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Volume 7
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10.3390/microorganisms7120703
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Article

Chlamydia trachomatis Whole-Proteome Microarray Analysis of The Netherlands Chlamydia Cohort Study

by
Katrin Hufnagel
1,*,†,
Bernice Hoenderboom
2,3,*,†,
Christoph Harmel
1,
Juliane K. Rohland
1,
Birgit H.B. van Benthem
2,
Servaas A. Morré
3,4 and
Tim Waterboer
1
1
Infections and Cancer Epidemiology, German Cancer Research Center (Deutsches Krebsforschungszentrum, DKFZ), 69120 Heidelberg, Germany
2
Epidemiology and Surveillance Unit, Centre for Infectious Disease Control, National Institute for Public Health and the Environment, 3721MA Bilthoven, The Netherlands
3
Laboratory of Immunogenetics, Department Medical Microbiology and Infection Control, location VU University Medical Center, Amsterdam University Medical Centre (UMC), De Boelelaan 1108, 1081 HZ Amsterdam, The Netherlands
4
Institute for Public Health Genomics (IPHG), Department of Genetics and Cell Biology, Research School GROW (School for Oncology & Developmental Biology), Faculty of Health, Medicine & Life Sciences, University of Maastricht, 6229 ER Maastricht, The Netherlands
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Microorganisms 2019, 7(12), 703; https://doi.org/10.3390/microorganisms7120703
Submission received: 11 November 2019 / Revised: 10 December 2019 / Accepted: 12 December 2019 / Published: 16 December 2019
(This article belongs to the Special Issue Chlamydiae and Chlamydia like Bacteria)
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Abstract

Chlamydia trachomatis (Ct) whole-proteome microarrays were utilized to identify antibody patterns associated with infection; pelvic inflammatory disease (PID), tubal factor infertility, chronic pelvic pain (CPP) and ectopic pregnancy in a subsample of the Netherlands Chlamydia cohort study. Serum pools were analyzed on whole-proteome arrays. The 121 most reactive antigens identified during whole-proteome arrays were selected for further analysis with minimized microarrays that allowed for single sera analysis. From the 232 single sera; 145 (62.5%) serum samples were reactive for at least one antigen. To discriminate between positive and negative serum samples; we created a panel of in total 18 antigens which identified 96% of all microarray positive samples. Antigens CT_858; CT_813 and CT_142 were most reactive. Comparison of antibody reactivity’s among women with and without Ct related sequelae revealed that the reactivity of CT_813 and CT_142 was less common among women with PID compared to women without (29.0% versus 58.6%, p = 0.005 and 25.8% versus 50.6%, p = 0.017 respectively). CT_858 was less common among CPP cases compared to controls (33.3% versus 58.6; p = 0.028). Using a whole-proteome array to select antigens for minimized arrays allows for the identification of novel informative antigens as general infection markers or disease associated antigens
Keywords: Chlamydia trachomatis; whole-proteome microarrays; serology; antigen identification Chlamydia trachomatis; whole-proteome microarrays; serology; antigen identification

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MDPI and ACS Style

Hufnagel, K.; Hoenderboom, B.; Harmel, C.; Rohland, J.K.; van Benthem, B.H.B.; Morré, S.A.; Waterboer, T. Chlamydia trachomatis Whole-Proteome Microarray Analysis of The Netherlands Chlamydia Cohort Study. Microorganisms 2019, 7, 703. https://doi.org/10.3390/microorganisms7120703

AMA Style

Hufnagel K, Hoenderboom B, Harmel C, Rohland JK, van Benthem BHB, Morré SA, Waterboer T. Chlamydia trachomatis Whole-Proteome Microarray Analysis of The Netherlands Chlamydia Cohort Study. Microorganisms. 2019; 7(12):703. https://doi.org/10.3390/microorganisms7120703

Chicago/Turabian Style

Hufnagel, Katrin, Bernice Hoenderboom, Christoph Harmel, Juliane K. Rohland, Birgit H.B. van Benthem, Servaas A. Morré, and Tim Waterboer. 2019. "Chlamydia trachomatis Whole-Proteome Microarray Analysis of The Netherlands Chlamydia Cohort Study" Microorganisms 7, no. 12: 703. https://doi.org/10.3390/microorganisms7120703

APA Style

Hufnagel, K., Hoenderboom, B., Harmel, C., Rohland, J. K., van Benthem, B. H. B., Morré, S. A., & Waterboer, T. (2019). Chlamydia trachomatis Whole-Proteome Microarray Analysis of The Netherlands Chlamydia Cohort Study. Microorganisms, 7(12), 703. https://doi.org/10.3390/microorganisms7120703

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