F.; Grange, W.; De Long, T.A.; Arluison, V.; Sinden, R.R. Crucial Role of the C-Terminal Domain of Hfq Protein in Genomic Instability. Microorganisms 2020, 8, 1598.

AMA Style

Parekh VJ, Wien F, Grange W, De Long TA, Arluison V, Sinden RR. Crucial Role of the C-Terminal Domain of Hfq Protein in Genomic Instability. Microorganisms. 2020; 8(10):1598.

Chicago/Turabian Style

Parekh, Virali J., Frank Wien, Wilfried Grange, Thomas A. De Long, Véronique Arluison, and Richard R. Sinden 2020. "Crucial Role of the C-Terminal Domain of Hfq Protein in Genomic Instability" Microorganisms 8, no. 10: 1598.

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