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Article

A Powerful LAMP Weapon against the Threat of the Quarantine Plant Pathogen Curtobacterium flaccumfaciens pv. flaccumfaciens

1
Laboratorio di Patologia Vegetale Molecolare, Dipartimento di Scienze e Tecnologie Agrarie, Alimentari Ambientali e Forestali (DAGRI), Università degli Studi di Firenze, Via della Lastruccia 10, 50019 Sesto Fiorentino (Firenze), Italy
2
Agrarian and Technological Institute (ATI), Peoples’ Friendship University of Russia, Miklukho-Maklaya str.8, 117198 Moscow, Russia
3
Department of Plant Protection, University of Tehran, Karaj 31587-77871, Iran
*
Author to whom correspondence should be addressed.
Microorganisms 2020, 8(11), 1705; https://doi.org/10.3390/microorganisms8111705
Received: 31 August 2020 / Revised: 23 October 2020 / Accepted: 28 October 2020 / Published: 31 October 2020
Curtobacterium flaccumfaciens pv. flaccumfaciens (Cff) is a Gram-positive phytopathogenic bacterium attacking leguminous crops and causing systemic diseases such as the bacterial wilt of beans and bacterial spot of soybeans. Since the early 20th century, Cff is reported to be present in North America, where it still causes high economic losses. Currently, Cff is an emerging plant pathogen, rapidly spreading worldwide and occurring in many bean-producing countries. Infected seeds are the main dissemination pathway for Cff, both over short and long distances. Cff remains viable in the seeds for long times, even in field conditions. According to the most recent EU legislation, Cff is included among the quarantine pests not known to occur in the Union territory, and for which the phytosanitary inspection consists mainly of the visual examination of imported bean seeds. The seedborne nature of Cff combined with the globalization of trades urgently call for the implementation of a highly specific diagnostic test for Cff, to be routinely and easily used at the official ports of entry and into the fields. This paper reports the development of a LAMP (Loop-Mediated Isothermal Amplification) specific for Cff, that allows the detection of Cff in infected seeds, both by fluorescence and visual monitoring, after 30 min of reaction and with a detection limit at around 4 fg/μL of pure Cff genomic DNA. View Full-Text
Keywords: Loop-Mediated Isothermal Amplification; LAMP; Curtobacterium flaccumfaciens pv. flaccumfaciens; bacterial wilt of bean; tan spot of soybean; quarantine plant pathogen; molecular diagnostics Loop-Mediated Isothermal Amplification; LAMP; Curtobacterium flaccumfaciens pv. flaccumfaciens; bacterial wilt of bean; tan spot of soybean; quarantine plant pathogen; molecular diagnostics
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MDPI and ACS Style

Tegli, S.; Biancalani, C.; Ignatov, A.N.; Osdaghi, E. A Powerful LAMP Weapon against the Threat of the Quarantine Plant Pathogen Curtobacterium flaccumfaciens pv. flaccumfaciens. Microorganisms 2020, 8, 1705. https://doi.org/10.3390/microorganisms8111705

AMA Style

Tegli S, Biancalani C, Ignatov AN, Osdaghi E. A Powerful LAMP Weapon against the Threat of the Quarantine Plant Pathogen Curtobacterium flaccumfaciens pv. flaccumfaciens. Microorganisms. 2020; 8(11):1705. https://doi.org/10.3390/microorganisms8111705

Chicago/Turabian Style

Tegli, Stefania, Carola Biancalani, Aleksandr N. Ignatov, and Ebrahim Osdaghi. 2020. "A Powerful LAMP Weapon against the Threat of the Quarantine Plant Pathogen Curtobacterium flaccumfaciens pv. flaccumfaciens" Microorganisms 8, no. 11: 1705. https://doi.org/10.3390/microorganisms8111705

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