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Open AccessArticle

Inactivation of Escherichia coli O157:H7 by High Hydrostatic Pressure Combined with Gas Packaging

by Bing Zhou 1,2,3,4, Luyao Zhang 1,2,3,4, Xiao Wang 1,2,3,4, Peng Dong 1,2,3,4, Xiaosong Hu 1,2,3,4 and Yan Zhang 1,2,3,4,*
1
College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China
2
National Engineering Research Center for Fruits and Vegetables Processing, Ministry of Science and Technology, Beijing 100083, China
3
Key Laboratory of Fruits and Vegetables Processing, Ministry of Agriculture and Rural Affairs, Beijing 100083, China
4
Beijing Key Laboratory of Food Non-Thermal Processing, Beijing 100083, China
*
Author to whom correspondence should be addressed.
Microorganisms 2019, 7(6), 154; https://doi.org/10.3390/microorganisms7060154
Received: 19 April 2019 / Revised: 15 May 2019 / Accepted: 16 May 2019 / Published: 28 May 2019
The inactivation of Escherichia coli O157:H7 (E. coli) in physiological saline and lotus roots by high hydrostatic pressure (HHP) in combination with CO2 or N2 was studied. Changes in the morphology, cellular structure, and membrane permeability of the cells in physiological saline after treatments were investigated using scanning electron microscopy, transmission electron microscopy, and flow cytometry, respectively. It was shown that after HHP treatments at 150–550 MPa, CO2-packed E. coli cells had higher inactivation than the N2-packed and vacuum-packed cells, and no significant difference was observed in the latter two groups. Further, both the morphology and intracellular structure of CO2-packed E.coli cells were strongly destroyed by high hydrostatic pressure. However, serious damage to the intracellular structures occurred in only the N2-packed E. coli cells. During HHP treatments, the presence of CO2 caused more disruptions in the membrane of E. coli cells than in the N2-packed and vacuum-packed cells. These results indicate that the combined treatment of HHP and CO2 had a strong synergistic bactericidal effect, whereas N2 did not have synergistic effects with HHP. Although these two combined treatments had different effects on the inactivation of E. coli cells, the inactivation mechanisms might be similar. During both treatments, E. coli cells were inactivated by cell damage induced to the cellular structure through the membrane components and the extracellular morphology, unlike the independent HHP treatment. View Full-Text
Keywords: high hydrostatic pressure; carbon dioxide; nitrogen; modified atmosphere packaging; Escherichia coli high hydrostatic pressure; carbon dioxide; nitrogen; modified atmosphere packaging; Escherichia coli
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Zhou, B.; Zhang, L.; Wang, X.; Dong, P.; Hu, X.; Zhang, Y. Inactivation of Escherichia coli O157:H7 by High Hydrostatic Pressure Combined with Gas Packaging. Microorganisms 2019, 7, 154.

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