This is an early access version, the complete PDF, HTML, and XML versions will be available soon.
Open AccessArticle
Role of Saccharomyces cerevisiae Fcy Proteins and Their Homologs in the Catabolism of Modified Heterocyclic Pyrimidine Bases
1
Department of Chemistry and Bioengineering, Faculty of Fundamental Sciences, Vilnius Gediminas Technical University (VILNIUS TECH), Saulėtekio al. 11, 10223 Vilnius, Lithuania
2
Laboratory of Genetics, State Scientific Research Institute Nature Research Centre, Akademijos str. 2, 08412 Vilnius, Lithuania
*
Author to whom correspondence should be addressed.
Microorganisms 2025, 13(7), 1506; https://doi.org/10.3390/microorganisms13071506 (registering DOI)
Submission received: 23 May 2025
/
Revised: 25 June 2025
/
Accepted: 26 June 2025
/
Published: 27 June 2025
Abstract
The synthesis of various heterocyclic base modifications of nucleic acids has been thoroughly investigated; however, much less is known about their catabolism. Also, little is known about the transport of such compounds across the microbial cell membranes. Using the Saccharomyces cerevisiae single-gene deletion library, we performed genome-wide screening for genes affecting the growth of yeast in minimal media supplemented with N4-acetylcytosine as a source of uracil. We found that Fcy1, Fcy21, Bud16, Gnd1, and Fur4 proteins are required for efficient growth in the tested medium. Additionally, we used several heterocyclic pyrimidine bases and Fcy homolog mutants to test their growth in respective minimal media. We found that tested permeases differently affect the growth of yeast that is dependent on the heterocyclic pyrimidine bases used as a source of uracil. The most pronounced effect was observed for the ∆fur4 mutant, which was growing much slower than the corresponding wild-type strain in the media supplemented with N4-acetylcytosine, 4-methylthiouracil, N4-methylcytosine, N4,N4-dimethylcytosine, 2-thiouracil, or 4-thiouracil. We suggest that Fur4 protein is the major yeast transporter of modified heterocyclic pyrimidine bases. Our observations might be helpful when investigating the actions of various heterocyclic base-based antifungal, anticancer, and antiviral drugs.
Share and Cite
MDPI and ACS Style
Urbonavičius, J.; Vepštaitė-Monstavičė, I.; Lukša-Žebelovič, J.; Servienė, E.; Tauraitė, D.
Role of Saccharomyces cerevisiae Fcy Proteins and Their Homologs in the Catabolism of Modified Heterocyclic Pyrimidine Bases. Microorganisms 2025, 13, 1506.
https://doi.org/10.3390/microorganisms13071506
AMA Style
Urbonavičius J, Vepštaitė-Monstavičė I, Lukša-Žebelovič J, Servienė E, Tauraitė D.
Role of Saccharomyces cerevisiae Fcy Proteins and Their Homologs in the Catabolism of Modified Heterocyclic Pyrimidine Bases. Microorganisms. 2025; 13(7):1506.
https://doi.org/10.3390/microorganisms13071506
Chicago/Turabian Style
Urbonavičius, Jaunius, Iglė Vepštaitė-Monstavičė, Juliana Lukša-Žebelovič, Elena Servienė, and Daiva Tauraitė.
2025. "Role of Saccharomyces cerevisiae Fcy Proteins and Their Homologs in the Catabolism of Modified Heterocyclic Pyrimidine Bases" Microorganisms 13, no. 7: 1506.
https://doi.org/10.3390/microorganisms13071506
APA Style
Urbonavičius, J., Vepštaitė-Monstavičė, I., Lukša-Žebelovič, J., Servienė, E., & Tauraitė, D.
(2025). Role of Saccharomyces cerevisiae Fcy Proteins and Their Homologs in the Catabolism of Modified Heterocyclic Pyrimidine Bases. Microorganisms, 13(7), 1506.
https://doi.org/10.3390/microorganisms13071506
Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details
here.
Article Metrics
Article Access Statistics
For more information on the journal statistics, click
here.
Multiple requests from the same IP address are counted as one view.
, 