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Correction published on 10 September 2020, see Microorganisms 2020, 8(9), 1384.
Open AccessArticle

Characterization of ESBL-Producing Enterobacteria from Fruit Bats in an Unprotected Area of Makokou, Gabon

1
Laboratoire de Microbiologie, Institut de Recherche en Ecologie Tropicale (IRET), (Centre National de recherche Scientifique et Technologique/CENAREST), Libreville B.P. 13354, Gabon
2
Laboratoire de Bactériologie de Recherche, Unité de recherche et d’Analyses Médicales (URAM), Centre Interdisciplinaire de Recherches Médicales de Franceville (CIRMF), Franceville B.P. 679, Gabon
3
UMR CNRS/uB 6282 Biogéosciences, Université de Bourgogne, 6 bd Gabriel, 21000 Dijon, France
4
Département de Chimie, Faculté des Sciences, Université des Sciences et Technique de Masuku (USTM), Franceville B.P. 943, Gabon
5
Laboratoire de Bactériologie, Centre Hospitalier Universitaire de Montpellier, Université de Montpellier, 34 295 Montpellier, France
6
UMR MIVEGEC IRD-CNRS-Université de Montpellier, IRD, 911 Avenue Agropolis, 34394 Montpellier, France
7
Laboratoire d’Ecologie Microbienne, FSNV, Université de Bejaia, Bejaia 06000, Algerie
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Microorganisms 2020, 8(1), 138; https://doi.org/10.3390/microorganisms8010138
Received: 3 December 2019 / Revised: 9 January 2020 / Accepted: 11 January 2020 / Published: 19 January 2020
(This article belongs to the Special Issue Wildlife Microbiology 2.0)
In Gabon, terrestrial mammals of protected areas have been identified as a possible source of antibiotic-resistant bacteria. Some studies on antibiotic resistance in bats have already been carried out. The main goal of our study was to detect extended-spectrum beta-lactamases (ESBLs) that are produced by enterobacteria from bats in the Makokou region in Gabon. Sixty-eight fecal samples were obtained from 68 bats caught in the forests located 1 km from the little town of Makokou. After culture and isolation, 66 Gram-negative bacterial colonies were obtained. The double-disk diffusion test confirmed the presence of ESBLs in six (20.69%) Escherichia coli isolates, four (13.79%) Klebsiella pneumoniae isolates, and one (3.45%) Enterobacter cloacae isolate. The analysis based on the nucleotide sequences of the ESBL resistance genes showed that all cefotaximase-Munichs (CTX-Ms) were CTX-M-15 and that all sulfhydryl variables (SHVs) were SHV-11: 41.67% CTX-M-15-producing E. coli, 16.67% CTX-M-15+SHV-11-producing E. coli, 8.33% CTX-M-15-producing K. pneumoniae, 25% CTX-M-15+SHV-11-producing K. pneumoniae, and 8.33% CTX-M-15-produced E. cloacae. This study shows for the first time the presence of multiresistant ESBL-producing enterobacteria in fruit bats in Makokou. View Full-Text
Keywords: bats; Gram-negative bacteria; ESBL; multiresistance; reservoir bats; Gram-negative bacteria; ESBL; multiresistance; reservoir
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MDPI and ACS Style

Mbehang Nguema, P.P.; Onanga, R.; Ndong Atome, G.R.; Obague Mbeang, J.C.; Mabika Mabika, A.; Yaro, M.; Lounnas, M.; Dumont, Y.; Zohra, Z.F.; Godreuil, S.; Bretagnolle, F. Characterization of ESBL-Producing Enterobacteria from Fruit Bats in an Unprotected Area of Makokou, Gabon. Microorganisms 2020, 8, 138. https://doi.org/10.3390/microorganisms8010138

AMA Style

Mbehang Nguema PP, Onanga R, Ndong Atome GR, Obague Mbeang JC, Mabika Mabika A, Yaro M, Lounnas M, Dumont Y, Zohra ZF, Godreuil S, Bretagnolle F. Characterization of ESBL-Producing Enterobacteria from Fruit Bats in an Unprotected Area of Makokou, Gabon. Microorganisms. 2020; 8(1):138. https://doi.org/10.3390/microorganisms8010138

Chicago/Turabian Style

Mbehang Nguema, Pierre P.; Onanga, Richard; Ndong Atome, Guy R.; Obague Mbeang, Jean C.; Mabika Mabika, Arsène; Yaro, Moussa; Lounnas, Manon; Dumont, Yann; Zohra, Zaidi F.; Godreuil, Sylvain; Bretagnolle, François. 2020. "Characterization of ESBL-Producing Enterobacteria from Fruit Bats in an Unprotected Area of Makokou, Gabon" Microorganisms 8, no. 1: 138. https://doi.org/10.3390/microorganisms8010138

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