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Open AccessArticle

Bioremediation of Artificial Diesel-Contaminated Soil Using Bacterial Consortium Immobilized to Plasma-Pretreated Wood Waste

1
Department of Chemical Engineering and Biotechnology, Ariel University, Ariel 40700, Israel
2
Dead Sea-Arava Science Center, Arava 86910, Israel
*
Author to whom correspondence should be addressed.
Microorganisms 2019, 7(11), 497; https://doi.org/10.3390/microorganisms7110497
Received: 21 August 2019 / Revised: 19 September 2019 / Accepted: 26 October 2019 / Published: 28 October 2019
(This article belongs to the Special Issue Microbial Degradation of Xenobiotics)
Bioaugmentation is a bioremediation option based on increasing the natural in-situ microbial population that possesses the ability to degrade the contaminating pollutant. In this study, a diesel-degrading consortium was obtained from an oil-contaminated soil. The diesel-degrading consortium was grown on wood waste that was plasma-pretreated. This plasma treatment led to an increase of bacterial attachment and diesel degradation rates. On the 7th day the biofilm viability on the plasma-treated wood waste reached 0.53 ± 0.02 OD 540 nm, compared to the non-treated wood waste which was only 0.34 ± 0.02. Biofilm attached to plasma-treated and untreated wood waste which was inoculated into artificially diesel-contaminated soil (0.15% g/g) achieved a degradation rate of 9.3 mg day−1 and 7.8 mg day−1, respectively. While, in the soil that was inoculated with planktonic bacteria, degradation was only 5.7 mg day−1. Exposing the soil sample to high temperature (50 °C) or to different soil acidity did not influence the degradation rate of the biofilm attached to the plasma-treated wood waste. The two most abundant bacterial distributions at the family level were Xanthomonadaceae and Sphingomonadaceae. To our knowledge, this is the first study that showed the advantages of biofilm attached to plasma-pretreated wood waste for diesel biodegradation in soil. View Full-Text
Keywords: bioremediation; bioaugmentation; biofilm; diesel; contaminated soil bioremediation; bioaugmentation; biofilm; diesel; contaminated soil
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Farber, R.; Rosenberg, A.; Rozenfeld, S.; Benet, G.; Cahan, R. Bioremediation of Artificial Diesel-Contaminated Soil Using Bacterial Consortium Immobilized to Plasma-Pretreated Wood Waste. Microorganisms 2019, 7, 497.

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