Dietary Saccharomyces cerevisiae boulardii CNCM I-1079 Positively Affects Performance and Intestinal Ecosystem in Broilers during a Campylobacter jejuni Infection
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animal Experimental Design
2.2. Feed Analyses
2.3. Experimental Challenge
2.4. Microbiological Analyses
2.5. Histo-Morphometrical Analyses
2.6. Gut Microbiota Sampling and DNA Analysis
2.7. Bioinformatic Analyses
2.8. Biostatistical Analyses
3. Results
3.1. Animal Performances
3.2. Microbiological Analyses
3.3. Histo-Morphometrical Analyses
3.4. Gut Microbiota Analyses
3.4.1. Bacterial Phylogenetic Composition and Cecal Core Microbiota in Broilers
3.4.2. Time Dependency of Fecal Microbiota Composition and Diversity
3.4.3. Effects of Supplementation in Fecal Microbiota Composition and Diversity
3.4.4. Time Dependency of Cecal Microbiota Composition and Diversity
3.4.5. Abundances of OTUs Related to F. prausnitzii in Fecal and Cecal Contents According to Groups
3.4.6. Supplementation-Related Effects on Abundances of OTUs Related to Campylobacter spp. in Fecal and Cecal Contents
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Items | Diet x Time | Effects | p | ||||
Live weight | Day 1 | Day 21 | Day 28 | Day 40 | |||
C | 40.74 G | 464.45 E | 824.23 C | 1791.46 B | Diet | <0.0001 | |
S | 36.14 G | 667.11 F | 1078.67 D | 2021.03 A | Time | <0.0001 | |
Diet × time | <0.0001 | ||||||
SEM | 17.980 | 17.980 | 17.980 | 24.24 | |||
ADG | Day 0–21 | Day 21–27 | Day 28–40 | Overall | |||
C | 21.18 D | 59.96 B | 74.66 A | 51.94 | Diet | 0.0008 | |
S | 31.53 C | 68.75 A | 72.84 A | 57.71 | Time | <0.0001 | |
Diet × time | 0.0062 | ||||||
SEM | 1.901 | 1.098 |
FCR | Day 0–21 | Day 22–27 | Day 28–40 |
---|---|---|---|
C | 1.87 A | 1.95 A | 2.08 |
S | 1.72 B | 1.85 B | 1.92 |
SEM | 0.046 | 0.050 | 0.061 |
p | 0.0423 | 0.024 | 0.0837 |
Group | Morphometrical Analyses | PAS positive cells | |||
---|---|---|---|---|---|
Villi length (µ) | Crypt depth (µ) | V/C ratio | Villi | Crypt | |
C | 2688.92 B | 352.66 B | 7.965 | 136.99 | 19.48 |
S | 3870.82 A | 428.78 A | 9.714 | 188.18 | 20.76 |
SEM | 111.844 | 20.504 | 0.540 | 27.748 | 0.808 |
p | <0.0001 | 0.0333 | 0.0617 | 0.1943 | 0.265 |
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Massacci, F.R.; Lovito, C.; Tofani, S.; Tentellini, M.; Genovese, D.A.; De Leo, A.A.P.; Papa, P.; Magistrali, C.F.; Manuali, E.; Trabalza-Marinucci, M.; et al. Dietary Saccharomyces cerevisiae boulardii CNCM I-1079 Positively Affects Performance and Intestinal Ecosystem in Broilers during a Campylobacter jejuni Infection. Microorganisms 2019, 7, 596. https://doi.org/10.3390/microorganisms7120596
Massacci FR, Lovito C, Tofani S, Tentellini M, Genovese DA, De Leo AAP, Papa P, Magistrali CF, Manuali E, Trabalza-Marinucci M, et al. Dietary Saccharomyces cerevisiae boulardii CNCM I-1079 Positively Affects Performance and Intestinal Ecosystem in Broilers during a Campylobacter jejuni Infection. Microorganisms. 2019; 7(12):596. https://doi.org/10.3390/microorganisms7120596
Chicago/Turabian StyleMassacci, Francesca Romana, Carmela Lovito, Silvia Tofani, Michele Tentellini, Domenica Anna Genovese, Alessia Arcangela Pia De Leo, Paola Papa, Chiara Francesca Magistrali, Elisabetta Manuali, Massimo Trabalza-Marinucci, and et al. 2019. "Dietary Saccharomyces cerevisiae boulardii CNCM I-1079 Positively Affects Performance and Intestinal Ecosystem in Broilers during a Campylobacter jejuni Infection" Microorganisms 7, no. 12: 596. https://doi.org/10.3390/microorganisms7120596