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Transcriptional Expression of the ompA, cpaf, tarp, and tox Genes of Chlamydia trachomatis Clinical Isolates at Different Stages of the Developmental Cycle

1
Department of Virology, University of Helsinki and Helsinki University Hospital, FI-00014 Helsinki, Finland
2
Department of Obstetrics and Gynecology, University of Helsinki and Helsinki University Hospital, FI-00029 HUS Helsinki, Finland
3
Clinic of Venereal Diseases, Skin and Allergy Hospital, Helsinki University Hospital, FI-00029 HUS Helsinki, Finland
*
Author to whom correspondence should be addressed.
Microorganisms 2019, 7(6), 153; https://doi.org/10.3390/microorganisms7060153
Received: 1 May 2019 / Revised: 24 May 2019 / Accepted: 25 May 2019 / Published: 28 May 2019
(This article belongs to the Special Issue Chlamydiae and Chlamydia like Bacteria)
The transcriptional gene expression patterns of Chlamydia trachomatis have mainly been studied using reference strains propagated in cultured cells. Here, using five low-passage-number C. trachomatis clinical isolates that originated from asymptomatic or symptomatic female patients, the in vitro expression of the ompA, cpaf, tarp, and tox genes was studied with reverse transcriptase real-time PCR during the chlamydial developmental cycle. We observed dissimilarities in the gene expression patterns between the low-passage-number clinical isolates and the reference strains. The expression of ompA and the peak of the tox expression were observed earlier in the reference strains than in most of the clinical isolates. The expression of cpaf was high in the reference strains compared with the clinical isolates at the mid-phase (6–24 hours post infection) of the developmental cycle. All of the strains had a rather similar tarp expression profile. Four out of five clinical isolates exhibited slower growth kinetics compared with the reference strains. The use of low-passage-number C. trachomatis clinical isolates instead of reference strains in the studies might better reflect the situation in human infection. View Full-Text
Keywords: Chlamydia trachomatis; transcriptional expression; low-passage-number isolates Chlamydia trachomatis; transcriptional expression; low-passage-number isolates
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Korhonen, S.; Hokynar, K.; Mannonen, L.; Paavonen, J.; Hiltunen-Back, E.; Puolakkainen, M. Transcriptional Expression of the ompA, cpaf, tarp, and tox Genes of Chlamydia trachomatis Clinical Isolates at Different Stages of the Developmental Cycle. Microorganisms 2019, 7, 153.

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