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Microorganisms
Volume 11
Issue 5
10.3390/microorganisms11051110
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Article

Polyhydroxybutyrate Production from Methane and Carbon Dioxide by a Syntrophic Consortium of Methanotrophs with Oxygenic Photogranules without an External Oxygen Supply

by
Selim Ashoor
1,2,
Seong-Hoon Jun
2,
Han Do Ko
2,
Jinwon Lee
2,
Jérôme Hamelin
3,
Kim Milferstedt
3 and
Jeong-Geol Na
2,*
1
Department of Agricultural Microbiology, Faculty of Agriculture, Ain Shams University, Hadayek Shoubra, Cairo 11241, Egypt
2
Department of Chemical and Biomolecular Engineering, Sogang University, Seoul 04107, Republic of Korea
3
INRAE, University of Montpellier, LBE, 102 Avenue des Etangs, 11100 Narbonne, France
*
Author to whom correspondence should be addressed.
Microorganisms 2023, 11(5), 1110; https://doi.org/10.3390/microorganisms11051110
Submission received: 26 March 2023 / Revised: 16 April 2023 / Accepted: 22 April 2023 / Published: 24 April 2023
(This article belongs to the Special Issue Microbial Communities in Waste Treatment)
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Abstract

Here, a syntrophic process was developed to produce polyhydroxy-β-butyrate (PHB) from a gas stream containing CH4 and CO2 without an external oxygen supply using a combination of methanotrophs with the community of oxygenic photogranules (OPGs). The co-culture features of Methylomonas sp. DH-1 and Methylosinus trichosporium OB3b were evaluated under carbon-rich and carbon-lean conditions. The critical role of O2 in the syntrophy was confirmed through the sequencing of 16S rRNA gene fragments. Based on their carbon consumption rates and the adaptation to a poor environment, M. trichosporium OB3b with OPGs was selected for methane conversion and PHB production. Nitrogen limitation stimulated PHB accumulation in the methanotroph but hindered the growth of the syntrophic consortium. At 2.9 mM of the nitrogen source, 1.13 g/L of biomass and 83.0 mg/L of PHB could be obtained from simulated biogas. These results demonstrate that syntrophy has the potential to convert greenhouse gases into valuable products efficiently.
Keywords: greenhouse gas mitigation; polyhydroxybutyrate; syntrophic process; methanotrophy; oxygenic photogranules greenhouse gas mitigation; polyhydroxybutyrate; syntrophic process; methanotrophy; oxygenic photogranules

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MDPI and ACS Style

Ashoor, S.; Jun, S.-H.; Ko, H.D.; Lee, J.; Hamelin, J.; Milferstedt, K.; Na, J.-G. Polyhydroxybutyrate Production from Methane and Carbon Dioxide by a Syntrophic Consortium of Methanotrophs with Oxygenic Photogranules without an External Oxygen Supply. Microorganisms 2023, 11, 1110. https://doi.org/10.3390/microorganisms11051110

AMA Style

Ashoor S, Jun S-H, Ko HD, Lee J, Hamelin J, Milferstedt K, Na J-G. Polyhydroxybutyrate Production from Methane and Carbon Dioxide by a Syntrophic Consortium of Methanotrophs with Oxygenic Photogranules without an External Oxygen Supply. Microorganisms. 2023; 11(5):1110. https://doi.org/10.3390/microorganisms11051110

Chicago/Turabian Style

Ashoor, Selim, Seong-Hoon Jun, Han Do Ko, Jinwon Lee, Jérôme Hamelin, Kim Milferstedt, and Jeong-Geol Na. 2023. "Polyhydroxybutyrate Production from Methane and Carbon Dioxide by a Syntrophic Consortium of Methanotrophs with Oxygenic Photogranules without an External Oxygen Supply" Microorganisms 11, no. 5: 1110. https://doi.org/10.3390/microorganisms11051110

APA Style

Ashoor, S., Jun, S.-H., Ko, H. D., Lee, J., Hamelin, J., Milferstedt, K., & Na, J.-G. (2023). Polyhydroxybutyrate Production from Methane and Carbon Dioxide by a Syntrophic Consortium of Methanotrophs with Oxygenic Photogranules without an External Oxygen Supply. Microorganisms, 11(5), 1110. https://doi.org/10.3390/microorganisms11051110

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