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The Role of Pseudomonas in Heterotrophic Nitrification: A Case Study on Shrimp Ponds (Litopenaeus vannamei) in Soc Trang Province

1
Centre for Environmental Sustainability and Remediation, School of Science, Royal Melbourne Institute of Technology University, PO Box 71, Bundoora Victoria 3083, Australia
2
National Key Laboratory of Gene Technology, Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Hanoi 100000, Vietnam
3
Faculty of Biotechnology, Graduate University of Science and Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Hanoi 100000, Vietnam
4
Department of Aquatic Pathology, College of Aquaculture and Fisheries, Can Tho University, Can Tho 900000, Vietnam
*
Author to whom correspondence should be addressed.
Microorganisms 2019, 7(6), 155; https://doi.org/10.3390/microorganisms7060155
Received: 2 May 2019 / Revised: 22 May 2019 / Accepted: 28 May 2019 / Published: 29 May 2019
(This article belongs to the Special Issue Microbiological Wastewater Treatment)
Based on a total of 6,295,650 sequences from the V3 and V4 regions (16S ribosomal RNA), the composition of the microorganism communities in the water of three Litopenaeus vannamei (Decapoda, Whiteleg shrimp; Soc Trang, Vietnam) ponds were identified. Pseudomonas (10–20.29%), Methylophilus (13.26–24.28%), and Flavobacterium (2.6–19.29%) were the most abundant genera. The total ammonia (TAN) concentration (p = 0.025) and temperature (p = 0.015) were significantly correlated with the relative abundance of Pseudomonas in two bacterial communities (ST1, ST4), whereas the predictive functions of microorganism communities based on 16S rRNA gene data was estimated using Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUST), which showed that nitrogen metabolism was significantly negatively correlated (p = 0.049) with TAN concentration. The abundance of Pseudomonas and nitrogen metabolism increased with a decrease in TAN concentration. The correlation between TAN concentration and the abundance of Pseudomonas was followed by the isolation, and heterotrophic nitrifying performance analysis was used to confirm our findings. Six Pseudomonas strains capable of heterotrophic nitrification were isolated from the three water samples and showed a complete reduction of 100 mg/L NH4Cl during a 96-h cultivation. These results indicate the potential of applying Pseudomonas in shrimp ponds for water treatment. View Full-Text
Keywords: Pseudomonas; Litopenaeus vannamei; heterotrophic nitrification; ammonia Pseudomonas; Litopenaeus vannamei; heterotrophic nitrification; ammonia
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Trung Tran, T.; Bott, N.J.; Dai Lam, N.; Trung Nguyen, N.; Hoang Thi Dang, O.; Hoang Le, D.; Tung Le, L.; Hoang Chu, H. The Role of Pseudomonas in Heterotrophic Nitrification: A Case Study on Shrimp Ponds (Litopenaeus vannamei) in Soc Trang Province. Microorganisms 2019, 7, 155.

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