Next Article in Journal
Role of Saccharomyces cerevisiae Fcy Proteins and Their Homologs in the Catabolism of Modified Heterocyclic Pyrimidine Bases
Previous Article in Journal
Comparison of Microbiological Profiles of Primary Hip and Knee Peri-Prosthetic Joint Infections Treated at Specialist Centers Around the World
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Microorganisms
Volume 13
Issue 7
10.3390/microorganisms13071507
microorganisms-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
This is an early access version, the complete PDF, HTML, and XML versions will be available soon.
Article

Probiotic Supplementation Improves Gut Microbiota in Chronic Metabolic and Cardio-Cerebrovascular Diseases Among Chinese Adults over 60: Study Using Cross-Sectional and Longitudinal Cohorts

by
Xi Wang
1,2,
Wanting Dong
1,
Qiuying Liu
1,
Xi Zeng
3,
Yan Liu
4,
Zheng Li
1,
Yuanlong Pan
1,
Qian Xiong
1,
Na Lyu
1,* and
Baoli Zhu
1,2,5,6,7,*
1
CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
2
University of Chinese Academy of Sciences, Beijing 100049, China
3
Beijing Children’s Hospital, Capital Medical University, Beijing 100069, China
4
Yingdong Intelligent Technology (Shandong) Co., Ltd., Jinan 250000, China
5
Department of Pathogenic Biology, School of Basic Medical Sciences, Southwest Medical University, Luzhou 646000, China
6
Jinan Microecological Biomedicine Shandong Laboratory, Jinan 250117, China
7
Beijing Key Laboratory of Antimicrobial Resistance and Pathogen Genomics, Beijing 100101, China
*
Authors to whom correspondence should be addressed.
Microorganisms 2025, 13(7), 1507; https://doi.org/10.3390/microorganisms13071507 (registering DOI)
Submission received: 27 May 2025 / Revised: 22 June 2025 / Accepted: 25 June 2025 / Published: 27 June 2025
(This article belongs to the Section Gut Microbiota)
Review Reports Versions Notes

Abstract

Probiotics demonstrate the ability to maintain intestinal homeostasis and promote gut health. However, their effects on gut microbiota in adults over 60 years old with chronic metabolic disease (CMD) or cardio-cerebrovascular disease (CCD) remain poorly understood. This study analyzed 1586 stool samples from 1377 adults (CMD, CCD, and healthy controls) using 16S rRNA sequencing. Cohort 1 (n = 1168) was used for cross-sectional analysis, while cohort 2 (n = 209) underwent longitudinal assessment over approximately 13 months. The results demonstrated that probiotics promoted significant gut microbiota alterations across both cohorts. Probiotic supplementation significantly increased lactobacilli in the CMD, CCD, and H groups. In both cohorts, probiotic supplementation enhanced Butyricicoccus, Clostridium sensu stricto 1, and Coprococcus in H groups, enhanced Anaerostipes and Fusicatenibacter in CMD groups, and reduced Haemophilus and Lachnospira in CCD groups. Notably, long-term supplementation not only elevated Dorea, Eubacterium hallii group, and Blautia in all groups but also suppressed Klebsiella and Bilophila in the CMD and CCD groups. Enterotype analysis revealed that probiotics increased the proportion of enterotype 1 and transition probabilities from enterotype 2 to 1 in the CMD and CCD groups, demonstrating that CCD/CMD gut microbiota exhibited greater responsiveness to probiotic modulation. Overall, this study suggests probiotics’ role in modulating adult gut microbiota and their potential benefits in chronic metabolic and cardio-cerebrovascular diseases.
Keywords: probiotic supplementation; cross-sectional cohort; longitudinal cohort; gut microbiota; 16S rRNA gene sequencing probiotic supplementation; cross-sectional cohort; longitudinal cohort; gut microbiota; 16S rRNA gene sequencing

Share and Cite

MDPI and ACS Style

Wang, X.; Dong, W.; Liu, Q.; Zeng, X.; Liu, Y.; Li, Z.; Pan, Y.; Xiong, Q.; Lyu, N.; Zhu, B. Probiotic Supplementation Improves Gut Microbiota in Chronic Metabolic and Cardio-Cerebrovascular Diseases Among Chinese Adults over 60: Study Using Cross-Sectional and Longitudinal Cohorts. Microorganisms 2025, 13, 1507. https://doi.org/10.3390/microorganisms13071507

AMA Style

Wang X, Dong W, Liu Q, Zeng X, Liu Y, Li Z, Pan Y, Xiong Q, Lyu N, Zhu B. Probiotic Supplementation Improves Gut Microbiota in Chronic Metabolic and Cardio-Cerebrovascular Diseases Among Chinese Adults over 60: Study Using Cross-Sectional and Longitudinal Cohorts. Microorganisms. 2025; 13(7):1507. https://doi.org/10.3390/microorganisms13071507

Chicago/Turabian Style

Wang, Xi, Wanting Dong, Qiuying Liu, Xi Zeng, Yan Liu, Zheng Li, Yuanlong Pan, Qian Xiong, Na Lyu, and Baoli Zhu. 2025. "Probiotic Supplementation Improves Gut Microbiota in Chronic Metabolic and Cardio-Cerebrovascular Diseases Among Chinese Adults over 60: Study Using Cross-Sectional and Longitudinal Cohorts" Microorganisms 13, no. 7: 1507. https://doi.org/10.3390/microorganisms13071507

APA Style

Wang, X., Dong, W., Liu, Q., Zeng, X., Liu, Y., Li, Z., Pan, Y., Xiong, Q., Lyu, N., & Zhu, B. (2025). Probiotic Supplementation Improves Gut Microbiota in Chronic Metabolic and Cardio-Cerebrovascular Diseases Among Chinese Adults over 60: Study Using Cross-Sectional and Longitudinal Cohorts. Microorganisms, 13(7), 1507. https://doi.org/10.3390/microorganisms13071507

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop